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The map label for this gene is metE

Identifier: 159899092

GI number: 159899092

Start: 3297559

End: 3299805

Strand: Reverse

Name: metE

Synonym: Haur_2573

Alternate gene names: 159899092

Gene position: 3299805-3297559 (Counterclockwise)

Preceding gene: 159899093

Following gene: 159899091

Centisome position: 51.99

GC content: 51.0

Gene sequence:

>2247_bases
ATGAATTTTCAAACAACAGTTGTGGGTTATCCACGGGTTGGCGTTGGCCGGCCATACAAGCAGGCTTTGGAACGCTTTTG
GAGTGGCAAACTCGATGAAACTGGTTTTCGGGCGGCGATCAACGAATTATGCCACGATCGGTTGGCGACCCAAGCCCAGC
GCTTGGATCTTGTGCCCGTTGGCGATTTTAGTTTGTACGACCATGTATTGGATACGGCGCTGATGTTGGGAGCTGTGCCA
GCACGCTTTGGCAAGGTCGATGCGCATAATTTACAGGGCTATTTTGCACTGGCGCGAGGTCGTGATGGCCTGCCAGCCTT
GGAAATGACCAAATGGTTTGATACAAACTATCATTATTTAGTGCCGGAAATTCCTGAGCGTTGGGAACTGCAAGCCAATT
TGGTGCTGGAACAAGTGCGTTTTGCCCAGAATGTGGTTGGAGCAAAAGCTCGCCCGGTGTTGCTTGGGCCTTGGACATTC
TTGCGCTTGGCTCGCTTGGCGGGAGCCGAATTAGCCCAACATCTACAACAATTAACTGCAATGTATGCCCAAATTGTGCG
CGAATTGAATGATTGTAATGTAGCATTTATTCAATGTGACGAGCCTGCTTTGGTTGGCGATGTGACTGAAGAAGAATGGC
AAGCCTTTGCCGCCTGCTATCGCGAGCTGAGCAAGCACGGCAAGATCGTGGTGCAAACCTATTATGGTGATGTTACGCCG
TGGTATCGCGAACTGTGTCGCTTACCAATCCATGGCTTAGGCTTGGATTTGGTGCAAGGCCACGCCAATTGGGCTGCGAT
TCAATATCATGGCTTTCCGCAGGACAAAATTTTAGTCGCTGGGGTGGTTAATGGGCGCAACGTTTGGCGGAGCGATTTAG
CCGATTTGTATACCAAAATTACTGGATTAAGCGAATTTGTCGCACCCGAACGCTTGATCTTGAGCAGCTCATGTTCATTG
TTACACCTGCCCGAAACCGTAACTGCTGAACGTAATTTGCCTGTTGCGGTTAGCAGTGGTTTGGCCTTTGCCCAAGAACG
TTTGGCCGAACTAGAATTATTAGCTAACGCCTTACGCGATGGCATTGCAAGCGTGCAACCAACTTGGGATGCAGCGCTGG
CCTCGCGTCAGCAATGGCTTGATGGCGTTGGCCGAATTGTGCCAGCGGTACGTGAGCGCACTGCTGCCTTGGGTACTGAA
ACACCAGCACGTTTGGCCTATGCTGAACGTGTGCCATTGCAACAAGCCAAATTGAACTTGCCACTGTTGCCAACCACCAC
AATTGGCTCGTTCCCGCAAACTGCGGCTTTGCGCAAAGCTCGCGCTGAAGCCAAGCGCAACCCAACTGGCTATGCCGAAA
CAATCAATGCCGAAATTGCCCATGTGATTGCGCTGCAAGAACAGTGGGGAATTGATGTGTTGGTGCATGGCGAGCCAGAG
CGCAACGATATGGTGCAATTTTTCGCCGAGCATTTGGCAGGTTACGTAGCCACTCAAGAAGGTTGGGTACAAAGTTATGG
TAGTCGCTGTGTGCGCCCGCCAGTGATTGCTGGTGATGTTTATCGCACGGCGGCTTTGAGTGTTGCCGAAACTGCCTATG
CTCAATCGCTGACCAAGCGACCTGTCAAGGGCATGTTGACTGGGCCAGTGACGATGTTGCAATGGAGCTTTGTGCGCGAT
GATTTGCCGCGTAGCGCCGTTGCTGCCCAAATTGGCTTAGCCTTGCGCGATGAAGTAACTGATTTAGAGGCAGCAGGCAT
TAACATCATTCAAATTGACGAGCCTGCCTTCCGTGAGGGCTTACCGTTACGTCGCAACGATTGGCAAAACTACCTTGATT
GGGCGGTGCGAGCTTTTCGAATTGCGACCAGCGATGCCAAACCGAGCACCCAAATTCATACCCATATGTGCTATAGCGAT
TTCAACGATATTATTGCCGCAATTGCCGCGCTTGATGCTGATGTGATTAGCATTGAAGATGCCCGCAGTGCTGGCTCGCT
GTTAGCTGGCTTAGAAGGCCGCTACACCCAACAAATTGGGCCTGGCGTGTATGATATTCACAGCCCAAATATTCCAACAG
TTGAACAATTGGTTGCGCGAATGCGGCAATTGCTCAACTATTTGCCTGCCGAGCAATTGTGGATCAACCCTGATTGTGGC
TTGAAAACTCGCACCTATGCTGAAGTTGCGGCGGCATTACAGGCGATGGTTACCGCAACCAAACAAGTGCGTAACCAAAT
CAGCTAA

Upstream 100 bases:

>100_bases
CATGGATGAGTGAAACGATCGATTTGACCAAGGAGAAGAATTTCTTTGAGACGCGGGTCACTGAATATCAATCAGCGGCC
AGTCTAGAATGGGATTAAGC

Downstream 100 bases:

>100_bases
ATTAACGTATTTTTGATCAATCCGACCTGATCAACGTGTACGAACGAAGATGCTAGCCTTCGCTGGCATCTTTGGTTTTA
GGGCTATGTTGGTTAATCAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MNFQTTVVGYPRVGVGRPYKQALERFWSGKLDETGFRAAINELCHDRLATQAQRLDLVPVGDFSLYDHVLDTALMLGAVP
ARFGKVDAHNLQGYFALARGRDGLPALEMTKWFDTNYHYLVPEIPERWELQANLVLEQVRFAQNVVGAKARPVLLGPWTF
LRLARLAGAELAQHLQQLTAMYAQIVRELNDCNVAFIQCDEPALVGDVTEEEWQAFAACYRELSKHGKIVVQTYYGDVTP
WYRELCRLPIHGLGLDLVQGHANWAAIQYHGFPQDKILVAGVVNGRNVWRSDLADLYTKITGLSEFVAPERLILSSSCSL
LHLPETVTAERNLPVAVSSGLAFAQERLAELELLANALRDGIASVQPTWDAALASRQQWLDGVGRIVPAVRERTAALGTE
TPARLAYAERVPLQQAKLNLPLLPTTTIGSFPQTAALRKARAEAKRNPTGYAETINAEIAHVIALQEQWGIDVLVHGEPE
RNDMVQFFAEHLAGYVATQEGWVQSYGSRCVRPPVIAGDVYRTAALSVAETAYAQSLTKRPVKGMLTGPVTMLQWSFVRD
DLPRSAVAAQIGLALRDEVTDLEAAGINIIQIDEPAFREGLPLRRNDWQNYLDWAVRAFRIATSDAKPSTQIHTHMCYSD
FNDIIAAIAALDADVISIEDARSAGSLLAGLEGRYTQQIGPGVYDIHSPNIPTVEQLVARMRQLLNYLPAEQLWINPDCG
LKTRTYAEVAAALQAMVTATKQVRNQIS

Sequences:

>Translated_748_residues
MNFQTTVVGYPRVGVGRPYKQALERFWSGKLDETGFRAAINELCHDRLATQAQRLDLVPVGDFSLYDHVLDTALMLGAVP
ARFGKVDAHNLQGYFALARGRDGLPALEMTKWFDTNYHYLVPEIPERWELQANLVLEQVRFAQNVVGAKARPVLLGPWTF
LRLARLAGAELAQHLQQLTAMYAQIVRELNDCNVAFIQCDEPALVGDVTEEEWQAFAACYRELSKHGKIVVQTYYGDVTP
WYRELCRLPIHGLGLDLVQGHANWAAIQYHGFPQDKILVAGVVNGRNVWRSDLADLYTKITGLSEFVAPERLILSSSCSL
LHLPETVTAERNLPVAVSSGLAFAQERLAELELLANALRDGIASVQPTWDAALASRQQWLDGVGRIVPAVRERTAALGTE
TPARLAYAERVPLQQAKLNLPLLPTTTIGSFPQTAALRKARAEAKRNPTGYAETINAEIAHVIALQEQWGIDVLVHGEPE
RNDMVQFFAEHLAGYVATQEGWVQSYGSRCVRPPVIAGDVYRTAALSVAETAYAQSLTKRPVKGMLTGPVTMLQWSFVRD
DLPRSAVAAQIGLALRDEVTDLEAAGINIIQIDEPAFREGLPLRRNDWQNYLDWAVRAFRIATSDAKPSTQIHTHMCYSD
FNDIIAAIAALDADVISIEDARSAGSLLAGLEGRYTQQIGPGVYDIHSPNIPTVEQLVARMRQLLNYLPAEQLWINPDCG
LKTRTYAEVAAALQAMVTATKQVRNQIS
>Mature_748_residues
MNFQTTVVGYPRVGVGRPYKQALERFWSGKLDETGFRAAINELCHDRLATQAQRLDLVPVGDFSLYDHVLDTALMLGAVP
ARFGKVDAHNLQGYFALARGRDGLPALEMTKWFDTNYHYLVPEIPERWELQANLVLEQVRFAQNVVGAKARPVLLGPWTF
LRLARLAGAELAQHLQQLTAMYAQIVRELNDCNVAFIQCDEPALVGDVTEEEWQAFAACYRELSKHGKIVVQTYYGDVTP
WYRELCRLPIHGLGLDLVQGHANWAAIQYHGFPQDKILVAGVVNGRNVWRSDLADLYTKITGLSEFVAPERLILSSSCSL
LHLPETVTAERNLPVAVSSGLAFAQERLAELELLANALRDGIASVQPTWDAALASRQQWLDGVGRIVPAVRERTAALGTE
TPARLAYAERVPLQQAKLNLPLLPTTTIGSFPQTAALRKARAEAKRNPTGYAETINAEIAHVIALQEQWGIDVLVHGEPE
RNDMVQFFAEHLAGYVATQEGWVQSYGSRCVRPPVIAGDVYRTAALSVAETAYAQSLTKRPVKGMLTGPVTMLQWSFVRD
DLPRSAVAAQIGLALRDEVTDLEAAGINIIQIDEPAFREGLPLRRNDWQNYLDWAVRAFRIATSDAKPSTQIHTHMCYSD
FNDIIAAIAALDADVISIEDARSAGSLLAGLEGRYTQQIGPGVYDIHSPNIPTVEQLVARMRQLLNYLPAEQLWINPDCG
LKTRTYAEVAAALQAMVTATKQVRNQIS

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=47.2368421052632, Blast_Score=645, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=771, Percent_Identity=39.9481193255512, Blast_Score=549, Evalue=1e-157,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_HERA2 (A9B0A2)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545339.1
- ProteinModelPortal:   A9B0A2
- GeneID:   5734451
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_2573
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   HAUR316274:HAUR_2573-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 82813; Mature: 82813

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQTTVVGYPRVGVGRPYKQALERFWSGKLDETGFRAAINELCHDRLATQAQRLDLVPV
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
GDFSLYDHVLDTALMLGAVPARFGKVDAHNLQGYFALARGRDGLPALEMTKWFDTNYHYL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEE
VPEIPERWELQANLVLEQVRFAQNVVGAKARPVLLGPWTFLRLARLAGAELAQHLQQLTA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
MYAQIVRELNDCNVAFIQCDEPALVGDVTEEEWQAFAACYRELSKHGKIVVQTYYGDVTP
HHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
WYRELCRLPIHGLGLDLVQGHANWAAIQYHGFPQDKILVAGVVNGRNVWRSDLADLYTKI
HHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
TGLSEFVAPERLILSSSCSLLHLPETVTAERNLPVAVSSGLAFAQERLAELELLANALRD
HHHHHHHCCHHHHHHCCCCEEECCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
GIASVQPTWDAALASRQQWLDGVGRIVPAVRERTAALGTETPARLAYAERVPLQQAKLNL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCC
PLLPTTTIGSFPQTAALRKARAEAKRNPTGYAETINAEIAHVIALQEQWGIDVLVHGEPE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEHHCCCCEEEEECCCC
RNDMVQFFAEHLAGYVATQEGWVQSYGSRCVRPPVIAGDVYRTAALSVAETAYAQSLTKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHC
PVKGMLTGPVTMLQWSFVRDDLPRSAVAAQIGLALRDEVTDLEAAGINIIQIDEPAFREG
CCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCC
LPLRRNDWQNYLDWAVRAFRIATSDAKPSTQIHTHMCYSDFNDIIAAIAALDADVISIED
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
ARSAGSLLAGLEGRYTQQIGPGVYDIHSPNIPTVEQLVARMRQLLNYLPAEQLWINPDCG
HHHHHHHHHCCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
LKTRTYAEVAAALQAMVTATKQVRNQIS
CCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFQTTVVGYPRVGVGRPYKQALERFWSGKLDETGFRAAINELCHDRLATQAQRLDLVPV
CCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
GDFSLYDHVLDTALMLGAVPARFGKVDAHNLQGYFALARGRDGLPALEMTKWFDTNYHYL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEE
VPEIPERWELQANLVLEQVRFAQNVVGAKARPVLLGPWTFLRLARLAGAELAQHLQQLTA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
MYAQIVRELNDCNVAFIQCDEPALVGDVTEEEWQAFAACYRELSKHGKIVVQTYYGDVTP
HHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
WYRELCRLPIHGLGLDLVQGHANWAAIQYHGFPQDKILVAGVVNGRNVWRSDLADLYTKI
HHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
TGLSEFVAPERLILSSSCSLLHLPETVTAERNLPVAVSSGLAFAQERLAELELLANALRD
HHHHHHHCCHHHHHHCCCCEEECCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
GIASVQPTWDAALASRQQWLDGVGRIVPAVRERTAALGTETPARLAYAERVPLQQAKLNL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCC
PLLPTTTIGSFPQTAALRKARAEAKRNPTGYAETINAEIAHVIALQEQWGIDVLVHGEPE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEHHCCCCEEEEECCCC
RNDMVQFFAEHLAGYVATQEGWVQSYGSRCVRPPVIAGDVYRTAALSVAETAYAQSLTKR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHC
PVKGMLTGPVTMLQWSFVRDDLPRSAVAAQIGLALRDEVTDLEAAGINIIQIDEPAFREG
CCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCC
LPLRRNDWQNYLDWAVRAFRIATSDAKPSTQIHTHMCYSDFNDIIAAIAALDADVISIED
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
ARSAGSLLAGLEGRYTQQIGPGVYDIHSPNIPTVEQLVARMRQLLNYLPAEQLWINPDCG
HHHHHHHHHCCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
LKTRTYAEVAAALQAMVTATKQVRNQIS
CCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA