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The map label for this gene is nucA [H]

Identifier: 159900161

GI number: 159900161

Start: 4582881

End: 4583720

Strand: Reverse

Name: nucA [H]

Synonym: Haur_3644

Alternate gene names: 159900161

Gene position: 4583720-4582881 (Counterclockwise)

Preceding gene: 159900162

Following gene: 159900158

Centisome position: 72.22

GC content: 49.4

Gene sequence:

>840_bases
ATGCGCCGTTTTTGGATTGGGATCATCACGCTGATTGTATTGTTGAGTGGCTGCGATGCTCAGCAATTTCTGGCCACGCC
AAAACCAGGTAGCTCCAATAATTCAAATCAACCGCAAGCCGAAGTGAATCACATGGCGTTGGGCAACCCTAGTGGAGCCT
TGCCCGAAACTAGCAGCAGCGATAACTACTTGATCCAGCGCCCAGAATATAGTTTGGCCTATAATCGCAAACTGAATATT
CTCAATTGGGTCAGTTGGCATCTCGATATTGGCGATTTAGGCAACACCGACCGCAGCCAATTTACCACCGACCCCGATTT
GCCCGAAGGCTGGTATCGAGTTAAGCCGACCGATTACACCAACAGCGGCTATGATCGCGGCCACATGTTGCCATCAGCCG
ATCGCACCGCCTCGGAAAGTATCAATCGCTCGGTGTTTTATATGACTAACATCGTGCCGCAAGCTCCCGACAATAACCAA
GGCCCATGGAAAGAATTTGAGGATTATTGCCGCGATTTGGTGCGTGATGGCAAGCAACTTTATCTGATCGCTGGCCCCGA
AGGCTCCGACGGCAGCATTGGCGACCCAAAAATTCGCGTGCCCAAGTATGTTTGGAAGATTGCGCTGGTGCTTGATGCTA
ACGATCAGATCAGCGATATCAACCGCAACACCGAAGTGATTGCGTTGCGAATGCCCAATCGCGATGGCATTCGCGATAAA
GATTGGCGCGATTATATCGTTTCGGTTGCCACCATCGAAGAAAAAACCAAATACACCTTTTTCACCAATTTGAGCAGCGA
ACTACAAAATAGCCTCAAGCAAAAAGTTGCTACTCCCTAA

Upstream 100 bases:

>100_bases
GTGTATTGTGAGAATGATTACCAATGCTCATGCACGTTTAAAGCGCCCAACTGTGCTACACTGCCTAGCAGAAACCATTC
TAGTTGCTGTGAGGATGTTT

Downstream 100 bases:

>100_bases
ATCATGCGTTGCACTCAACCCCTCGCCTACAATTGCCGAGGGGTTGGGGTGAGCAAGCGTCGCGCAATAGTTGCCCGAAT
TGTTTGGGCTTGGCCTGCCG

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRRFWIGIITLIVLLSGCDAQQFLATPKPGSSNNSNQPQAEVNHMALGNPSGALPETSSSDNYLIQRPEYSLAYNRKLNI
LNWVSWHLDIGDLGNTDRSQFTTDPDLPEGWYRVKPTDYTNSGYDRGHMLPSADRTASESINRSVFYMTNIVPQAPDNNQ
GPWKEFEDYCRDLVRDGKQLYLIAGPEGSDGSIGDPKIRVPKYVWKIALVLDANDQISDINRNTEVIALRMPNRDGIRDK
DWRDYIVSVATIEEKTKYTFFTNLSSELQNSLKQKVATP

Sequences:

>Translated_279_residues
MRRFWIGIITLIVLLSGCDAQQFLATPKPGSSNNSNQPQAEVNHMALGNPSGALPETSSSDNYLIQRPEYSLAYNRKLNI
LNWVSWHLDIGDLGNTDRSQFTTDPDLPEGWYRVKPTDYTNSGYDRGHMLPSADRTASESINRSVFYMTNIVPQAPDNNQ
GPWKEFEDYCRDLVRDGKQLYLIAGPEGSDGSIGDPKIRVPKYVWKIALVLDANDQISDINRNTEVIALRMPNRDGIRDK
DWRDYIVSVATIEEKTKYTFFTNLSSELQNSLKQKVATP
>Mature_279_residues
MRRFWIGIITLIVLLSGCDAQQFLATPKPGSSNNSNQPQAEVNHMALGNPSGALPETSSSDNYLIQRPEYSLAYNRKLNI
LNWVSWHLDIGDLGNTDRSQFTTDPDLPEGWYRVKPTDYTNSGYDRGHMLPSADRTASESINRSVFYMTNIVPQAPDNNQ
GPWKEFEDYCRDLVRDGKQLYLIAGPEGSDGSIGDPKIRVPKYVWKIALVLDANDQISDINRNTEVIALRMPNRDGIRDK
DWRDYIVSVATIEEKTKYTFFTNLSSELQNSLKQKVATP

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=221, Percent_Identity=28.9592760180996, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI53759134, Length=229, Percent_Identity=28.82096069869, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI224451075, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17505967, Length=160, Percent_Identity=30.625, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322253, Length=286, Percent_Identity=27.972027972028, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129899, Length=225, Percent_Identity=23.5555555555556, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581065, Length=213, Percent_Identity=24.4131455399061, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: NA

Molecular weight: Translated: 31581; Mature: 31581

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFWIGIITLIVLLSGCDAQQFLATPKPGSSNNSNQPQAEVNHMALGNPSGALPETSSS
CCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHCEEEECCCCCCCCCCCCC
DNYLIQRPEYSLAYNRKLNILNWVSWHLDIGDLGNTDRSQFTTDPDLPEGWYRVKPTDYT
CCEEEECCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
NSGYDRGHMLPSADRTASESINRSVFYMTNIVPQAPDNNQGPWKEFEDYCRDLVRDGKQL
CCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YLIAGPEGSDGSIGDPKIRVPKYVWKIALVLDANDQISDINRNTEVIALRMPNRDGIRDK
EEEECCCCCCCCCCCCCEECCCEEEEEEEEEECCCCHHHCCCCCEEEEEECCCCCCCCCC
DWRDYIVSVATIEEKTKYTFFTNLSSELQNSLKQKVATP
CHHHHHEEHEEECCCCEEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRFWIGIITLIVLLSGCDAQQFLATPKPGSSNNSNQPQAEVNHMALGNPSGALPETSSS
CCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHCEEEECCCCCCCCCCCCC
DNYLIQRPEYSLAYNRKLNILNWVSWHLDIGDLGNTDRSQFTTDPDLPEGWYRVKPTDYT
CCEEEECCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
NSGYDRGHMLPSADRTASESINRSVFYMTNIVPQAPDNNQGPWKEFEDYCRDLVRDGKQL
CCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YLIAGPEGSDGSIGDPKIRVPKYVWKIALVLDANDQISDINRNTEVIALRMPNRDGIRDK
EEEECCCCCCCCCCCCCEECCCEEEEEEEEEECCCCHHHCCCCCEEEEEECCCCCCCCCC
DWRDYIVSVATIEEKTKYTFFTNLSSELQNSLKQKVATP
CHHHHHEEHEEECCCCEEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]