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The map label for this gene is trxB [C]

Identifier: 159899341

GI number: 159899341

Start: 3589640

End: 3590734

Strand: Reverse

Name: trxB [C]

Synonym: Haur_2822

Alternate gene names: 159899341

Gene position: 3590734-3589640 (Counterclockwise)

Preceding gene: 159899342

Following gene: 159899340

Centisome position: 56.58

GC content: 52.15

Gene sequence:

>1095_bases
ATGGCTAATCAAACGATTGTGATTGGGGCTGGTCAGGCTGGCTTGGCGGCTGGCTATTGGCTGCAACAAGCTAAAATTCC
ATTTCAAATTATCGAGGCTCAAGCCAGCGTTGGTGGCTCGTGGCCCGCCTATTACGATAGTTTGAGCTTGTTTTCGCCTG
CGCGATTTTCAAGTTTGCCAGGCATGGCTTTCCCTGCGCCCGCCGATAGCTACCCGCAGCGCGATACTGTTGTGGCCTAT
TTGCAGCGCTATGCTGAACATTTCAATTTACCGATTCAGACGAATACGGCAATTAGTACGATCGAACCGCAGAATGGTGG
CTTTCGCCTGACAAGTAGTGCGGGACAAGTATTTCATGCGGGTCAAATTATTGCCGCAACTGGAGCGTTTGCGCGGCCAT
TTATGCCCGAATTGCCCAACCAAGCTGCATTTCAAGGCAAGATTTTACATAGTGCTCGCTATCGCGATTCAGCAGATTTT
GTGGGCAAACGGGTTGTGGTGGTTGGCGCAGGCAATTCGGCAATTCAAATTGCGATCGAGCTGGCCCAAGTTGCCGATGT
GACCCTAGCTACGCGCCAGCCAATTCGCTTTCAAGCCCAACGGATTGCAGGCCGTGATATCCATTGGTGGTGGTGGCTGA
CAGGTTTTGATCGGCTGGCACTCAACACGCGAGTTGGCCGTTGGATTCAGCAACGAACCCAAGGAATTGTCCTCGATACT
GGTCTGTACAGCAGAAAAATCAATCAAAACCAACCCCAACGCCGAGCCATGTTCGAACGTTTTAGCGCAACGGGTGTAGT
TTGGGCTGATGGACAGCCCGAAGCAGTTGACATAGTATTGTTTGCGACTGGCTATCGACCTCATTTGAGCTATTTGCAAG
GCCTCAATGCGCTTGATCAAGCTGGCTTGCCCTTGCATCGGGCAGGTGTGAGCACCACAGTTGAGGGCTTGTATTACGTT
GGCTTGGAGCAACAAACTAATTTTGCCTCGGCTACGTTGCGTGGAGTTGGGCCGGATGCATCGCGAGTGGTGCGCCATAT
TCAAGTTCGCATGCAGCAACCAGCAGGTTGTTGTCGCTGGGCGATGGGGCAATGA

Upstream 100 bases:

>100_bases
TTGGTGGGATTGTTGCATAGCTATCAACCGCTGTTTGGCTTGCTGTTGCTGATGTGTTTGATCAGCGCAGCGGCAATTTA
TTTGATTCGAGGTGCAAGTA

Downstream 100 bases:

>100_bases
ACCAGCTTGATCTGACTAACCTTTGGCAAAACCTAGCGCAGCCATTGCGGCGATTTTTGCAGGCTCGTTTAGCCGATCAA
GCAGCAGTCGAAGATTTGTT

Product: putative flavin-binding monooxygenase involved in arsenic resistance

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MANQTIVIGAGQAGLAAGYWLQQAKIPFQIIEAQASVGGSWPAYYDSLSLFSPARFSSLPGMAFPAPADSYPQRDTVVAY
LQRYAEHFNLPIQTNTAISTIEPQNGGFRLTSSAGQVFHAGQIIAATGAFARPFMPELPNQAAFQGKILHSARYRDSADF
VGKRVVVVGAGNSAIQIAIELAQVADVTLATRQPIRFQAQRIAGRDIHWWWWLTGFDRLALNTRVGRWIQQRTQGIVLDT
GLYSRKINQNQPQRRAMFERFSATGVVWADGQPEAVDIVLFATGYRPHLSYLQGLNALDQAGLPLHRAGVSTTVEGLYYV
GLEQQTNFASATLRGVGPDASRVVRHIQVRMQQPAGCCRWAMGQ

Sequences:

>Translated_364_residues
MANQTIVIGAGQAGLAAGYWLQQAKIPFQIIEAQASVGGSWPAYYDSLSLFSPARFSSLPGMAFPAPADSYPQRDTVVAY
LQRYAEHFNLPIQTNTAISTIEPQNGGFRLTSSAGQVFHAGQIIAATGAFARPFMPELPNQAAFQGKILHSARYRDSADF
VGKRVVVVGAGNSAIQIAIELAQVADVTLATRQPIRFQAQRIAGRDIHWWWWLTGFDRLALNTRVGRWIQQRTQGIVLDT
GLYSRKINQNQPQRRAMFERFSATGVVWADGQPEAVDIVLFATGYRPHLSYLQGLNALDQAGLPLHRAGVSTTVEGLYYV
GLEQQTNFASATLRGVGPDASRVVRHIQVRMQQPAGCCRWAMGQ
>Mature_363_residues
ANQTIVIGAGQAGLAAGYWLQQAKIPFQIIEAQASVGGSWPAYYDSLSLFSPARFSSLPGMAFPAPADSYPQRDTVVAYL
QRYAEHFNLPIQTNTAISTIEPQNGGFRLTSSAGQVFHAGQIIAATGAFARPFMPELPNQAAFQGKILHSARYRDSADFV
GKRVVVVGAGNSAIQIAIELAQVADVTLATRQPIRFQAQRIAGRDIHWWWWLTGFDRLALNTRVGRWIQQRTQGIVLDTG
LYSRKINQNQPQRRAMFERFSATGVVWADGQPEAVDIVLFATGYRPHLSYLQGLNALDQAGLPLHRAGVSTTVEGLYYVG
LEQQTNFASATLRGVGPDASRVVRHIQVRMQQPAGCCRWAMGQ

Specific function: Unknown

COG id: COG2072

COG function: function code P; Predicted flavoprotein involved in K+ transport

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4503759, Length=338, Percent_Identity=28.1065088757396, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4503755, Length=417, Percent_Identity=23.021582733813, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI4503757, Length=344, Percent_Identity=24.1279069767442, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI50541965, Length=333, Percent_Identity=24.024024024024, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI50541961, Length=333, Percent_Identity=24.024024024024, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221316674, Length=375, Percent_Identity=23.7333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221316672, Length=375, Percent_Identity=23.7333333333333, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25145785, Length=204, Percent_Identity=31.8627450980392, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561948, Length=348, Percent_Identity=26.7241379310345, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17555726, Length=362, Percent_Identity=24.8618784530387, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193202226, Length=208, Percent_Identity=28.3653846153846, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17506045, Length=178, Percent_Identity=26.9662921348315, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25150462, Length=339, Percent_Identity=25.6637168141593, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922866, Length=343, Percent_Identity=21.865889212828, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR000103 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 39933; Mature: 39802

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQTIVIGAGQAGLAAGYWLQQAKIPFQIIEAQASVGGSWPAYYDSLSLFSPARFSSLP
CCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHCCHHHCCCHHHCCCC
GMAFPAPADSYPQRDTVVAYLQRYAEHFNLPIQTNTAISTIEPQNGGFRLTSSAGQVFHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCEEEECCCCCEEEC
GQIIAATGAFARPFMPELPNQAAFQGKILHSARYRDSADFVGKRVVVVGAGNSAIQIAIE
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCEEEEEEE
LAQVADVTLATRQPIRFQAQRIAGRDIHWWWWLTGFDRLALNTRVGRWIQQRTQGIVLDT
HHHHHHHHECCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEC
GLYSRKINQNQPQRRAMFERFSATGVVWADGQPEAVDIVLFATGYRPHLSYLQGLNALDQ
CHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCHHHH
AGLPLHRAGVSTTVEGLYYVGLEQQTNFASATLRGVGPDASRVVRHIQVRMQQPAGCCRW
CCCCEECCCCCCEECEEEEEEECCCCCHHHEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
AMGQ
CCCC
>Mature Secondary Structure 
ANQTIVIGAGQAGLAAGYWLQQAKIPFQIIEAQASVGGSWPAYYDSLSLFSPARFSSLP
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHCCHHHCCCHHHCCCC
GMAFPAPADSYPQRDTVVAYLQRYAEHFNLPIQTNTAISTIEPQNGGFRLTSSAGQVFHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCEEEECCCCCEEEC
GQIIAATGAFARPFMPELPNQAAFQGKILHSARYRDSADFVGKRVVVVGAGNSAIQIAIE
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCEEEEEEE
LAQVADVTLATRQPIRFQAQRIAGRDIHWWWWLTGFDRLALNTRVGRWIQQRTQGIVLDT
HHHHHHHHECCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEC
GLYSRKINQNQPQRRAMFERFSATGVVWADGQPEAVDIVLFATGYRPHLSYLQGLNALDQ
CHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCCCHHHH
AGLPLHRAGVSTTVEGLYYVGLEQQTNFASATLRGVGPDASRVVRHIQVRMQQPAGCCRW
CCCCEECCCCCCEECEEEEEEECCCCCHHHEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
AMGQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA