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The map label for this gene is otsA [H]

Identifier: 159899348

GI number: 159899348

Start: 3596865

End: 3598325

Strand: Reverse

Name: otsA [H]

Synonym: Haur_2829

Alternate gene names: 159899348

Gene position: 3598325-3596865 (Counterclockwise)

Preceding gene: 159899349

Following gene: 159899347

Centisome position: 56.7

GC content: 42.37

Gene sequence:

>1461_bases
ATGCAAGAGTTACCAAATCGACTGATGATCGTCTCGAATCGGCTGCCGATTTCGATTGCCGAACAAGCCGATGGCACGCC
TACCATCAAGCCAGCTTCGGGTGGCTTGGTCACTGCCCTCGACCCGATTTTGCGCCAAGCACGTGGAACCTGGATCGGCT
GGTCGGGCACGAATGAGGCGGATCAAACGGTCGATCAATTACTCGAACATGCTGCCAATTCGATTGGTTATAGCATGCAT
GCGATTCACCTGAGCCAAGAGGAACAGGAAAAATTTTATTTGGGCTTTTCGAATGAGATTATCTGGCCATTATTTCATGA
TTTGCAGTCGCGTTGTAATTTCGACCCAAGCTATTGGACAACCTACGAGCAAGTTAATCGTAAATATGCTGAAACAATCG
CCCAACATTATCGTGATGATACTTATATTTGGGTGCAGGATTATCAATTAATTAATGTTGCTTGTGAGTTGCGCCAGCTG
GGAATTGATGCGCATATTGGATTCTTCTTGCACATTCCCTTTCCGCATATCGATATTTTCCTGAAATTACCGTGGCGCGA
GCAAATTCTGAATGGCTTGTTGGAATACGATTTACTAGGTTTTCAGACTAATCGTGATTTGAATAATTTTTTGATTTGTG
TTGAAGCATTATTACCTGATGTTGAGATCGATCGCTCTGGTGAGTATCCCTTAATTTTGCATGCAGATCGAAATCCTACC
AAAGTTGGCTTCTTTCCAATTAGCATTGATCCCCAAGAATTTCGCGCCGCAGCGCATACGCCAAATGTTGATAATTTATA
TAGCCAGATTCGTGAGGCTTTTTCAGAACGTAAAATCATTCTTGGGGTTGACCGTTTAGATTATACCAAAGGGATTCCTG
ATCGCTTACAAGCATTTAGTGCATTGCTCGAAACCTATCCTGAGCTACAACAAAAAATTTCATTAACTCAAGTTGTTGTA
CCAAGCCGCGAAGATATTAGTGAATACGCCGATTTGAAGCAAACAATTCAGCAATTAGTCAGTGAAATTAATGGTAAATA
TACCAAAATCGGTTGGGTGCCAATTCATTATATTTTTCGCTCATTACCACGCGAAGAATTATTAGCTTATTATCGCGCTG
CTCATGCCGCCTTAATCACACCATTAAAGGATGGCATGAATCTTGTAGCAAAGGAATATGCGGTCTGTGGGCCTGATGAT
GGCGTACTGATCTTAAGTGAATTTGCTGGGGCGGCAATGCAATTATCCGAACACTCATTGACGGTTAATCCCTATGATAT
TAATGGCACAGCTCAAGCACTTTATAATGCGGTCACAATGCCCCGCTTAGAACGGATTGAACATATTAGCAAGCTACGGC
ATTCGATCAATCAATATGATATTTATTGGTGGGTCGATACATTTTTGCAAGCCGCCAATGTCAAGCGTTCGGGGGCAATT
GAACAAGGATCAAGTTTATAA

Upstream 100 bases:

>100_bases
TGCTCAACGTCGTGCTGGATTTGCCTGCAATGCTAATGTATTGCTGTGTATTGTGCTGTGTATTTATATCAAACTGTGTT
TTTAAGGAGGATGGCAAATC

Downstream 100 bases:

>100_bases
GTATATATGCTACTAAAGGAGTTTTATGCAAAACGATTGGTATAAAGATGCAATCTTTTACGAATTGCATGTACGAGCTT
TTCAGGATAGCAATGGCGAT

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MQELPNRLMIVSNRLPISIAEQADGTPTIKPASGGLVTALDPILRQARGTWIGWSGTNEADQTVDQLLEHAANSIGYSMH
AIHLSQEEQEKFYLGFSNEIIWPLFHDLQSRCNFDPSYWTTYEQVNRKYAETIAQHYRDDTYIWVQDYQLINVACELRQL
GIDAHIGFFLHIPFPHIDIFLKLPWREQILNGLLEYDLLGFQTNRDLNNFLICVEALLPDVEIDRSGEYPLILHADRNPT
KVGFFPISIDPQEFRAAAHTPNVDNLYSQIREAFSERKIILGVDRLDYTKGIPDRLQAFSALLETYPELQQKISLTQVVV
PSREDISEYADLKQTIQQLVSEINGKYTKIGWVPIHYIFRSLPREELLAYYRAAHAALITPLKDGMNLVAKEYAVCGPDD
GVLILSEFAGAAMQLSEHSLTVNPYDINGTAQALYNAVTMPRLERIEHISKLRHSINQYDIYWWVDTFLQAANVKRSGAI
EQGSSL

Sequences:

>Translated_486_residues
MQELPNRLMIVSNRLPISIAEQADGTPTIKPASGGLVTALDPILRQARGTWIGWSGTNEADQTVDQLLEHAANSIGYSMH
AIHLSQEEQEKFYLGFSNEIIWPLFHDLQSRCNFDPSYWTTYEQVNRKYAETIAQHYRDDTYIWVQDYQLINVACELRQL
GIDAHIGFFLHIPFPHIDIFLKLPWREQILNGLLEYDLLGFQTNRDLNNFLICVEALLPDVEIDRSGEYPLILHADRNPT
KVGFFPISIDPQEFRAAAHTPNVDNLYSQIREAFSERKIILGVDRLDYTKGIPDRLQAFSALLETYPELQQKISLTQVVV
PSREDISEYADLKQTIQQLVSEINGKYTKIGWVPIHYIFRSLPREELLAYYRAAHAALITPLKDGMNLVAKEYAVCGPDD
GVLILSEFAGAAMQLSEHSLTVNPYDINGTAQALYNAVTMPRLERIEHISKLRHSINQYDIYWWVDTFLQAANVKRSGAI
EQGSSL
>Mature_486_residues
MQELPNRLMIVSNRLPISIAEQADGTPTIKPASGGLVTALDPILRQARGTWIGWSGTNEADQTVDQLLEHAANSIGYSMH
AIHLSQEEQEKFYLGFSNEIIWPLFHDLQSRCNFDPSYWTTYEQVNRKYAETIAQHYRDDTYIWVQDYQLINVACELRQL
GIDAHIGFFLHIPFPHIDIFLKLPWREQILNGLLEYDLLGFQTNRDLNNFLICVEALLPDVEIDRSGEYPLILHADRNPT
KVGFFPISIDPQEFRAAAHTPNVDNLYSQIREAFSERKIILGVDRLDYTKGIPDRLQAFSALLETYPELQQKISLTQVVV
PSREDISEYADLKQTIQQLVSEINGKYTKIGWVPIHYIFRSLPREELLAYYRAAHAALITPLKDGMNLVAKEYAVCGPDD
GVLILSEFAGAAMQLSEHSLTVNPYDINGTAQALYNAVTMPRLERIEHISKLRHSINQYDIYWWVDTFLQAANVKRSGAI
EQGSSL

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=467, Percent_Identity=37.6873661670236, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI115533558, Length=551, Percent_Identity=29.2196007259528, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI115533560, Length=551, Percent_Identity=29.2196007259528, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI71987012, Length=550, Percent_Identity=27.4545454545455, Blast_Score=195, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6319602, Length=475, Percent_Identity=37.2631578947368, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6323537, Length=467, Percent_Identity=29.3361884368308, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320279, Length=412, Percent_Identity=29.126213592233, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323917, Length=474, Percent_Identity=26.7932489451477, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI19920676, Length=505, Percent_Identity=37.6237623762376, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 55452; Mature: 55452

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELPNRLMIVSNRLPISIAEQADGTPTIKPASGGLVTALDPILRQARGTWIGWSGTNEA
CCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHH
DQTVDQLLEHAANSIGYSMHAIHLSQEEQEKFYLGFSNEIIWPLFHDLQSRCNFDPSYWT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHH
TYEQVNRKYAETIAQHYRDDTYIWVQDYQLINVACELRQLGIDAHIGFFLHIPFPHIDIF
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEE
LKLPWREQILNGLLEYDLLGFQTNRDLNNFLICVEALLPDVEIDRSGEYPLILHADRNPT
EECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEECCCCCC
KVGFFPISIDPQEFRAAAHTPNVDNLYSQIREAFSERKIILGVDRLDYTKGIPDRLQAFS
EEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECHHHHCCCCHHHHHHHH
ALLETYPELQQKISLTQVVVPSREDISEYADLKQTIQQLVSEINGKYTKIGWVPIHYIFR
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
SLPREELLAYYRAAHAALITPLKDGMNLVAKEYAVCGPDDGVLILSEFAGAAMQLSEHSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHEECCCEE
TVNPYDINGTAQALYNAVTMPRLERIEHISKLRHSINQYDIYWWVDTFLQAANVKRSGAI
EECCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCC
EQGSSL
CCCCCC
>Mature Secondary Structure
MQELPNRLMIVSNRLPISIAEQADGTPTIKPASGGLVTALDPILRQARGTWIGWSGTNEA
CCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHH
DQTVDQLLEHAANSIGYSMHAIHLSQEEQEKFYLGFSNEIIWPLFHDLQSRCNFDPSYWT
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHH
TYEQVNRKYAETIAQHYRDDTYIWVQDYQLINVACELRQLGIDAHIGFFLHIPFPHIDIF
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEE
LKLPWREQILNGLLEYDLLGFQTNRDLNNFLICVEALLPDVEIDRSGEYPLILHADRNPT
EECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEECCCCCC
KVGFFPISIDPQEFRAAAHTPNVDNLYSQIREAFSERKIILGVDRLDYTKGIPDRLQAFS
EEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECHHHHCCCCHHHHHHHH
ALLETYPELQQKISLTQVVVPSREDISEYADLKQTIQQLVSEINGKYTKIGWVPIHYIFR
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHH
SLPREELLAYYRAAHAALITPLKDGMNLVAKEYAVCGPDDGVLILSEFAGAAMQLSEHSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCHHHEECCCEE
TVNPYDINGTAQALYNAVTMPRLERIEHISKLRHSINQYDIYWWVDTFLQAANVKRSGAI
EECCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCC
EQGSSL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA