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The map label for this gene is gabT [C]

Identifier: 159899088

GI number: 159899088

Start: 3293614

End: 3294942

Strand: Reverse

Name: gabT [C]

Synonym: Haur_2569

Alternate gene names: 159899088

Gene position: 3294942-3293614 (Counterclockwise)

Preceding gene: 159899089

Following gene: 159899086

Centisome position: 51.92

GC content: 51.32

Gene sequence:

>1329_bases
ATGTTACCCAAAATTGTGAGTGCTGTGCCTGGGCCACGTAGCCAAGCGCTCTTAGCGCAATTGGCATCCAGTGAAGCACC
GAGTTTAACCTTGCCTGGCGGCATTGTATGGGCCGAGGCCGAGGGTGCGCTGGTGACTGATGTTGATGGCAATCGCTATC
TTGATTTTGCGGCAGCCTTTGGGGTGGTCGGGATTGGCCATCGCCATCCAGCGGTGTTGGCGGCGATTCAAGCTCAAAGC
CAGCGCTTGATTCACGGAATGGGCGATGTGTTTGCCCATGAAGCGCGGATCGAGTTGGTTCAATTAATTAAACAGCATGC
GCCAATTGCTGATGGACGGGTGTTTTTAGCAGGTAGTGGCGCTGAAAGTATTGAAATTGCCCTTAAAACTGCCATGCTTG
CAACCCAAAAACCTGGAGTCATTGCATTTACTGGTGGTTATCATGGTTTGAGTTATGGGGCATTGGCGGCAACCAATCGG
GCTGATTTTCGCCAACCATTCTTGCCGCAATTATCGAGCCATATTCAACGTGCACCGTATCCCTATCCATTTCGTTGGCG
TGAGGCTGGTGATTGTTTGGATTGGGCATTAACCACTACTGAGCAGGCGATCAAAACCAGTGCTAGCTCAATTGGGGCAT
TAATTGTCGAGCCAGTTCAAGGCCGTGAAGGCGAAATTGTGCCGCCTGATGGCTGGTTGCGCGGCTTGCGCCAACTGTGT
GATCAATACAATATTTTGTTGATTGCTGATGAAATTTTTACTGGTTGGGGACGCACTGGCAAGTGGTGGGGCGTGAATCA
CGATGGAATCGAGCCAGATTTGATTTGTATGGGCAAGGGCATGACTGGTGGTTTGCAAATTGCCGCCTGTGTTGGCCGTG
CTGAGCATATGGCCTATTGGCAGGTCAATGGCGAACCCTTGCATACTGGCACATTTATGGGTCATCCCTTGGCATGCGCT
GGGGCCGCCGCCGCAATTCGGGTATTAACTGAACATAATACATTAGATCAGGTCAATCAATTAAGCCAAAACTTACTCCG
TGGTTTAGAGGCGATTGCCGAAAACTGCGCCTTAGTGGGTGATGTGCGCGGGCGTGGCTTGATGATCGGGCTAGAGCTGG
TACAAGCTGATGGCATTACGCCCAATCCCGCAGCCGTTATGCAGGTGGTGAGTTTGTGCCAAGCCCAAGGGGTTTTGGTG
CTTGGCGGCGGGATGCATGGCAATGTGCTGATCCTTACACCGCCGTTTATCCTCGATCAAGCTCAAGTTGAATATGGGTT
GAATGTGTTGCAACAAGCCTTGCTCACGGTTGCTGAACAGCAAAAATAG

Upstream 100 bases:

>100_bases
GCGCTCGTTTGTAAAAACTTGAAGCTTGCTACAATACCCATGGTTGCTTTGGCAGCCATGGGTTTTTTGATCCTGAAAAT
AGTTAAGAATGGAATAGAGC

Downstream 100 bases:

>100_bases
CAGCGGAGCAAGGTTGCTCCGCTGCCCAAGCACCTGGAGGAATTGGGCTTTAGCCCCGGCGACCTACATCGCCATCGCGG
TCGATATCACGGTCAAGTTT

Product: class III aminotransferase

Products: succinate semialdehyde; L-glutamate

Alternate protein names: ORF B [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLPKIVSAVPGPRSQALLAQLASSEAPSLTLPGGIVWAEAEGALVTDVDGNRYLDFAAAFGVVGIGHRHPAVLAAIQAQS
QRLIHGMGDVFAHEARIELVQLIKQHAPIADGRVFLAGSGAESIEIALKTAMLATQKPGVIAFTGGYHGLSYGALAATNR
ADFRQPFLPQLSSHIQRAPYPYPFRWREAGDCLDWALTTTEQAIKTSASSIGALIVEPVQGREGEIVPPDGWLRGLRQLC
DQYNILLIADEIFTGWGRTGKWWGVNHDGIEPDLICMGKGMTGGLQIAACVGRAEHMAYWQVNGEPLHTGTFMGHPLACA
GAAAAIRVLTEHNTLDQVNQLSQNLLRGLEAIAENCALVGDVRGRGLMIGLELVQADGITPNPAAVMQVVSLCQAQGVLV
LGGGMHGNVLILTPPFILDQAQVEYGLNVLQQALLTVAEQQK

Sequences:

>Translated_442_residues
MLPKIVSAVPGPRSQALLAQLASSEAPSLTLPGGIVWAEAEGALVTDVDGNRYLDFAAAFGVVGIGHRHPAVLAAIQAQS
QRLIHGMGDVFAHEARIELVQLIKQHAPIADGRVFLAGSGAESIEIALKTAMLATQKPGVIAFTGGYHGLSYGALAATNR
ADFRQPFLPQLSSHIQRAPYPYPFRWREAGDCLDWALTTTEQAIKTSASSIGALIVEPVQGREGEIVPPDGWLRGLRQLC
DQYNILLIADEIFTGWGRTGKWWGVNHDGIEPDLICMGKGMTGGLQIAACVGRAEHMAYWQVNGEPLHTGTFMGHPLACA
GAAAAIRVLTEHNTLDQVNQLSQNLLRGLEAIAENCALVGDVRGRGLMIGLELVQADGITPNPAAVMQVVSLCQAQGVLV
LGGGMHGNVLILTPPFILDQAQVEYGLNVLQQALLTVAEQQK
>Mature_442_residues
MLPKIVSAVPGPRSQALLAQLASSEAPSLTLPGGIVWAEAEGALVTDVDGNRYLDFAAAFGVVGIGHRHPAVLAAIQAQS
QRLIHGMGDVFAHEARIELVQLIKQHAPIADGRVFLAGSGAESIEIALKTAMLATQKPGVIAFTGGYHGLSYGALAATNR
ADFRQPFLPQLSSHIQRAPYPYPFRWREAGDCLDWALTTTEQAIKTSASSIGALIVEPVQGREGEIVPPDGWLRGLRQLC
DQYNILLIADEIFTGWGRTGKWWGVNHDGIEPDLICMGKGMTGGLQIAACVGRAEHMAYWQVNGEPLHTGTFMGHPLACA
GAAAAIRVLTEHNTLDQVNQLSQNLLRGLEAIAENCALVGDVRGRGLMIGLELVQADGITPNPAAVMQVVSLCQAQGVLV
LGGGMHGNVLILTPPFILDQAQVEYGLNVLQQALLTVAEQQK

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=419, Percent_Identity=30.7875894988067, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI4557809, Length=419, Percent_Identity=25.5369928400955, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI37574042, Length=421, Percent_Identity=26.3657957244656, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI226442709, Length=391, Percent_Identity=26.3427109974425, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI24119277, Length=446, Percent_Identity=27.8026905829596, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI226442705, Length=421, Percent_Identity=25.41567695962, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI188536080, Length=478, Percent_Identity=25.1046025104602, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI38679950, Length=478, Percent_Identity=25.1046025104602, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI38679946, Length=478, Percent_Identity=25.1046025104602, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI284507298, Length=227, Percent_Identity=29.9559471365639, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789016, Length=405, Percent_Identity=36.0493827160494, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1787560, Length=399, Percent_Identity=34.8370927318296, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI145693181, Length=414, Percent_Identity=33.8164251207729, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788044, Length=408, Percent_Identity=30.6372549019608, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789759, Length=413, Percent_Identity=28.8135593220339, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1786991, Length=394, Percent_Identity=31.2182741116751, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1786349, Length=344, Percent_Identity=29.0697674418605, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32564660, Length=426, Percent_Identity=28.169014084507, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI25144271, Length=461, Percent_Identity=27.114967462039, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=424, Percent_Identity=25.7075471698113, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=313, Percent_Identity=27.1565495207668, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=475, Percent_Identity=22.9473684210526, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=407, Percent_Identity=29.4840294840295, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6323470, Length=418, Percent_Identity=27.7511961722488, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6321456, Length=414, Percent_Identity=26.5700483091787, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324386, Length=368, Percent_Identity=27.1739130434783, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161085790, Length=439, Percent_Identity=30.2961275626424, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28574759, Length=439, Percent_Identity=29.8405466970387, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21357415, Length=440, Percent_Identity=26.3636363636364, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21356575, Length=413, Percent_Identity=26.3922518159806, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI281366494, Length=407, Percent_Identity=24.5700245700246, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667139, Length=407, Percent_Identity=24.5700245700246, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667143, Length=407, Percent_Identity=24.5700245700246, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19

Molecular weight: Translated: 47003; Mature: 47003

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKIVSAVPGPRSQALLAQLASSEAPSLTLPGGIVWAEAEGALVTDVDGNRYLDFAAAF
CCCCHHHCCCCCCHHHHHHHHHCCCCCCEECCCCEEEEECCCCEEEECCCCCEEHHHHHH
GVVGIGHRHPAVLAAIQAQSQRLIHGMGDVFAHEARIELVQLIKQHAPIADGRVFLAGSG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
AESIEIALKTAMLATQKPGVIAFTGGYHGLSYGALAATNRADFRQPFLPQLSSHIQRAPY
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHEECCCHHCCCCCHHHHHHHHHHCCC
PYPFRWREAGDCLDWALTTTEQAIKTSASSIGALIVEPVQGREGEIVPPDGWLRGLRQLC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCHHHHHHHHHH
DQYNILLIADEIFTGWGRTGKWWGVNHDGIEPDLICMGKGMTGGLQIAACVGRAEHMAYW
HHCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCHHEEEEE
QVNGEPLHTGTFMGHPLACAGAAAAIRVLTEHNTLDQVNQLSQNLLRGLEAIAENCALVG
EECCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEE
DVRGRGLMIGLELVQADGITPNPAAVMQVVSLCQAQGVLVLGGGMHGNVLILTPPFILDQ
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHH
AQVEYGLNVLQQALLTVAEQQK
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPKIVSAVPGPRSQALLAQLASSEAPSLTLPGGIVWAEAEGALVTDVDGNRYLDFAAAF
CCCCHHHCCCCCCHHHHHHHHHCCCCCCEECCCCEEEEECCCCEEEECCCCCEEHHHHHH
GVVGIGHRHPAVLAAIQAQSQRLIHGMGDVFAHEARIELVQLIKQHAPIADGRVFLAGSG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
AESIEIALKTAMLATQKPGVIAFTGGYHGLSYGALAATNRADFRQPFLPQLSSHIQRAPY
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHEECCCHHCCCCCHHHHHHHHHHCCC
PYPFRWREAGDCLDWALTTTEQAIKTSASSIGALIVEPVQGREGEIVPPDGWLRGLRQLC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEECCCHHHHHHHHHH
DQYNILLIADEIFTGWGRTGKWWGVNHDGIEPDLICMGKGMTGGLQIAACVGRAEHMAYW
HHCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCHHEEEEE
QVNGEPLHTGTFMGHPLACAGAAAAIRVLTEHNTLDQVNQLSQNLLRGLEAIAENCALVG
EECCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEE
DVRGRGLMIGLELVQADGITPNPAAVMQVVSLCQAQGVLVLGGGMHGNVLILTPPFILDQ
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHH
AQVEYGLNVLQQALLTVAEQQK
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-aminobutanoate; 2-oxoglutarate

Specific reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA