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The map label for this gene is dapE [H]

Identifier: 159899352

GI number: 159899352

Start: 3601368

End: 3602699

Strand: Reverse

Name: dapE [H]

Synonym: Haur_2833

Alternate gene names: 159899352

Gene position: 3602699-3601368 (Counterclockwise)

Preceding gene: 159899363

Following gene: 159899350

Centisome position: 56.77

GC content: 51.95

Gene sequence:

>1332_bases
GTGGTTGCTCCCCTATCCACTCTCGATGAACTGATTTTGCACTATGTCCGTGCCTTGGTAGCCTTACCAAGTGTTACTGG
CGATGACCCAGCCTTGGAACAGGCTGCGCCGACGATTGCTGATATATTGCGCGGCCTTGGGTTTCAGGTCAATGTGCACC
CAACTGAAGGTGCACCGATTATTTTGGCCCATCGGCCTGGCAAAAGCGCTCAACGCTTGCTCTTTTTTAATCACTACGAT
GTGATGCCAGCTGGGGTCTGGCGTGATTGGTTTCATGAGCCGTTTACCTTGGCTGAGCGCGAAGGGCTGCTCTATGGGCG
TGGCGTTGCCAACGATAAAGGCAATTTAGCTGCGCGAATTGCTGCTGTGGCTCAAATTTTGGCCGAAACTGGCGATCTCC
CAGTTGGTGTGACGTTTTTAATTGAAGGTGACGGGCTGAGTGGTAGCCCATCATTAGCTAATTTGGTTGCCGATCAAGCT
AATCATTTAACTGCCGATTTGGTAGTTGGCTATGGTGGCATGCTCGATCAAGCGCGTTTGCCCTACTTGTATGCTGGGGT
TCGTGGGCGCTTGTTGGTACGCTTGCGAGCTGAGGGTGCTAAAATTCCGATCGGTGCTGATATGGCGACCAGTGTACCCA
ACCCGGCTTGGCGCATCCTTTGGGCGGTCAATCGGATTAAAAATGATAGCGAAGAAGTGACGATTGATGGGTTTTATGAT
GCGGTTGTGCCACCAAGCCGCGAGGCCAATAAGCTCACTCGTGGCTTGCAGCTCGATGAGGAAACGCGACTAAAAGCTTG
GGGCATGCCGCAATTTTTATTTGGCATGACCGGGGCTGGTCTGGCTCGGGCTGAAACCTTCAACCCAACCTGTAATGTTG
CCGGATTAACGGTTGATACCGGTCATAGCCCCCCTCCAACCATCCCTGCCAGTGCCGAAGTGCTGCTCGATTTCAGTCTT
GTGCCTGAGCAACGCCCAACCGAAGTTGCCCGTTTGTTGCGTGAGCACCTCAATAGTGCCGATTTCCATGATGTGCATTT
GGAGATTATCAAGGGTGCCTATCCGCCCGCAATGAATGCGTTGAGCACACCATTACTCAATAGTTTAGCGACGGCAATTG
AACAGGTTTATGGCTCAACTCCGCAAATTGTGCCGCTTGCGCCATTCTCGGTGCCGCTACACCTTTTTACCGCTGGGATG
AATGTGCCAGCCGTTGCCTTGGGAATTCAACGCCCCGATAGCAACGTTCGGGTCATCAATGAACATATTCAGTTGGCCGA
TCTCAAAGCGATGGCTAGTTTGATTCAACAACTGATTATCGGCCTTGGCTAA

Upstream 100 bases:

>100_bases
CCAAAAGTTTGCTAGCGATTGTCGTTTTGGCTTGGCGGTTATCCGTTATAATGGAGATACCCGCTTGATATTGTTCGGCG
TGCGATTGCGAGGAAGCTCC

Downstream 100 bases:

>100_bases
ATCGATCAAGTTACGCTGCGATAGGTTATAAAAGGGTTGGTTCTTTACAATCATTGGCGATCACCACCCTTCCGTCCCGC
CTCAAGGCGGGAGACGCAGG

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVAPLSTLDELILHYVRALVALPSVTGDDPALEQAAPTIADILRGLGFQVNVHPTEGAPIILAHRPGKSAQRLLFFNHYD
VMPAGVWRDWFHEPFTLAEREGLLYGRGVANDKGNLAARIAAVAQILAETGDLPVGVTFLIEGDGLSGSPSLANLVADQA
NHLTADLVVGYGGMLDQARLPYLYAGVRGRLLVRLRAEGAKIPIGADMATSVPNPAWRILWAVNRIKNDSEEVTIDGFYD
AVVPPSREANKLTRGLQLDEETRLKAWGMPQFLFGMTGAGLARAETFNPTCNVAGLTVDTGHSPPPTIPASAEVLLDFSL
VPEQRPTEVARLLREHLNSADFHDVHLEIIKGAYPPAMNALSTPLLNSLATAIEQVYGSTPQIVPLAPFSVPLHLFTAGM
NVPAVALGIQRPDSNVRVINEHIQLADLKAMASLIQQLIIGLG

Sequences:

>Translated_443_residues
MVAPLSTLDELILHYVRALVALPSVTGDDPALEQAAPTIADILRGLGFQVNVHPTEGAPIILAHRPGKSAQRLLFFNHYD
VMPAGVWRDWFHEPFTLAEREGLLYGRGVANDKGNLAARIAAVAQILAETGDLPVGVTFLIEGDGLSGSPSLANLVADQA
NHLTADLVVGYGGMLDQARLPYLYAGVRGRLLVRLRAEGAKIPIGADMATSVPNPAWRILWAVNRIKNDSEEVTIDGFYD
AVVPPSREANKLTRGLQLDEETRLKAWGMPQFLFGMTGAGLARAETFNPTCNVAGLTVDTGHSPPPTIPASAEVLLDFSL
VPEQRPTEVARLLREHLNSADFHDVHLEIIKGAYPPAMNALSTPLLNSLATAIEQVYGSTPQIVPLAPFSVPLHLFTAGM
NVPAVALGIQRPDSNVRVINEHIQLADLKAMASLIQQLIIGLG
>Mature_443_residues
MVAPLSTLDELILHYVRALVALPSVTGDDPALEQAAPTIADILRGLGFQVNVHPTEGAPIILAHRPGKSAQRLLFFNHYD
VMPAGVWRDWFHEPFTLAEREGLLYGRGVANDKGNLAARIAAVAQILAETGDLPVGVTFLIEGDGLSGSPSLANLVADQA
NHLTADLVVGYGGMLDQARLPYLYAGVRGRLLVRLRAEGAKIPIGADMATSVPNPAWRILWAVNRIKNDSEEVTIDGFYD
AVVPPSREANKLTRGLQLDEETRLKAWGMPQFLFGMTGAGLARAETFNPTCNVAGLTVDTGHSPPPTIPASAEVLLDFSL
VPEQRPTEVARLLREHLNSADFHDVHLEIIKGAYPPAMNALSTPLLNSLATAIEQVYGSTPQIVPLAPFSVPLHLFTAGM
NVPAVALGIQRPDSNVRVINEHIQLADLKAMASLIQQLIIGLG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=343, Percent_Identity=26.8221574344023, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI271398239, Length=339, Percent_Identity=25.3687315634218, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1790395, Length=116, Percent_Identity=36.2068965517241, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17563146, Length=360, Percent_Identity=26.1111111111111, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193211059, Length=389, Percent_Identity=24.4215938303342, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319758, Length=467, Percent_Identity=23.5546038543897, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321159, Length=401, Percent_Identity=25.4364089775561, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585880, Length=293, Percent_Identity=25.2559726962457, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47568; Mature: 47568

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPLSTLDELILHYVRALVALPSVTGDDPALEQAAPTIADILRGLGFQVNVHPTEGAPI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHCCCCEEEEECCCCCEE
ILAHRPGKSAQRLLFFNHYDVMPAGVWRDWFHEPFTLAEREGLLYGRGVANDKGNLAARI
EEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHCCCCEEECCCCCCCCCHHHHH
AAVAQILAETGDLPVGVTFLIEGDGLSGSPSLANLVADQANHLTADLVVGYGGMLDQARL
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHCCCCCCCCCCCC
PYLYAGVRGRLLVRLRAEGAKIPIGADMATSVPNPAWRILWAVNRIKNDSEEVTIDGFYD
CEEEECCCCEEEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECEEC
AVVPPSREANKLTRGLQLDEETRLKAWGMPQFLFGMTGAGLARAETFNPTCNVAGLTVDT
CCCCCCCHHHHHHHCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEC
GHSPPPTIPASAEVLLDFSLVPEQRPTEVARLLREHLNSADFHDVHLEIIKGAYPPAMNA
CCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
LSTPLLNSLATAIEQVYGSTPQIVPLAPFSVPLHLFTAGMNVPAVALGIQRPDSNVRVIN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHEECCCCCCEEEEEEECCCCCCEEEH
EHIQLADLKAMASLIQQLIIGLG
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAPLSTLDELILHYVRALVALPSVTGDDPALEQAAPTIADILRGLGFQVNVHPTEGAPI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHCCCCEEEEECCCCCEE
ILAHRPGKSAQRLLFFNHYDVMPAGVWRDWFHEPFTLAEREGLLYGRGVANDKGNLAARI
EEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHCCCCEEECCCCCCCCCHHHHH
AAVAQILAETGDLPVGVTFLIEGDGLSGSPSLANLVADQANHLTADLVVGYGGMLDQARL
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHCCCCCCCCCCCC
PYLYAGVRGRLLVRLRAEGAKIPIGADMATSVPNPAWRILWAVNRIKNDSEEVTIDGFYD
CEEEECCCCEEEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECEEC
AVVPPSREANKLTRGLQLDEETRLKAWGMPQFLFGMTGAGLARAETFNPTCNVAGLTVDT
CCCCCCCHHHHHHHCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEC
GHSPPPTIPASAEVLLDFSLVPEQRPTEVARLLREHLNSADFHDVHLEIIKGAYPPAMNA
CCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
LSTPLLNSLATAIEQVYGSTPQIVPLAPFSVPLHLFTAGMNVPAVALGIQRPDSNVRVIN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHEECCCCCCEEEEEEECCCCCCEEEH
EHIQLADLKAMASLIQQLIIGLG
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA