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The map label for this gene is murF [H]

Identifier: 159900033

GI number: 159900033

Start: 4427041

End: 4428510

Strand: Reverse

Name: murF [H]

Synonym: Haur_3516

Alternate gene names: 159900033

Gene position: 4428510-4427041 (Counterclockwise)

Preceding gene: 159900034

Following gene: 159900032

Centisome position: 69.78

GC content: 54.56

Gene sequence:

>1470_bases
ATGCTAACCTTACAAACGGTCGTTGATGGGGTGAAAATTGCCAGCGAACCACGCCTGCAATTGCCCGCAGCCCTCGCCAA
TTTGCCAATTCGCAGTGCCTGCATCGATTCGCGTGAAGCCAGCGTTGATGGTTTGTTTGTGGCCTTACAGGGCGAACGGG
TCGATGGCCACGATTTTATTGCGGCAGCGGCGGCACGCGGCGTTCGGGCGGCTTTAGTCCAACGCGGGCGTTTGGCTGAT
CCCAGCCAGCTTGGTTTGGATCGACCGTTTGTGCTGCTCGACCCTGATCAAGGCCAGATTGCCAATGAAGCGCCAGCCAA
TGCCTTTTTTCTGATTAGTGTTGATCAGACCTTGACTGCGATGCATCGGTTGGCGACGCGCTATCGTCAACAGCAGGATT
TGGTAGTGGTGGGGATTACGGGTAGCGTTGGTAAAACCTCGACCAAAGAGGTGCTAGCGGCCATGCTTGCACCAACCATG
CGCACCCACAAAAGCCCACGCTCCTACAACAGCGAAAATACTTTGCCCTTGGTGGTGTTAGGAATTGAACCAGAACATCA
GATTGCAGTGCTCGAAATGGGCATCTACAACAAAGGCGATCTGGCTTTGCTAACAACGATTGCCCAGCCCCAAATTGGCA
TCGTGACCAACGTTGGCGCATCGCATTTGGAGCGTATGCACACGATCGAGAATGTGGCCGAGGCCAAAAGCGAATTGGTG
CAAGCCTTGCCCGCCGATGGTTGGGCGATTCTGAATTACGATGATCAGCGGGTGCGGGCGATGCAACAGGTGACCAAAGC
CCAAGTTTTTACCTATGGGCTTGACCCTGAAGCCGATTTGTGGGCCGATAACATCGAAAGCCGTGGCCTCGATGGCATTG
GCTTTGATGCCCATTATCGTGGCGAACAATGGCATATCAAATTGCCCTTGATTGGCTTGCACAGCGTGCACACGGCTTTG
GCGGCGGCAGCAGTCGGGATTGTGTTAGGGCTTTCATGGGATTCGCTGATTCAAGGGTTGCGCAGCAGCGCCCAATTGCG
CTTGCTCTCATATCCAGCAGTTGGCGGCGCAACCCTGATTGACGATACCTACAATGCCTCGCCTGTTTCCTGCATTGCGG
CGCTGAATTTATTGGCTGAACTACAAGGCCGCCGCATCGCTGTATTTGGCGATATGGCCGAGCTTGGCCCGCACGATGAA
TCGGGTCATCGTTTGGTCGGGGTGCGAGCCGCCGATGTGGTTGATCAATTATATGTAGTAGGCGAAAAAGCGCGTTGGAT
TGGCGAAGAAGCCATTGAAGTAGGCCTGCCTGCTGAACAAGTGCATGTGCTGCCTGATAAAGCTGCGCTGATTGCGGCGT
TGCAATCGACGCTCCAAGCGAATGATTACGTCCTTGTCAAAGGATCGCGTGCTGCTGCCATGGAAGCTGTTGCAAATGCC
TTGCAAGTTAGGCCGGAGGCTACGGCGTGA

Upstream 100 bases:

>100_bases
ATTATCAAATTCCGCCCAACCCTGATTTGGTGCATGAATGCCAAAAAGCCCAATAAGCAATGAATTAGCGCCATGCAACC
CATGGCTTTGAGATAGGCCT

Downstream 100 bases:

>100_bases
ATAACGAACAGGTCGTGATCGAGATTCAGCGAGCGCTGGTGCTCAAACTGGCTGAGGCGTTGTTGTTGGCCGGAGCGGCC
TTTATTTTGACTTTAGTGCT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLTLQTVVDGVKIASEPRLQLPAALANLPIRSACIDSREASVDGLFVALQGERVDGHDFIAAAAARGVRAALVQRGRLAD
PSQLGLDRPFVLLDPDQGQIANEAPANAFFLISVDQTLTAMHRLATRYRQQQDLVVVGITGSVGKTSTKEVLAAMLAPTM
RTHKSPRSYNSENTLPLVVLGIEPEHQIAVLEMGIYNKGDLALLTTIAQPQIGIVTNVGASHLERMHTIENVAEAKSELV
QALPADGWAILNYDDQRVRAMQQVTKAQVFTYGLDPEADLWADNIESRGLDGIGFDAHYRGEQWHIKLPLIGLHSVHTAL
AAAAVGIVLGLSWDSLIQGLRSSAQLRLLSYPAVGGATLIDDTYNASPVSCIAALNLLAELQGRRIAVFGDMAELGPHDE
SGHRLVGVRAADVVDQLYVVGEKARWIGEEAIEVGLPAEQVHVLPDKAALIAALQSTLQANDYVLVKGSRAAAMEAVANA
LQVRPEATA

Sequences:

>Translated_489_residues
MLTLQTVVDGVKIASEPRLQLPAALANLPIRSACIDSREASVDGLFVALQGERVDGHDFIAAAAARGVRAALVQRGRLAD
PSQLGLDRPFVLLDPDQGQIANEAPANAFFLISVDQTLTAMHRLATRYRQQQDLVVVGITGSVGKTSTKEVLAAMLAPTM
RTHKSPRSYNSENTLPLVVLGIEPEHQIAVLEMGIYNKGDLALLTTIAQPQIGIVTNVGASHLERMHTIENVAEAKSELV
QALPADGWAILNYDDQRVRAMQQVTKAQVFTYGLDPEADLWADNIESRGLDGIGFDAHYRGEQWHIKLPLIGLHSVHTAL
AAAAVGIVLGLSWDSLIQGLRSSAQLRLLSYPAVGGATLIDDTYNASPVSCIAALNLLAELQGRRIAVFGDMAELGPHDE
SGHRLVGVRAADVVDQLYVVGEKARWIGEEAIEVGLPAEQVHVLPDKAALIAALQSTLQANDYVLVKGSRAAAMEAVANA
LQVRPEATA
>Mature_489_residues
MLTLQTVVDGVKIASEPRLQLPAALANLPIRSACIDSREASVDGLFVALQGERVDGHDFIAAAAARGVRAALVQRGRLAD
PSQLGLDRPFVLLDPDQGQIANEAPANAFFLISVDQTLTAMHRLATRYRQQQDLVVVGITGSVGKTSTKEVLAAMLAPTM
RTHKSPRSYNSENTLPLVVLGIEPEHQIAVLEMGIYNKGDLALLTTIAQPQIGIVTNVGASHLERMHTIENVAEAKSELV
QALPADGWAILNYDDQRVRAMQQVTKAQVFTYGLDPEADLWADNIESRGLDGIGFDAHYRGEQWHIKLPLIGLHSVHTAL
AAAAVGIVLGLSWDSLIQGLRSSAQLRLLSYPAVGGATLIDDTYNASPVSCIAALNLLAELQGRRIAVFGDMAELGPHDE
SGHRLVGVRAADVVDQLYVVGEKARWIGEEAIEVGLPAEQVHVLPDKAALIAALQSTLQANDYVLVKGSRAAAMEAVANA
LQVRPEATA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=465, Percent_Identity=34.6236559139785, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 52325; Mature: 52325

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLQTVVDGVKIASEPRLQLPAALANLPIRSACIDSREASVDGLFVALQGERVDGHDFI
CEEHHHHHCCHHCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCCCCHHHH
AAAAARGVRAALVQRGRLADPSQLGLDRPFVLLDPDQGQIANEAPANAFFLISVDQTLTA
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCHHHHH
MHRLATRYRQQQDLVVVGITGSVGKTSTKEVLAAMLAPTMRTHKSPRSYNSENTLPLVVL
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
GIEPEHQIAVLEMGIYNKGDLALLTTIAQPQIGIVTNVGASHLERMHTIENVAEAKSELV
ECCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
QALPADGWAILNYDDQRVRAMQQVTKAQVFTYGLDPEADLWADNIESRGLDGIGFDAHYR
HHCCCCCEEEECCCHHHHHHHHHHHHHHEEEECCCCCCHHHHCCHHHCCCCCCCCCCEEC
GEQWHIKLPLIGLHSVHTALAAAAVGIVLGLSWDSLIQGLRSSAQLRLLSYPAVGGATLI
CCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCEEE
DDTYNASPVSCIAALNLLAELQGRRIAVFGDMAELGPHDESGHRLVGVRAADVVDQLYVV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCEEEEEHHHHHHHHHHHH
GEKARWIGEEAIEVGLPAEQVHVLPDKAALIAALQSTLQANDYVLVKGSRAAAMEAVANA
CCHHHHHCHHHHHCCCCHHHEEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
LQVRPEATA
HHCCCCCCC
>Mature Secondary Structure
MLTLQTVVDGVKIASEPRLQLPAALANLPIRSACIDSREASVDGLFVALQGERVDGHDFI
CEEHHHHHCCHHCCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCCCCHHHH
AAAAARGVRAALVQRGRLADPSQLGLDRPFVLLDPDQGQIANEAPANAFFLISVDQTLTA
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCHHHHH
MHRLATRYRQQQDLVVVGITGSVGKTSTKEVLAAMLAPTMRTHKSPRSYNSENTLPLVVL
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
GIEPEHQIAVLEMGIYNKGDLALLTTIAQPQIGIVTNVGASHLERMHTIENVAEAKSELV
ECCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
QALPADGWAILNYDDQRVRAMQQVTKAQVFTYGLDPEADLWADNIESRGLDGIGFDAHYR
HHCCCCCEEEECCCHHHHHHHHHHHHHHEEEECCCCCCHHHHCCHHHCCCCCCCCCCEEC
GEQWHIKLPLIGLHSVHTALAAAAVGIVLGLSWDSLIQGLRSSAQLRLLSYPAVGGATLI
CCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCEEE
DDTYNASPVSCIAALNLLAELQGRRIAVFGDMAELGPHDESGHRLVGVRAADVVDQLYVV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCEEEEEHHHHHHHHHHHH
GEKARWIGEEAIEVGLPAEQVHVLPDKAALIAALQSTLQANDYVLVKGSRAAAMEAVANA
CCHHHHHCHHHHHCCCCHHHEEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
LQVRPEATA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]