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The map label for this gene is zraS [H]

Identifier: 159899976

GI number: 159899976

Start: 4351458

End: 4353092

Strand: Reverse

Name: zraS [H]

Synonym: Haur_3459

Alternate gene names: 159899976

Gene position: 4353092-4351458 (Counterclockwise)

Preceding gene: 159899977

Following gene: 159899975

Centisome position: 68.59

GC content: 49.91

Gene sequence:

>1635_bases
ATGGCAAGTGGTCAAGAAACGATTCTAGTGATCGACGATAGCGAAGCAATTCGGGTCAAACTTCAGGCTCAATTGCGTTC
GCTGGGCTATCAGGTCGTGTTGGCTGAAACTGGGCGAAATGGGCTAAACGCGATTACCCAACATCATCCGCACCTCATTT
TGCTCGATTATCAGCTGCCTGATACCACTGGGTTGGATTTGCTGCGTAAGCTGCGCACCGATGGCAACACCGTGCCAATT
TTGCTGATGACTGCCGAAGGTTCCGAACGCATCGCCGTAACGGCCTTCAAAATGGGCGTGCGCGATTATTTGATCAAGCC
TTTTGAGCCGCAAGATGTTGCTCAGGCGATCGATCGGGCGCTGCGTGAGTGGCGTTTGCAACGTGAAAAAGAAATTTTGC
TTGGTCAATTACAAGGCCAAGTGCGCCAATTAACGGTTTTGCATCGGGTTGGTAAGGCGGTAACTGCCCAACTTGATGCC
AGCAATTTGCTTGAACGAATTGTTGAAGCCTCAGTTTTTCTTTCGAATGCCGATGAAGGTTTTGTGCAATTAATTGATGA
TAATCAGTTGGTTGTGCGAGCCTCACACAATATTAACCCATTGCATTTGCGTGAGCTAAGCAAACATACCGATTATGAAT
TGGCAACGCGCACAATTAAAACCAACAAACCAATTCGGATCAATTCCGAGCGCGATGGAATTCGGGTGCAAGCCAATTAT
TTGGCCCAAGCTGTGCTCATGGTGCCGTTGTTGGTGGGGACTGAAGCCTTGGGCGTGCTGACCGTGGCTGCGACAACCCA
TCGCCGCAATTTTGATGAAGGCGATGAGCGCTTGATGCAGATGCTGGCCGACTACGCCTCGATTGCCTTGCACAACGCCC
GCACCTACAGCGCACTGCGCGAAACCCAAGGTCGCTTGGTTGAAGCTGAAAAATTATCGGGCATGGGGCGCATGGCGGCT
TCGCTAGCCCACGAAATCAACAATCCACTGGCGATTATTCGCTCAGGCTTGGAGTTGGTGGCGCAACAACACACACCTGG
CACGGCGCTTGGCGATTTGGTGCAAGGACTGGATGAAGAAGTGGCGCGGATTGCGCGGTTGCTCTATACCTTGGTGAATT
TCTATCAGCCCAATAACGATGGTGTGCCGCCTGATCTCAATCATTTGATCATTTCACTGATGCACATCACCAAACCACAA
CTTGATAAAGCCAATGTTAAACTGTATCAGGAGTTGGCGACCGATCTGCCAGCGCCAAACATTAGCAGCGATGCTTGTAA
GCAAGTTTTGATCAATTTGGTGCGCAATGCGATTGATGCGATGCCCGATGGCGGCAAATTGACCATTCGCACGGCCCATC
AAAAGGGTCAAATTTTTGTCAATGTTGAAGATAGCGGAATTGGGATTCCGCCCGAACATCGCGAACGTATTTTTGAGCCA
TTCTTTAGCACTAAAGGTGTGACGGGAACGGGGCTTGGGCTTTCAGTTGTTTATGGTATTTTGCAACAAGTTGGCGGCGC
AATTTCGGTAGAGAGCATCGTGGATAAAGGCTCGAACTTTACCTTGCGCATTCCGGTTGCAGCCCAACGCAGCCAATCGC
CCGATCTTGATTCCGATGAATTGTTGATTGGTTAA

Upstream 100 bases:

>100_bases
TTCTGCCTTTTGACTTTTCATTCGTCCTTGCGTTCTACGCTTCTCTGTCGTATGATTTTGGCGAGAATCTCCTTGTTGAG
CATTGTAGAACGGCGAGCCT

Downstream 100 bases:

>100_bases
TCCGATGAATGAGTTGCCATTACAGATCTTGGTGGTCGATGACGAGCCACGCTTAAGTGGTCAGTTGCGGTTGTTATTGG
AAGGGCAAGGCTACAGCGTA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MASGQETILVIDDSEAIRVKLQAQLRSLGYQVVLAETGRNGLNAITQHHPHLILLDYQLPDTTGLDLLRKLRTDGNTVPI
LLMTAEGSERIAVTAFKMGVRDYLIKPFEPQDVAQAIDRALREWRLQREKEILLGQLQGQVRQLTVLHRVGKAVTAQLDA
SNLLERIVEASVFLSNADEGFVQLIDDNQLVVRASHNINPLHLRELSKHTDYELATRTIKTNKPIRINSERDGIRVQANY
LAQAVLMVPLLVGTEALGVLTVAATTHRRNFDEGDERLMQMLADYASIALHNARTYSALRETQGRLVEAEKLSGMGRMAA
SLAHEINNPLAIIRSGLELVAQQHTPGTALGDLVQGLDEEVARIARLLYTLVNFYQPNNDGVPPDLNHLIISLMHITKPQ
LDKANVKLYQELATDLPAPNISSDACKQVLINLVRNAIDAMPDGGKLTIRTAHQKGQIFVNVEDSGIGIPPEHRERIFEP
FFSTKGVTGTGLGLSVVYGILQQVGGAISVESIVDKGSNFTLRIPVAAQRSQSPDLDSDELLIG

Sequences:

>Translated_544_residues
MASGQETILVIDDSEAIRVKLQAQLRSLGYQVVLAETGRNGLNAITQHHPHLILLDYQLPDTTGLDLLRKLRTDGNTVPI
LLMTAEGSERIAVTAFKMGVRDYLIKPFEPQDVAQAIDRALREWRLQREKEILLGQLQGQVRQLTVLHRVGKAVTAQLDA
SNLLERIVEASVFLSNADEGFVQLIDDNQLVVRASHNINPLHLRELSKHTDYELATRTIKTNKPIRINSERDGIRVQANY
LAQAVLMVPLLVGTEALGVLTVAATTHRRNFDEGDERLMQMLADYASIALHNARTYSALRETQGRLVEAEKLSGMGRMAA
SLAHEINNPLAIIRSGLELVAQQHTPGTALGDLVQGLDEEVARIARLLYTLVNFYQPNNDGVPPDLNHLIISLMHITKPQ
LDKANVKLYQELATDLPAPNISSDACKQVLINLVRNAIDAMPDGGKLTIRTAHQKGQIFVNVEDSGIGIPPEHRERIFEP
FFSTKGVTGTGLGLSVVYGILQQVGGAISVESIVDKGSNFTLRIPVAAQRSQSPDLDSDELLIG
>Mature_543_residues
ASGQETILVIDDSEAIRVKLQAQLRSLGYQVVLAETGRNGLNAITQHHPHLILLDYQLPDTTGLDLLRKLRTDGNTVPIL
LMTAEGSERIAVTAFKMGVRDYLIKPFEPQDVAQAIDRALREWRLQREKEILLGQLQGQVRQLTVLHRVGKAVTAQLDAS
NLLERIVEASVFLSNADEGFVQLIDDNQLVVRASHNINPLHLRELSKHTDYELATRTIKTNKPIRINSERDGIRVQANYL
AQAVLMVPLLVGTEALGVLTVAATTHRRNFDEGDERLMQMLADYASIALHNARTYSALRETQGRLVEAEKLSGMGRMAAS
LAHEINNPLAIIRSGLELVAQQHTPGTALGDLVQGLDEEVARIARLLYTLVNFYQPNNDGVPPDLNHLIISLMHITKPQL
DKANVKLYQELATDLPAPNISSDACKQVLINLVRNAIDAMPDGGKLTIRTAHQKGQIFVNVEDSGIGIPPEHRERIFEPF
FSTKGVTGTGLGLSVVYGILQQVGGAISVESIVDKGSNFTLRIPVAAQRSQSPDLDSDELLIG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=34.4978165938865, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=32.6359832635983, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI145693157, Length=356, Percent_Identity=24.7191011235955, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=28.82096069869, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=29.4605809128631, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=122, Percent_Identity=31.1475409836066, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=36.8421052631579, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI48994928, Length=247, Percent_Identity=27.5303643724696, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=115, Percent_Identity=34.7826086956522, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=24.5689655172414, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=194, Percent_Identity=27.8350515463918, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787375, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=27.6150627615063, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=101, Percent_Identity=37.6237623762376, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786911, Length=101, Percent_Identity=37.6237623762376, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1788394, Length=122, Percent_Identity=34.4262295081967, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=25.3275109170306, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=29.5081967213115, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789808, Length=222, Percent_Identity=23.4234234234234, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=130, Percent_Identity=30, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=300, Percent_Identity=23, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=115, Percent_Identity=32.1739130434783, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787487, Length=104, Percent_Identity=31.7307692307692, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790861, Length=213, Percent_Identity=26.2910798122066, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788905, Length=126, Percent_Identity=31.7460317460317, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59845; Mature: 59713

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGQETILVIDDSEAIRVKLQAQLRSLGYQVVLAETGRNGLNAITQHHPHLILLDYQLP
CCCCCCEEEEEECCCEEEEEHHHHHHHCCEEEEEEECCCCHHHHHHCCCCEEEEEEECCC
DTTGLDLLRKLRTDGNTVPILLMTAEGSERIAVTAFKMGVRDYLIKPFEPQDVAQAIDRA
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHCHHHHHCCCCCHHHHHHHHHHH
LREWRLQREKEILLGQLQGQVRQLTVLHRVGKAVTAQLDASNLLERIVEASVFLSNADEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCC
FVQLIDDNQLVVRASHNINPLHLRELSKHTDYELATRTIKTNKPIRINSERDGIRVQANY
EEEEECCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHEEECCCCEEECCCCCCEEEEHHH
LAQAVLMVPLLVGTEALGVLTVAATTHRRNFDEGDERLMQMLADYASIALHNARTYSALR
HHHHHHHHHHHHCCHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ETQGRLVEAEKLSGMGRMAASLAHEINNPLAIIRSGLELVAQQHTPGTALGDLVQGLDEE
HHHCCEEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VARIARLLYTLVNFYQPNNDGVPPDLNHLIISLMHITKPQLDKANVKLYQELATDLPAPN
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
ISSDACKQVLINLVRNAIDAMPDGGKLTIRTAHQKGQIFVNVEDSGIGIPPEHRERIFEP
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
FFSTKGVTGTGLGLSVVYGILQQVGGAISVESIVDKGSNFTLRIPVAAQRSQSPDLDSDE
HHCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHCCCCCEEEEEEEHCCCCCCCCCCCCC
LLIG
EECC
>Mature Secondary Structure 
ASGQETILVIDDSEAIRVKLQAQLRSLGYQVVLAETGRNGLNAITQHHPHLILLDYQLP
CCCCCEEEEEECCCEEEEEHHHHHHHCCEEEEEEECCCCHHHHHHCCCCEEEEEEECCC
DTTGLDLLRKLRTDGNTVPILLMTAEGSERIAVTAFKMGVRDYLIKPFEPQDVAQAIDRA
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHCHHHHHCCCCCHHHHHHHHHHH
LREWRLQREKEILLGQLQGQVRQLTVLHRVGKAVTAQLDASNLLERIVEASVFLSNADEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCC
FVQLIDDNQLVVRASHNINPLHLRELSKHTDYELATRTIKTNKPIRINSERDGIRVQANY
EEEEECCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHEEECCCCEEECCCCCCEEEEHHH
LAQAVLMVPLLVGTEALGVLTVAATTHRRNFDEGDERLMQMLADYASIALHNARTYSALR
HHHHHHHHHHHHCCHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ETQGRLVEAEKLSGMGRMAASLAHEINNPLAIIRSGLELVAQQHTPGTALGDLVQGLDEE
HHHCCEEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VARIARLLYTLVNFYQPNNDGVPPDLNHLIISLMHITKPQLDKANVKLYQELATDLPAPN
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
ISSDACKQVLINLVRNAIDAMPDGGKLTIRTAHQKGQIFVNVEDSGIGIPPEHRERIFEP
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
FFSTKGVTGTGLGLSVVYGILQQVGGAISVESIVDKGSNFTLRIPVAAQRSQSPDLDSDE
HHCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHCCCCCEEEEEEEHCCCCCCCCCCCCC
LLIG
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]