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The map label for this gene is ycjG [C]

Identifier: 159900108

GI number: 159900108

Start: 4520659

End: 4521696

Strand: Reverse

Name: ycjG [C]

Synonym: Haur_3591

Alternate gene names: 159900108

Gene position: 4521696-4520659 (Counterclockwise)

Preceding gene: 159900109

Following gene: 159900107

Centisome position: 71.25

GC content: 53.37

Gene sequence:

>1038_bases
ATGGAATGGGCGATTTATCCGGTTGAACTACGCTTGCGCAACACGTTTCGGATTGCCCACGGAGCTAGCAATACCCGCCA
TAATGTGTTGTTGAATTTAGATGATGGCTGGGGCGAGGCCGCTGCTGTAGCCTACCACGGCGAAACTGCTGCTAAAATTC
AGGCTTGGCTGGAACGCTATCGCGAAACAATTACCAGCAGCTATGATCCAGCGGCAATTCATTGGCTCTTGGCCAAGCTC
GATTTTGAGAGCCGCGCCGCCCGCGCTGCTGTCGATTTAGCCTTGCATGATCGCTTAGGCCAACAACTTGGTGTGTCATT
GCGTCATCTTTTGGGCTTGAACGGCTTAGAACTCCCACAAACCTCAGTTACCTTGCCAATTGAAGAGCCTGAAGCTTTGC
GCCAACAAGCTTTGGCCGTAGCGCATTATCCAATTCTCAAGGTGAAGTTGGGCGGCCCTGCCGATTTAGCCAGCGTGGTT
TTGATTCGCGAAGCCGCACCCAACAGCCGCCTGCGGGTTGATGCCAATGCTGGTTGGAGCCGTGAAACCGCCGCCCAATT
GATTCCGGCGCTGGCTGAATTGGGCGTGGAGTTGATTGAGCAACCGTTGGCAGTTGATGATTTAGCGGGCTATGCTCAAC
TTAAAGCTGCCAACTATGGCGTGCCAATTTTTGCCGACGAGCCGATTAAAACTGCGGCTGATGTGGCGCGTTGGGCCAAG
GTGGTTGATGGCGTAAACCTCAAGTTGATGAAAACTGGCGGAATTGTCGGGGCGTGCGCAGCGATTGCCACCGCCAGAGC
CCATGATTTACAAGTGATGCTTGGGTGTATGATTGAAAGTAGCATCGGGGTTTCAGCGGCCTGTGCTTTGGCTGGCTTGG
CCGATTTCGTTGACCTTGATGGGCCATTATTGATCGCCAACGATCTGGCAACTGGATTGAACTTTGCAACCGCTACGATT
CAGCCAGCAGCAACGCCAGGTTTAGGCGTGCAAATTGACTGGACAGCACTAAACAGCGCCCGACTTGAAACACGCTAG

Upstream 100 bases:

>100_bases
TCTCGCTTGATCGCATTGATGAAGTGAATGCGCAGGTCTATCAAGCACAAGGGAACCCAGGGTTGGTGGTAATTCGGCGG
ATTCGCGAGGAGCAGTAGCC

Downstream 100 bases:

>100_bases
CATGCAGCCTACAACGACTAGAGAAGCGGTTTTAGGCCAGTCCTGCGGTCGGGCTGACAAGCGTACAGGGTATGCGTTGC
TAGACTTGTGTACCTACTGT

Product: mandelate racemase/muconate lactonizing protein

Products: 3-chloro-cis,cis-muconate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEWAIYPVELRLRNTFRIAHGASNTRHNVLLNLDDGWGEAAAVAYHGETAAKIQAWLERYRETITSSYDPAAIHWLLAKL
DFESRAARAAVDLALHDRLGQQLGVSLRHLLGLNGLELPQTSVTLPIEEPEALRQQALAVAHYPILKVKLGGPADLASVV
LIREAAPNSRLRVDANAGWSRETAAQLIPALAELGVELIEQPLAVDDLAGYAQLKAANYGVPIFADEPIKTAADVARWAK
VVDGVNLKLMKTGGIVGACAAIATARAHDLQVMLGCMIESSIGVSAACALAGLADFVDLDGPLLIANDLATGLNFATATI
QPAATPGLGVQIDWTALNSARLETR

Sequences:

>Translated_345_residues
MEWAIYPVELRLRNTFRIAHGASNTRHNVLLNLDDGWGEAAAVAYHGETAAKIQAWLERYRETITSSYDPAAIHWLLAKL
DFESRAARAAVDLALHDRLGQQLGVSLRHLLGLNGLELPQTSVTLPIEEPEALRQQALAVAHYPILKVKLGGPADLASVV
LIREAAPNSRLRVDANAGWSRETAAQLIPALAELGVELIEQPLAVDDLAGYAQLKAANYGVPIFADEPIKTAADVARWAK
VVDGVNLKLMKTGGIVGACAAIATARAHDLQVMLGCMIESSIGVSAACALAGLADFVDLDGPLLIANDLATGLNFATATI
QPAATPGLGVQIDWTALNSARLETR
>Mature_345_residues
MEWAIYPVELRLRNTFRIAHGASNTRHNVLLNLDDGWGEAAAVAYHGETAAKIQAWLERYRETITSSYDPAAIHWLLAKL
DFESRAARAAVDLALHDRLGQQLGVSLRHLLGLNGLELPQTSVTLPIEEPEALRQQALAVAHYPILKVKLGGPADLASVV
LIREAAPNSRLRVDANAGWSRETAAQLIPALAELGVELIEQPLAVDDLAGYAQLKAANYGVPIFADEPIKTAADVARWAK
VVDGVNLKLMKTGGIVGACAAIATARAHDLQVMLGCMIESSIGVSAACALAGLADFVDLDGPLLIANDLATGLNFATATI
QPAATPGLGVQIDWTALNSARLETR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=288, Percent_Identity=34.375, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 36730; Mature: 36730

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00099 THIOLASE_3 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWAIYPVELRLRNTFRIAHGASNTRHNVLLNLDDGWGEAAAVAYHGETAAKIQAWLERY
CCEEEEEEEEEEECEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHH
RETITSSYDPAAIHWLLAKLDFESRAARAAVDLALHDRLGQQLGVSLRHLLGLNGLELPQ
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TSVTLPIEEPEALRQQALAVAHYPILKVKLGGPADLASVVLIREAAPNSRLRVDANAGWS
CCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEECCCCCCEEEECCCCCC
RETAAQLIPALAELGVELIEQPLAVDDLAGYAQLKAANYGVPIFADEPIKTAADVARWAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEECCCCCEEEECCCCHHHHHHHHHHH
VVDGVNLKLMKTGGIVGACAAIATARAHDLQVMLGCMIESSIGVSAACALAGLADFVDLD
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GPLLIANDLATGLNFATATIQPAATPGLGVQIDWTALNSARLETR
CCEEEECHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MEWAIYPVELRLRNTFRIAHGASNTRHNVLLNLDDGWGEAAAVAYHGETAAKIQAWLERY
CCEEEEEEEEEEECEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHH
RETITSSYDPAAIHWLLAKLDFESRAARAAVDLALHDRLGQQLGVSLRHLLGLNGLELPQ
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TSVTLPIEEPEALRQQALAVAHYPILKVKLGGPADLASVVLIREAAPNSRLRVDANAGWS
CCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEECCCCCCEEEECCCCCC
RETAAQLIPALAELGVELIEQPLAVDDLAGYAQLKAANYGVPIFADEPIKTAADVARWAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHEEECCCCCEEEECCCCHHHHHHHHHHH
VVDGVNLKLMKTGGIVGACAAIATARAHDLQVMLGCMIESSIGVSAACALAGLADFVDLD
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GPLLIANDLATGLNFATATIQPAATPGLGVQIDWTALNSARLETR
CCEEEECHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]