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The map label for this gene is murD [H]

Identifier: 159900031

GI number: 159900031

Start: 4424523

End: 4425821

Strand: Reverse

Name: murD [H]

Synonym: Haur_3514

Alternate gene names: 159900031

Gene position: 4425821-4424523 (Counterclockwise)

Preceding gene: 159900032

Following gene: 159900030

Centisome position: 69.74

GC content: 52.96

Gene sequence:

>1299_bases
ATGCTCGATTTACGCAATAAACGTATAACGGTGATGGGCTTAGGTGTTCATGGTGGCGGTTTAGGGGTCACGCGCTTTTT
GCTTGAGCAAGGAGCCCATGTGATTGTGACCGATCTCCGTAGCGCCGAGATTTTGCAGCCCTCGCTTGAGGCTTTGGCAG
GCTTACCAGTTGAATTTGTGCTGGGTGAGCATCGTGATCAAGATTTTGAGCGGGTCGATATGGTGATTCGCAACCCAGGT
GTGCCGCGTGAATCGCGTTATTTGCAATTGGCTCGCGCTGCTGGTGTGGCGATTGAAATGGAGATGACGCTGTTCTTTCG
GCTTTGCCCTGCGCCAATTATTGGCATCACCGGAACCAAGGGCAAAACCACCACCACTACCCTGACTGGGGCGATGTTGC
GCGAGGTTTATCCTGATACGGTGGTTGCGGGCAATTTGCGGGTTTCGGCTTTGGAACAATTGCCACGGATTACTGCCAAA
ACGCCAGTGGTGCTTGAGCTATCGTCGTGGCAATTGGAAGGCTTGGGCGAGGCCGGATTAAGCCCTGAGTATGCCTGTGT
CACCAATTTGTCACCTGACCATCTCGACCGTTATGGCTCGATGGCCGATTATGGATTAGCCAAGCAACAAATTTTTCTGT
ATCAAAGCCCTGATGATGTGGTAGTGCTGCCTAAGCATGATCTGATTGTCAATACGTGGGCCAATGACGCTCCAGGCCGA
GTGATTTGGTTTGATGGCAACGAAGGCTGGCCGATGGGTCAGTTGCAAGGCCAACATAATGCTTTGAATATTGCAGCGGC
GGCGGCTTTGGCTCAAGCCTATGGCGTGCCCGATGCCGCAATTCGCAAGGCCATTGAGCATTTTGCTGGGGTTGAGCATC
GTATGGAGTTGGTACGCGAGATCAACGGCGTGCGTTTGATCAACGATACGGCTGCTACCACTCCAACGGCTGCTGCTGCT
GCGCTCAATTCGATGACCAGCCCAGTAATTTTGATTGCTGGTGGGGCTGATAAAAAACTGGCGTGGACACCATTGGTTGA
AGCGATTCAACAATCGGCCCAATTGAAACGGCTGATTATCTTGGATGGCACGGGTTCGCCAGCCCTGAGTGCGGCGCTTG
GTACTGAGCAAGAGCGCTATCAATCGTTTGAGCAAGCGATTCGCGCCGCCTTTGCCGAGGCTGAAGCTGGCGATACCGTG
TTGCTCTCGCCTGGCGCTGCCAGTTTTGGCATGTTTCGCAATGAATTTCATCGCGGCGAAGAATTTCGCCGAATTGTTGG
CCTACTGACGGAAGCTTAA

Upstream 100 bases:

>100_bases
CGGCCTATCCAACGCCGATCGGCAATTAACGGGCGTTCGTAAGGTTGCTGGTAGAGTTAGCTTGATTCAATAGCAAAGTG
CTTAGCAAGTAAGGCAATGC

Downstream 100 bases:

>100_bases
AAATTATTTAGGAATATTGGGCTATAGCGCAATTGCTGGAAACCAGCCACGATAGTGAAGGTTCTGCGATTGAAGGTTAT
TGCCAAAAGCCTAAGTTCTT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLDLRNKRITVMGLGVHGGGLGVTRFLLEQGAHVIVTDLRSAEILQPSLEALAGLPVEFVLGEHRDQDFERVDMVIRNPG
VPRESRYLQLARAAGVAIEMEMTLFFRLCPAPIIGITGTKGKTTTTTLTGAMLREVYPDTVVAGNLRVSALEQLPRITAK
TPVVLELSSWQLEGLGEAGLSPEYACVTNLSPDHLDRYGSMADYGLAKQQIFLYQSPDDVVVLPKHDLIVNTWANDAPGR
VIWFDGNEGWPMGQLQGQHNALNIAAAAALAQAYGVPDAAIRKAIEHFAGVEHRMELVREINGVRLINDTAATTPTAAAA
ALNSMTSPVILIAGGADKKLAWTPLVEAIQQSAQLKRLIILDGTGSPALSAALGTEQERYQSFEQAIRAAFAEAEAGDTV
LLSPGAASFGMFRNEFHRGEEFRRIVGLLTEA

Sequences:

