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The map label for this gene is tyrS [H]

Identifier: 159900216

GI number: 159900216

Start: 4652965

End: 4654146

Strand: Direct

Name: tyrS [H]

Synonym: Haur_3699

Alternate gene names: 159900216

Gene position: 4652965-4654146 (Clockwise)

Preceding gene: 159900211

Following gene: 159900217

Centisome position: 73.31

GC content: 48.65

Gene sequence:

>1182_bases
ATGTTGACGATTGATGAAATTTTATCGCGGGGCGTGTCTGAGGTGATTGTTGAAGCCAATGTTCGCCGCAAATTGAATGC
TGGCAAACCATTACGCCTCAAATTGGGCATCGACCCAACCCGCAGCGATATTCATATTGGCCATGCAGTGGCGTTGCGCA
AGATGCGCCAATTTCAAGAGCTTGGCCATACCGTCGTTTTGATTGTGGGCGACTGGACAGCTCAAATCGGCGACCCCACG
GGCCGTGACGAAAGTCGCCCACGCTTGACGGTCGAAGAAACCAAAAGCAACGCCAAATATTACATGGATCAAGTGTTTAA
AGTGATTGATCCAGCCAAAACTGAAGTTCGCTGGCAAAGCGAATGGTTTGGCCAATTCGATCTTGAAAAAGCTTTTGGCT
TGATTGGACGCTTTACAGTTAACCAAATGTTGGCGCACGAAACCTTCCGCAAGCGCTACGAAGCTGGCCAGCAGTTAACC
GTGCTTGAGTTGATGTACCCAATGCTGCAAGCCTACGATTCGATTGCAATCAAGCCTGATATTGAGTTTGGTGGCACTGA
CCAAAAATTCAACATTTTGGCTGGGCGACAATTGATGGAACAAATGGGCATGGAGTCGCAGGATGTCATCCTCGTGCCAT
TAATCATTGGCACTGATGGCCGCAAGATGAGCAAAAGCTTCAACAACTCGGTTGATATTTTGATGTCACCTAACGATAAG
TATGGCAAAATTATGTCAATGGGCGATGATGTTTTGCCAGTTTATTACGAAGTTTGGAGCGATGCACCACTGGCTGAAGT
CAAGGCCATGTCAGAAGCAATCAAAGCTGGCAGCGTCAATCCCCGTGATCTCAAGATGCAACTGGCGCGTGATATTATTC
GCCAACTTGATGGTGAAGCGGCAGCGGCTGAGGCTGAAGCTGAATTTATTCGCGTGTTTCAACAACGCGATTTGCCAACC
GATATGCCCGAAGTAGCGCTCAGTGAAGCTGCCAACATTGTCGATTTGTTGGTTTCAACCAAGCTGGCCGCGAGCAAAGG
CGAGGCCCGCCGCTTGATCGATGGCGGTGGTGTGCGTTTGGCGGGCGAAAAAATCAGCAGCTACGATAGCCTCGTTCAAC
CAGTTGGCGAACAAATTTTACAAGTTGGGCGGCGCAAATTTGTGAAATTACTTGGTAATTAG

Upstream 100 bases:

>100_bases
GCTGCTAGAATAGTAGGTTTATTTGCTCCAACAGCGTTAAGGGGTTTGGCTCCGATGACTGCTTGGTGCTCGCCTAGCTC
AATTAGGAGTCTAAAAATTC

Downstream 100 bases:

>100_bases
CGCTAGATGCGAGGGTGCTGTGACGGGTTCGCGCTCGTCACAGCAAAAGGCTTATGGATCAACCGTTTCCGTTGGCGACC
GCCTGTTTTAAATGTTGGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLTIDEILSRGVSEVIVEANVRRKLNAGKPLRLKLGIDPTRSDIHIGHAVALRKMRQFQELGHTVVLIVGDWTAQIGDPT
GRDESRPRLTVEETKSNAKYYMDQVFKVIDPAKTEVRWQSEWFGQFDLEKAFGLIGRFTVNQMLAHETFRKRYEAGQQLT
VLELMYPMLQAYDSIAIKPDIEFGGTDQKFNILAGRQLMEQMGMESQDVILVPLIIGTDGRKMSKSFNNSVDILMSPNDK
YGKIMSMGDDVLPVYYEVWSDAPLAEVKAMSEAIKAGSVNPRDLKMQLARDIIRQLDGEAAAAEAEAEFIRVFQQRDLPT
DMPEVALSEAANIVDLLVSTKLAASKGEARRLIDGGGVRLAGEKISSYDSLVQPVGEQILQVGRRKFVKLLGN

Sequences:

>Translated_393_residues
MLTIDEILSRGVSEVIVEANVRRKLNAGKPLRLKLGIDPTRSDIHIGHAVALRKMRQFQELGHTVVLIVGDWTAQIGDPT
GRDESRPRLTVEETKSNAKYYMDQVFKVIDPAKTEVRWQSEWFGQFDLEKAFGLIGRFTVNQMLAHETFRKRYEAGQQLT
VLELMYPMLQAYDSIAIKPDIEFGGTDQKFNILAGRQLMEQMGMESQDVILVPLIIGTDGRKMSKSFNNSVDILMSPNDK
YGKIMSMGDDVLPVYYEVWSDAPLAEVKAMSEAIKAGSVNPRDLKMQLARDIIRQLDGEAAAAEAEAEFIRVFQQRDLPT
DMPEVALSEAANIVDLLVSTKLAASKGEARRLIDGGGVRLAGEKISSYDSLVQPVGEQILQVGRRKFVKLLGN
>Mature_393_residues
MLTIDEILSRGVSEVIVEANVRRKLNAGKPLRLKLGIDPTRSDIHIGHAVALRKMRQFQELGHTVVLIVGDWTAQIGDPT
GRDESRPRLTVEETKSNAKYYMDQVFKVIDPAKTEVRWQSEWFGQFDLEKAFGLIGRFTVNQMLAHETFRKRYEAGQQLT
VLELMYPMLQAYDSIAIKPDIEFGGTDQKFNILAGRQLMEQMGMESQDVILVPLIIGTDGRKMSKSFNNSVDILMSPNDK
YGKIMSMGDDVLPVYYEVWSDAPLAEVKAMSEAIKAGSVNPRDLKMQLARDIIRQLDGEAAAAEAEAEFIRVFQQRDLPT
DMPEVALSEAANIVDLLVSTKLAASKGEARRLIDGGGVRLAGEKISSYDSLVQPVGEQILQVGRRKFVKLLGN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=413, Percent_Identity=24.6973365617433, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787925, Length=397, Percent_Identity=27.7078085642317, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=207, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=431, Percent_Identity=27.8422273781903, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI20130383, Length=404, Percent_Identity=23.019801980198, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 43902; Mature: 43902

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIDEILSRGVSEVIVEANVRRKLNAGKPLRLKLGIDPTRSDIHIGHAVALRKMRQFQE
CCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
LGHTVVLIVGDWTAQIGDPTGRDESRPRLTVEETKSNAKYYMDQVFKVIDPAKTEVRWQS
CCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHHCCCHHHEEEHH
EWFGQFDLEKAFGLIGRFTVNQMLAHETFRKRYEAGQQLTVLELMYPMLQAYDSIAIKPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCC
IEFGGTDQKFNILAGRQLMEQMGMESQDVILVPLIIGTDGRKMSKSFNNSVDILMSPNDK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEECCCCC
YGKIMSMGDDVLPVYYEVWSDAPLAEVKAMSEAIKAGSVNPRDLKMQLARDIIRQLDGEA
CCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
AAAEAEAEFIRVFQQRDLPTDMPEVALSEAANIVDLLVSTKLAASKGEARRLIDGGGVRL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEE
AGEKISSYDSLVQPVGEQILQVGRRKFVKLLGN
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTIDEILSRGVSEVIVEANVRRKLNAGKPLRLKLGIDPTRSDIHIGHAVALRKMRQFQE
CCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
LGHTVVLIVGDWTAQIGDPTGRDESRPRLTVEETKSNAKYYMDQVFKVIDPAKTEVRWQS
CCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHHCCCHHHEEEHH
EWFGQFDLEKAFGLIGRFTVNQMLAHETFRKRYEAGQQLTVLELMYPMLQAYDSIAIKPD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCC
IEFGGTDQKFNILAGRQLMEQMGMESQDVILVPLIIGTDGRKMSKSFNNSVDILMSPNDK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHCCCCEEEEECCCCC
YGKIMSMGDDVLPVYYEVWSDAPLAEVKAMSEAIKAGSVNPRDLKMQLARDIIRQLDGEA
CCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
AAAEAEAEFIRVFQQRDLPTDMPEVALSEAANIVDLLVSTKLAASKGEARRLIDGGGVRL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEE
AGEKISSYDSLVQPVGEQILQVGRRKFVKLLGN
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA