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The map label for this gene is otsB [H]

Identifier: 159899346

GI number: 159899346

Start: 3594397

End: 3595158

Strand: Reverse

Name: otsB [H]

Synonym: Haur_2827

Alternate gene names: 159899346

Gene position: 3595158-3594397 (Counterclockwise)

Preceding gene: 159899347

Following gene: 159899344

Centisome position: 56.65

GC content: 55.38

Gene sequence:

>762_bases
TTGGGATTAATCACCGATATTGATGGTACGATCAGTCGAATTGCACCAACGCCTGATGGTGCAACCGTTGATCCTTTATG
TCGGGCCGCTTTGAGCCAATTGACTGAGCATTTACCCCTCGTTGCCGCAGTTTCTGGTCGTGCCGCCCGCGATGTGCAAC
GTATGCTGCAACTGCCAGCGATGCGCTACATCGGCAACCATGGCTTGGAAATCTGGGGCCAAGATGGTGGGGTACTCGTG
CCCGCTGCGCAACCATATAGCGCCGCCGTGCGCGAATTTGTGGCAGCGATGCAGCGCTACGAGCTACCTGAAGGCGTTGT
GCTTGAATCCAAGGGCATCACGGCCACCTTACATTATCGCTTGGCGACTGATCAAACGGCGGCTGAGCTTTGGCTGCGCA
AGGTTTTGAGCGAACTAGCCGCCGCCCACAATTTGATCATCACCGAAGGGTTGATGATTTTCGAGGTGCGCCCACCCGTT
GATTGGCATAAGGGCAGCGCGGTAGCCTGGCTGATCGAGGATCACAAGCTTGAGGCAGCCATCTTTTTGGGCGACGATCT
GACCGATGTCGATGGCTTTCGCTCGATTGCGGCAGCGCGTGAACGCGGTTGCCAAGCCTTGGCGATCGGTGTGATCAATC
CCGAATCGCATCCATCGGTGGCCGAAACTGCCGATATCTTGATCAACGGCGTTGATGCAAGTGCTGAAGTTTTACAATGG
ATTCTTGCACAGCGGTTGAGCGATAGCCATGGCGTTGATTAG

Upstream 100 bases:

>100_bases
CTAGCGCCGCATGGCTTTATCTGGTTCCGAATCGAAGGAGTGCGCCTTGCTCTCGACGACGCTTAAACAACGGTTGCAGC
CAATTATCAAAGCGGAGCGC

Downstream 100 bases:

>100_bases
CTATTGTTATCAATGCTAATCAAGCATATGTGTTCGAATGAAGCGCATCGATAAATGGAAGGCTAACCGATGCGCTTCGT
TCATTTAATAAGCTGGCGGT

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGLITDIDGTISRIAPTPDGATVDPLCRAALSQLTEHLPLVAAVSGRAARDVQRMLQLPAMRYIGNHGLEIWGQDGGVLV
PAAQPYSAAVREFVAAMQRYELPEGVVLESKGITATLHYRLATDQTAAELWLRKVLSELAAAHNLIITEGLMIFEVRPPV
DWHKGSAVAWLIEDHKLEAAIFLGDDLTDVDGFRSIAAARERGCQALAIGVINPESHPSVAETADILINGVDASAEVLQW
ILAQRLSDSHGVD

Sequences:

>Translated_253_residues
MGLITDIDGTISRIAPTPDGATVDPLCRAALSQLTEHLPLVAAVSGRAARDVQRMLQLPAMRYIGNHGLEIWGQDGGVLV
PAAQPYSAAVREFVAAMQRYELPEGVVLESKGITATLHYRLATDQTAAELWLRKVLSELAAAHNLIITEGLMIFEVRPPV
DWHKGSAVAWLIEDHKLEAAIFLGDDLTDVDGFRSIAAARERGCQALAIGVINPESHPSVAETADILINGVDASAEVLQW
ILAQRLSDSHGVD
>Mature_252_residues
GLITDIDGTISRIAPTPDGATVDPLCRAALSQLTEHLPLVAAVSGRAARDVQRMLQLPAMRYIGNHGLEIWGQDGGVLVP
AAQPYSAAVREFVAAMQRYELPEGVVLESKGITATLHYRLATDQTAAELWLRKVLSELAAAHNLIITEGLMIFEVRPPVD
WHKGSAVAWLIEDHKLEAAIFLGDDLTDVDGFRSIAAARERGCQALAIGVINPESHPSVAETADILINGVDASAEVLQWI
LAQRLSDSHGVD

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Drosophila melanogaster, GI19920676, Length=201, Percent_Identity=34.3283582089552, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221473368, Length=207, Percent_Identity=28.5024154589372, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129307, Length=207, Percent_Identity=28.5024154589372, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582490, Length=207, Percent_Identity=28.5024154589372, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129309, Length=209, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27220; Mature: 27089

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLITDIDGTISRIAPTPDGATVDPLCRAALSQLTEHLPLVAAVSGRAARDVQRMLQLPA
CCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
MRYIGNHGLEIWGQDGGVLVPAAQPYSAAVREFVAAMQRYELPEGVVLESKGITATLHYR
HHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEE
LATDQTAAELWLRKVLSELAAAHNLIITEGLMIFEVRPPVDWHKGSAVAWLIEDHKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCEEEEEEECCCEEEE
IFLGDDLTDVDGFRSIAAARERGCQALAIGVINPESHPSVAETADILINGVDASAEVLQW
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHEEECCCCHHHHHHH
ILAQRLSDSHGVD
HHHHHHCCCCCCC
>Mature Secondary Structure 
GLITDIDGTISRIAPTPDGATVDPLCRAALSQLTEHLPLVAAVSGRAARDVQRMLQLPA
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
MRYIGNHGLEIWGQDGGVLVPAAQPYSAAVREFVAAMQRYELPEGVVLESKGITATLHYR
HHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEE
LATDQTAAELWLRKVLSELAAAHNLIITEGLMIFEVRPPVDWHKGSAVAWLIEDHKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCEEEEEEECCCEEEE
IFLGDDLTDVDGFRSIAAARERGCQALAIGVINPESHPSVAETADILINGVDASAEVLQW
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHEEECCCCHHHHHHH
ILAQRLSDSHGVD
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]