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The map label for this gene is braC [H]

Identifier: 159899525

GI number: 159899525

Start: 3795932

End: 3797155

Strand: Reverse

Name: braC [H]

Synonym: Haur_3006

Alternate gene names: 159899525

Gene position: 3797155-3795932 (Counterclockwise)

Preceding gene: 159899527

Following gene: 159899524

Centisome position: 59.83

GC content: 50.25

Gene sequence:

>1224_bases
ATGAAACGTTTCCTTTCATTCTCGCTCGTCTTGACCATGATCATGGTGATTTTGGCCGCATGTGGCACCCAAACTGGTGG
AACAACCAAAACTACCGTCACGATTGTTAGTAGCTTACCCCGCACCGGTTCCTCAAAAGGACAAACAGATACCATTGTAA
ACGCCATTAAGATGCGTTTAGAAGAAGCCAAATACACCGCGTGCGATGGGAATGTTGAGATCAAGTATGAAGATCTTGAC
GATGCAACCGCAGCTAAAGGCGCTTGGGATGAAGCGAAAGAAGCCGAAAACGCTAATGCTGCTGCTTCAAACAAAGATGT
GATGGTCTATATCGGTACCTTCAACTCAGGTGCTGCCAAGATCTCGATTCCGATCTTGAACAATGCTGGGATTGTGATGA
TCAGCCCTGCCAACACCTACCCTGGCTTGACCAAGGATGGCAAAGGTGCTGAAGGCGAACCAGCAGTTTACTATCCCAAA
GGTACGCGCAACTACACCCGCGTGGTGCCAGCCGACGACTTGCAAGGCGCTGCCGCAGGCAACTGGGCCAAAGAATTGGG
CGTAACCAATGTCTACATCTTGGATGATACCGAGTTGTATGGCAAAGGGATTGCTGACGTATTTGCTGCTACCGCTGAAA
AGAATGGCATCAAGATTGCAGGTCGCGATAGCATCGATGCCAAAGCTAGCGATTACAAGGCGTTGATGTCGAAAATCGCC
GCTACCAACCCCGATATGATCTACTTCGGTGGCATCACCCAAAGCAACGCAGGCCAATTGGTCAAAGACATGCGGGCTGC
TGGGATGACCGCTGATAAAGTTAAGTTCATGGGTCCAGACGGGATCTTCGAACAAGCCTTTATCGACGCTGCTGGTGCTG
ATAACGCTGAAGGCGTTTATGCAACCTTCGGTGGCGTTCCACCAGCCAAGCTTGAAGGCAAAGGTGCCGAATGGTACAAC
GCCTACAAGGCCAAATTCAACGCTGAACCAGAAGCCTACGCTGTCTACGGCTACGAATCAACCAACGTGGCGTTGGCTGC
GATCAACCAATCATGTGCTAGCATCAACCGCGAAACCATCTTGAAGAACGTTTTCGCAACCAAAGATTTTGATGGCGTGT
TGGGCAAATGGGGCTTCGATGCAAGTGGCGACACGACATTGACCCAAATGAGCGGCCAACAAATCAAGGGTGGCGCGTTC
GAATTCGTTCAAGTCCTCAAGTAA

Upstream 100 bases:

>100_bases
GCTTTTAAGGAGGTTTTTGCCTCATCACTAAAACCATGTATGGCTTTGGGCTAGCCAGTTTTAGGCTTTCCTCGCTTTTA
AACAAGGAGCAACTGGAATT

Downstream 100 bases:

>100_bases
GATTTGATACCGTTTCCAAGGGACGATCCTCCGCTGATCGTCCCTTTCCCCTGTGTGGATTGTGTCGTTGCCAGCCCCTT
GCTGGGTACAGAGGAATAAC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKRFLSFSLVLTMIMVILAACGTQTGGTTKTTVTIVSSLPRTGSSKGQTDTIVNAIKMRLEEAKYTACDGNVEIKYEDLD
DATAAKGAWDEAKEAENANAAASNKDVMVYIGTFNSGAAKISIPILNNAGIVMISPANTYPGLTKDGKGAEGEPAVYYPK
GTRNYTRVVPADDLQGAAAGNWAKELGVTNVYILDDTELYGKGIADVFAATAEKNGIKIAGRDSIDAKASDYKALMSKIA
ATNPDMIYFGGITQSNAGQLVKDMRAAGMTADKVKFMGPDGIFEQAFIDAAGADNAEGVYATFGGVPPAKLEGKGAEWYN
AYKAKFNAEPEAYAVYGYESTNVALAAINQSCASINRETILKNVFATKDFDGVLGKWGFDASGDTTLTQMSGQQIKGGAF
EFVQVLK

Sequences:

>Translated_407_residues
MKRFLSFSLVLTMIMVILAACGTQTGGTTKTTVTIVSSLPRTGSSKGQTDTIVNAIKMRLEEAKYTACDGNVEIKYEDLD
DATAAKGAWDEAKEAENANAAASNKDVMVYIGTFNSGAAKISIPILNNAGIVMISPANTYPGLTKDGKGAEGEPAVYYPK
GTRNYTRVVPADDLQGAAAGNWAKELGVTNVYILDDTELYGKGIADVFAATAEKNGIKIAGRDSIDAKASDYKALMSKIA
ATNPDMIYFGGITQSNAGQLVKDMRAAGMTADKVKFMGPDGIFEQAFIDAAGADNAEGVYATFGGVPPAKLEGKGAEWYN
AYKAKFNAEPEAYAVYGYESTNVALAAINQSCASINRETILKNVFATKDFDGVLGKWGFDASGDTTLTQMSGQQIKGGAF
EFVQVLK
>Mature_407_residues
MKRFLSFSLVLTMIMVILAACGTQTGGTTKTTVTIVSSLPRTGSSKGQTDTIVNAIKMRLEEAKYTACDGNVEIKYEDLD
DATAAKGAWDEAKEAENANAAASNKDVMVYIGTFNSGAAKISIPILNNAGIVMISPANTYPGLTKDGKGAEGEPAVYYPK
GTRNYTRVVPADDLQGAAAGNWAKELGVTNVYILDDTELYGKGIADVFAATAEKNGIKIAGRDSIDAKASDYKALMSKIA
ATNPDMIYFGGITQSNAGQLVKDMRAAGMTADKVKFMGPDGIFEQAFIDAAGADNAEGVYATFGGVPPAKLEGKGAEWYN
AYKAKFNAEPEAYAVYGYESTNVALAAINQSCASINRETILKNVFATKDFDGVLGKWGFDASGDTTLTQMSGQQIKGGAF
EFVQVLK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=304, Percent_Identity=27.3026315789474, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789867, Length=219, Percent_Identity=27.8538812785388, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43015; Mature: 43015

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFLSFSLVLTMIMVILAACGTQTGGTTKTTVTIVSSLPRTGSSKGQTDTIVNAIKMRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EEAKYTACDGNVEIKYEDLDDATAAKGAWDEAKEAENANAAASNKDVMVYIGTFNSGAAK
HHCCEEEECCCEEEEEECCCCCHHCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCEE
ISIPILNNAGIVMISPANTYPGLTKDGKGAEGEPAVYYPKGTRNYTRVVPADDLQGAAAG
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCC
NWAKELGVTNVYILDDTELYGKGIADVFAATAEKNGIKIAGRDSIDAKASDYKALMSKIA
CHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
ATNPDMIYFGGITQSNAGQLVKDMRAAGMTADKVKFMGPDGIFEQAFIDAAGADNAEGVY
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHCCCCCCCCEEE
ATFGGVPPAKLEGKGAEWYNAYKAKFNAEPEAYAVYGYESTNVALAAINQSCASINRETI
EEECCCCCCCCCCCCCHHHHHHHHEECCCCCEEEEEEECCCCEEEEEECHHHHHCCHHHH
LKNVFATKDFDGVLGKWGFDASGDTTLTQMSGQQIKGGAFEFVQVLK
HHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKRFLSFSLVLTMIMVILAACGTQTGGTTKTTVTIVSSLPRTGSSKGQTDTIVNAIKMRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EEAKYTACDGNVEIKYEDLDDATAAKGAWDEAKEAENANAAASNKDVMVYIGTFNSGAAK
HHCCEEEECCCEEEEEECCCCCHHCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCEE
ISIPILNNAGIVMISPANTYPGLTKDGKGAEGEPAVYYPKGTRNYTRVVPADDLQGAAAG
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCC
NWAKELGVTNVYILDDTELYGKGIADVFAATAEKNGIKIAGRDSIDAKASDYKALMSKIA
CHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
ATNPDMIYFGGITQSNAGQLVKDMRAAGMTADKVKFMGPDGIFEQAFIDAAGADNAEGVY
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHCCCCCCCCEEE
ATFGGVPPAKLEGKGAEWYNAYKAKFNAEPEAYAVYGYESTNVALAAINQSCASINRETI
EEECCCCCCCCCCCCCHHHHHHHHEECCCCCEEEEEEECCCCEEEEEECHHHHHCCHHHH
LKNVFATKDFDGVLGKWGFDASGDTTLTQMSGQQIKGGAFEFVQVLK
HHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]