>Translated_432_residues
MLDLRNKRITVMGLGVHGGGLGVTRFLLEQGAHVIVTDLRSAEILQPSLEALAGLPVEFVLGEHRDQDFERVDMVIRNPG
VPRESRYLQLARAAGVAIEMEMTLFFRLCPAPIIGITGTKGKTTTTTLTGAMLREVYPDTVVAGNLRVSALEQLPRITAK
TPVVLELSSWQLEGLGEAGLSPEYACVTNLSPDHLDRYGSMADYGLAKQQIFLYQSPDDVVVLPKHDLIVNTWANDAPGR
VIWFDGNEGWPMGQLQGQHNALNIAAAAALAQAYGVPDAAIRKAIEHFAGVEHRMELVREINGVRLINDTAATTPTAAAA
ALNSMTSPVILIAGGADKKLAWTPLVEAIQQSAQLKRLIILDGTGSPALSAALGTEQERYQSFEQAIRAAFAEAEAGDTV
LLSPGAASFGMFRNEFHRGEEFRRIVGLLTEA
>Mature_432_residues
MLDLRNKRITVMGLGVHGGGLGVTRFLLEQGAHVIVTDLRSAEILQPSLEALAGLPVEFVLGEHRDQDFERVDMVIRNPG
VPRESRYLQLARAAGVAIEMEMTLFFRLCPAPIIGITGTKGKTTTTTLTGAMLREVYPDTVVAGNLRVSALEQLPRITAK
TPVVLELSSWQLEGLGEAGLSPEYACVTNLSPDHLDRYGSMADYGLAKQQIFLYQSPDDVVVLPKHDLIVNTWANDAPGR
VIWFDGNEGWPMGQLQGQHNALNIAAAAALAQAYGVPDAAIRKAIEHFAGVEHRMELVREINGVRLINDTAATTPTAAAA
ALNSMTSPVILIAGGADKKLAWTPLVEAIQQSAQLKRLIILDGTGSPALSAALGTEQERYQSFEQAIRAAFAEAEAGDTV
LLSPGAASFGMFRNEFHRGEEFRRIVGLLTEA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=455, Percent_Identity=32.967032967033, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46594; Mature: 46594

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLRNKRITVMGLGVHGGGLGVTRFLLEQGAHVIVTDLRSAEILQPSLEALAGLPVEFV
CCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEE
LGEHRDQDFERVDMVIRNPGVPRESRYLQLARAAGVAIEMEMTLFFRLCPAPIIGITGTK
ECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHCCCCEEEEECCC
GKTTTTTLTGAMLREVYPDTVVAGNLRVSALEQLPRITAKTPVVLELSSWQLEGLGEAGL
CCCEEHHHHHHHHHHHCCCCEEECCCCHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCC
SPEYACVTNLSPDHLDRYGSMADYGLAKQQIFLYQSPDDVVVLPKHDLIVNTWANDAPGR
CCCEEEEECCCHHHHHHHCCHHHCCCCEEEEEEEECCCCEEEECCCCEEEEECCCCCCCE
VIWFDGNEGWPMGQLQGQHNALNIAAAAALAQAYGVPDAAIRKAIEHFAGVEHRMELVRE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHH
INGVRLINDTAATTPTAAAAALNSMTSPVILIAGGADKKLAWTPLVEAIQQSAQLKRLII
CCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHCEEEEEE
LDGTGSPALSAALGTEQERYQSFEQAIRAAFAEAEAGDTVLLSPGAASFGMFRNEFHRGE
EECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCH
EFRRIVGLLTEA
HHHHHHHHHHCC
>Mature Secondary Structure
MLDLRNKRITVMGLGVHGGGLGVTRFLLEQGAHVIVTDLRSAEILQPSLEALAGLPVEFV
CCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEE
LGEHRDQDFERVDMVIRNPGVPRESRYLQLARAAGVAIEMEMTLFFRLCPAPIIGITGTK
ECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHCCCCEEEEECCC
GKTTTTTLTGAMLREVYPDTVVAGNLRVSALEQLPRITAKTPVVLELSSWQLEGLGEAGL
CCCEEHHHHHHHHHHHCCCCEEECCCCHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCC
SPEYACVTNLSPDHLDRYGSMADYGLAKQQIFLYQSPDDVVVLPKHDLIVNTWANDAPGR
CCCEEEEECCCHHHHHHHCCHHHCCCCEEEEEEEECCCCEEEECCCCEEEEECCCCCCCE
VIWFDGNEGWPMGQLQGQHNALNIAAAAALAQAYGVPDAAIRKAIEHFAGVEHRMELVRE
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHH
INGVRLINDTAATTPTAAAAALNSMTSPVILIAGGADKKLAWTPLVEAIQQSAQLKRLII
CCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHCEEEEEE
LDGTGSPALSAALGTEQERYQSFEQAIRAAFAEAEAGDTVLLSPGAASFGMFRNEFHRGE
EECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCH
EFRRIVGLLTEA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA