Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 159899304

GI number: 159899304

Start: 3541491

End: 3543215

Strand: Reverse

Name: metG [H]

Synonym: Haur_2785

Alternate gene names: 159899304

Gene position: 3543215-3541491 (Counterclockwise)

Preceding gene: 159899305

Following gene: 159899303

Centisome position: 55.83

GC content: 50.78

Gene sequence:

>1725_bases
ATGGCGAAAAATATTTTAGTGGCGGTCGCGTGGCCCTATGCTTCCGGCGCTCGACACCTTGGCCATGTTGCTGGCTTTGG
CGTACCAAGCGATGTATTTGCCCGCTATCAACGCTTGGTTGGAAACAATGTGCTCATGGTCAGTGGCACTGACGATCATG
GCACGCCGATCACGGTGCGAGCCGATCGCGAGGGCAAAACACCCCGCGAAGTAACCGATTTCTACAATGCTGAAATTCGC
AACAATTTGCGTGATTTGGGCTTGTCGTATGATTTGTTTACCCGCACATCAACCGAAAACCATTACCAAATAACCCAAGC
ATTTTTTACCCGCTTGCAAGAAAAAGGTTATATTTTTGCTCAAGAAATGATTGGAACCTACAGCGAAGCTGACAAGCGCT
TTTTGCCCGACCGCTACGTTGAAGGCACATGCCCGCATTGTGGCTACACCAAAGCCCGTGGCGATCAGTGCGATAACTGT
GGTAAGCAGCTTGATCCGGTTGATTTGATCGAGCCACGTTCGACCTTGAGTGGCGCAACGCCGGTGTTCAAGCCAACAAC
CCACTTTTTCTTAAATTTGCCAGCCTTTGTTGAACGTTTGCGCGAATGGATCGAAAGCCAAAACCATTGGCGACCAAATG
TTAAGCGCTTCTCGTTGGGCTTACTCGATGATGTACCAGCTCGCGCGATCACCCGCGACCTGACGTGGGGCGTGCCAATT
CCGGTGGAAGGCGACGAATTCGATAGCAAGCGAATTTATGTGTGGTTTGATGCAGTGATTGGCTATCTTTCAGCAGCAGT
TGAATGGTCGATCAAGGTTGGGCGGCCTGAGGCTTGGCGCGATTGGTGGCTCAACTCCGACGCGCGGCATTATTATTTCA
TGGGCAAAGATAATATTGTGTTTCATAGCGTGATTTGGCCCGCCATGCTGATTGGTCATGGCGAATTGGAATTGCCCTAT
GAGGTCGTCAGTAGCGAGTTTTTGACCTTGGCAGGCGGCGAGAAAATCTCATCATCACGCGCTGAAGGCAATAGTATTCC
GTTTGTTGGCGAATTTTTGGCCCAGTATGATCCCGATCCGTTGCGCTATTTCTTGGTGATTGCTGGCCCTGAAACCTCGG
ATACTGAGTGGTCGCTGGGCGAATTCATTCGACGCAACAACGAAGAGTTAGTAGCAACCTGGGGTAATTTGGTCAATCGG
GTACTTTCAATCACCCACAAAAATTTTGGCCATGTGCCAACGCCGCAAGCCCTCACCGCCGAGGATGAAGCCGCCTTGGC
GACTGTGCGGGCGGCTTTTGAAACGGTTGGCAAGGAATTAGAGCAAGCTCATTTCAAAGCTGGCTTGATCGCCACTATGG
CAGCAGCGCGTTCGGTCAACGAATATTTGGCCAGCCAAGAACCATGGAAGGTGCTCAAAACCGATCGCGAACGCGGTGGC
ACGATTTTGTATGTTGCCTTGCAAGCGATTAATAATTTGCGCACCTTGTTTACGCCATTTTTGCCCTTCACCAGCCAAAA
ATTGCATGAACTTTTGGGTAATGAAGGTTATTTGGCAGGGCCATTGCTGTTTGAAGAACGCCAAGAAAGCCAGCATAGCC
ACGAAGTGCTTACCTGCGAACCTAGTACTTGGATTGGCCGCTGGGAATGGAGCGATCTGCCCGCAGGTCGTGAGTTGGCC
GCCCCAAGCGTGCTGTTTAAAAAGCTCGAATTACCAACCGAGTAG

Upstream 100 bases:

>100_bases
GCCAACTAGAAGATGCGCCCACCTCGTTGATTCGTACAACCGGGCGACTTGCGCTCGGTTTTTTGTTGCCCTCAATGATT
GTTTTTTTAAGGATGTGTGT

Downstream 100 bases:

>100_bases
GTTCATATAGAACAACGATTTAACCACGAAGGACACGAAGAACCACTACGGTTAATAACTTCGTGTCCTTCGCGATCTTT
GCGGTTAAAAAATGGATCAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MAKNILVAVAWPYASGARHLGHVAGFGVPSDVFARYQRLVGNNVLMVSGTDDHGTPITVRADREGKTPREVTDFYNAEIR
NNLRDLGLSYDLFTRTSTENHYQITQAFFTRLQEKGYIFAQEMIGTYSEADKRFLPDRYVEGTCPHCGYTKARGDQCDNC
GKQLDPVDLIEPRSTLSGATPVFKPTTHFFLNLPAFVERLREWIESQNHWRPNVKRFSLGLLDDVPARAITRDLTWGVPI
PVEGDEFDSKRIYVWFDAVIGYLSAAVEWSIKVGRPEAWRDWWLNSDARHYYFMGKDNIVFHSVIWPAMLIGHGELELPY
EVVSSEFLTLAGGEKISSSRAEGNSIPFVGEFLAQYDPDPLRYFLVIAGPETSDTEWSLGEFIRRNNEELVATWGNLVNR
VLSITHKNFGHVPTPQALTAEDEAALATVRAAFETVGKELEQAHFKAGLIATMAAARSVNEYLASQEPWKVLKTDRERGG
TILYVALQAINNLRTLFTPFLPFTSQKLHELLGNEGYLAGPLLFEERQESQHSHEVLTCEPSTWIGRWEWSDLPAGRELA
APSVLFKKLELPTE

Sequences:

>Translated_574_residues
MAKNILVAVAWPYASGARHLGHVAGFGVPSDVFARYQRLVGNNVLMVSGTDDHGTPITVRADREGKTPREVTDFYNAEIR
NNLRDLGLSYDLFTRTSTENHYQITQAFFTRLQEKGYIFAQEMIGTYSEADKRFLPDRYVEGTCPHCGYTKARGDQCDNC
GKQLDPVDLIEPRSTLSGATPVFKPTTHFFLNLPAFVERLREWIESQNHWRPNVKRFSLGLLDDVPARAITRDLTWGVPI
PVEGDEFDSKRIYVWFDAVIGYLSAAVEWSIKVGRPEAWRDWWLNSDARHYYFMGKDNIVFHSVIWPAMLIGHGELELPY
EVVSSEFLTLAGGEKISSSRAEGNSIPFVGEFLAQYDPDPLRYFLVIAGPETSDTEWSLGEFIRRNNEELVATWGNLVNR
VLSITHKNFGHVPTPQALTAEDEAALATVRAAFETVGKELEQAHFKAGLIATMAAARSVNEYLASQEPWKVLKTDRERGG
TILYVALQAINNLRTLFTPFLPFTSQKLHELLGNEGYLAGPLLFEERQESQHSHEVLTCEPSTWIGRWEWSDLPAGRELA
APSVLFKKLELPTE
>Mature_573_residues
AKNILVAVAWPYASGARHLGHVAGFGVPSDVFARYQRLVGNNVLMVSGTDDHGTPITVRADREGKTPREVTDFYNAEIRN
NLRDLGLSYDLFTRTSTENHYQITQAFFTRLQEKGYIFAQEMIGTYSEADKRFLPDRYVEGTCPHCGYTKARGDQCDNCG
KQLDPVDLIEPRSTLSGATPVFKPTTHFFLNLPAFVERLREWIESQNHWRPNVKRFSLGLLDDVPARAITRDLTWGVPIP
VEGDEFDSKRIYVWFDAVIGYLSAAVEWSIKVGRPEAWRDWWLNSDARHYYFMGKDNIVFHSVIWPAMLIGHGELELPYE
VVSSEFLTLAGGEKISSSRAEGNSIPFVGEFLAQYDPDPLRYFLVIAGPETSDTEWSLGEFIRRNNEELVATWGNLVNRV
LSITHKNFGHVPTPQALTAEDEAALATVRAAFETVGKELEQAHFKAGLIATMAAARSVNEYLASQEPWKVLKTDRERGGT
ILYVALQAINNLRTLFTPFLPFTSQKLHELLGNEGYLAGPLLFEERQESQHSHEVLTCEPSTWIGRWEWSDLPAGRELAA
PSVLFKKLELPTE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=582, Percent_Identity=34.3642611683849, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI24308436, Length=529, Percent_Identity=27.2211720226843, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1788432, Length=521, Percent_Identity=33.2053742802303, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1786861, Length=208, Percent_Identity=27.8846153846154, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17540794, Length=586, Percent_Identity=33.1058020477816, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=26.2135922330097, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=416, Percent_Identity=24.5192307692308, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=524, Percent_Identity=34.7328244274809, Blast_Score=320, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6321610, Length=541, Percent_Identity=24.2144177449168, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24655311, Length=587, Percent_Identity=34.7529812606474, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI21358483, Length=526, Percent_Identity=27.5665399239544, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355409, Length=139, Percent_Identity=32.3741007194245, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 64850; Mature: 64719

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNILVAVAWPYASGARHLGHVAGFGVPSDVFARYQRLVGNNVLMVSGTDDHGTPITVR
CCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
ADREGKTPREVTDFYNAEIRNNLRDLGLSYDLFTRTSTENHYQITQAFFTRLQEKGYIFA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEH
QEMIGTYSEADKRFLPDRYVEGTCPHCGYTKARGDQCDNCGKQLDPVDLIEPRSTLSGAT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHCCCCC
PVFKPTTHFFLNLPAFVERLREWIESQNHWRPNVKRFSLGLLDDVPARAITRDLTWGVPI
CCCCCCHHHEEECHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHCCCCCCCC
PVEGDEFDSKRIYVWFDAVIGYLSAAVEWSIKVGRPEAWRDWWLNSDARHYYFMGKDNIV
CCCCCCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCEEEEECCCCEE
FHSVIWPAMLIGHGELELPYEVVSSEFLTLAGGEKISSSRAEGNSIPFVGEFLAQYDPDP
EHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCCCHHCCCCCCCCCCCHHHHHHHHCCCCC
LRYFLVIAGPETSDTEWSLGEFIRRNNEELVATWGNLVNRVLSITHKNFGHVPTPQALTA
EEEEEEEECCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EDEAALATVRAAFETVGKELEQAHFKAGLIATMAAARSVNEYLASQEPWKVLKTDRERGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
TILYVALQAINNLRTLFTPFLPFTSQKLHELLGNEGYLAGPLLFEERQESQHSHEVLTCE
EEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEEEC
PSTWIGRWEWSDLPAGRELAAPSVLFKKLELPTE
CCCCCCCEECCCCCCCCHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AKNILVAVAWPYASGARHLGHVAGFGVPSDVFARYQRLVGNNVLMVSGTDDHGTPITVR
CCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
ADREGKTPREVTDFYNAEIRNNLRDLGLSYDLFTRTSTENHYQITQAFFTRLQEKGYIFA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEH
QEMIGTYSEADKRFLPDRYVEGTCPHCGYTKARGDQCDNCGKQLDPVDLIEPRSTLSGAT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHCCCCC
PVFKPTTHFFLNLPAFVERLREWIESQNHWRPNVKRFSLGLLDDVPARAITRDLTWGVPI
CCCCCCHHHEEECHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHCCCCCCCC
PVEGDEFDSKRIYVWFDAVIGYLSAAVEWSIKVGRPEAWRDWWLNSDARHYYFMGKDNIV
CCCCCCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCEEEEECCCCEE
FHSVIWPAMLIGHGELELPYEVVSSEFLTLAGGEKISSSRAEGNSIPFVGEFLAQYDPDP
EHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCCCHHCCCCCCCCCCCHHHHHHHHCCCCC
LRYFLVIAGPETSDTEWSLGEFIRRNNEELVATWGNLVNRVLSITHKNFGHVPTPQALTA
EEEEEEEECCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EDEAALATVRAAFETVGKELEQAHFKAGLIATMAAARSVNEYLASQEPWKVLKTDRERGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
TILYVALQAINNLRTLFTPFLPFTSQKLHELLGNEGYLAGPLLFEERQESQHSHEVLTCE
EEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEEEC
PSTWIGRWEWSDLPAGRELAAPSVLFKKLELPTE
CCCCCCCEECCCCCCCCHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA