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The map label for this gene is spsK [H]

Identifier: 159898981

GI number: 159898981

Start: 3144356

End: 3145216

Strand: Direct

Name: spsK [H]

Synonym: Haur_2462

Alternate gene names: 159898981

Gene position: 3144356-3145216 (Clockwise)

Preceding gene: 159898980

Following gene: 159898982

Centisome position: 49.54

GC content: 54.36

Gene sequence:

>861_bases
ATGAGTCGCTTGTTAATTACTGGGGCATCAGGCTATCTTGGCCAACGGGTCGCTTATTTTGCACAGCAGACAACAATCTG
GGATAAAGTGTATAGCCAAACTTGGCGTAACCCGCCAACAGCTGGCGAGCATGTAGCCTGCGATTTGGCCGATCAAGCCC
AACTTGAGGCACTACTTGAGCAAATTCAGCCAACTGCAATTATTCATACGGCGGCGGTCACGCCTGCGATGGGGCCAGCC
ATGACCGACCAAGCCTTGTGGGCCGTGAATGTTCAAGCCAGCGCCACCCTCGCCCGTTGGGCAGCCCAACATCAAGTCCG
ATTGGTGCATGTTTCATCGGATGCAGTGTGGGGTGGACGCGAGGCCGCCTATACCGAAAGCGACGTGCCAGCACCAATCC
ACCCCTATGGAGCCTCGAAGGCAGCGGGCGAGGCAGTTGTCGCAGCGCTTGACCCACAGGCAGCCATCGCTCGCACCTCG
TTGTTGTATGGTCAAAACCCGCTAGACCCCAACACAACCATGGCCTTGGCAATGCTGCGCGGCGAACGTCATGGCGTGCT
TTTTACCGATGAACTGCGCTGCCCAGTCTTTGTTGAAGATGTGGCTCAAGCCTTGATTGAGCTTGCACAAAAACCAAGCC
TTTATGGTATATTTCACCTTGTAGGCCCACAAATTCTCAGTCGATTTGAGCTTGGCCAAGCCCTGCTCGCGTGGAATGGT
ATTGATTCCACAGGCTTGCCTGCTGGTACCACCACTGCCAGTGGCTTACGTCGCCCATCGCGAGTTGTTGTCGATAATCG
CACAACTCAATCTCAACTTAGCACCATTCTTCGAAGCATTGATGATGTCACAAGGAGGTGA

Upstream 100 bases:

>100_bases
AGAGGGGTTGGGGGTGAGGGGGTGAGGAATCAAAAACCCAATACTTGCAGCGTGGGCTACAATAGAACTATTAACGATCT
TTTTAGTGATGGTGTTGTGT

Downstream 100 bases:

>100_bases
CGCATGGAGCGCACACCTGAAACGATTGTTCAATTAGTCGCCGATTTGCAGCACGATGATGAGTTTGTGCGTTCGCAAGC
GGCCTTCACGCTTGGTTTGT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSRLLITGASGYLGQRVAYFAQQTTIWDKVYSQTWRNPPTAGEHVACDLADQAQLEALLEQIQPTAIIHTAAVTPAMGPA
MTDQALWAVNVQASATLARWAAQHQVRLVHVSSDAVWGGREAAYTESDVPAPIHPYGASKAAGEAVVAALDPQAAIARTS
LLYGQNPLDPNTTMALAMLRGERHGVLFTDELRCPVFVEDVAQALIELAQKPSLYGIFHLVGPQILSRFELGQALLAWNG
IDSTGLPAGTTTASGLRRPSRVVVDNRTTQSQLSTILRSIDDVTRR

Sequences:

>Translated_286_residues
MSRLLITGASGYLGQRVAYFAQQTTIWDKVYSQTWRNPPTAGEHVACDLADQAQLEALLEQIQPTAIIHTAAVTPAMGPA
MTDQALWAVNVQASATLARWAAQHQVRLVHVSSDAVWGGREAAYTESDVPAPIHPYGASKAAGEAVVAALDPQAAIARTS
LLYGQNPLDPNTTMALAMLRGERHGVLFTDELRCPVFVEDVAQALIELAQKPSLYGIFHLVGPQILSRFELGQALLAWNG
IDSTGLPAGTTTASGLRRPSRVVVDNRTTQSQLSTILRSIDDVTRR
>Mature_285_residues
SRLLITGASGYLGQRVAYFAQQTTIWDKVYSQTWRNPPTAGEHVACDLADQAQLEALLEQIQPTAIIHTAAVTPAMGPAM
TDQALWAVNVQASATLARWAAQHQVRLVHVSSDAVWGGREAAYTESDVPAPIHPYGASKAAGEAVVAALDPQAAIARTSL
LYGQNPLDPNTTMALAMLRGERHGVLFTDELRCPVFVEDVAQALIELAQKPSLYGIFHLVGPQILSRFELGQALLAWNGI
DSTGLPAGTTTASGLRRPSRVVVDNRTTQSQLSTILRSIDDVTRR

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=272, Percent_Identity=31.6176470588235, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI11034825, Length=272, Percent_Identity=31.6176470588235, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 30786; Mature: 30655

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLITGASGYLGQRVAYFAQQTTIWDKVYSQTWRNPPTAGEHVACDLADQAQLEALLE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHH
QIQPTAIIHTAAVTPAMGPAMTDQALWAVNVQASATLARWAAQHQVRLVHVSSDAVWGGR
HCCCHHEEEHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCCC
EAAYTESDVPAPIHPYGASKAAGEAVVAALDPQAAIARTSLLYGQNPLDPNTTMALAMLR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GERHGVLFTDELRCPVFVEDVAQALIELAQKPSLYGIFHLVGPQILSRFELGQALLAWNG
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
IDSTGLPAGTTTASGLRRPSRVVVDNRTTQSQLSTILRSIDDVTRR
CCCCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLLITGASGYLGQRVAYFAQQTTIWDKVYSQTWRNPPTAGEHVACDLADQAQLEALLE
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHH
QIQPTAIIHTAAVTPAMGPAMTDQALWAVNVQASATLARWAAQHQVRLVHVSSDAVWGGR
HCCCHHEEEHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCCC
EAAYTESDVPAPIHPYGASKAAGEAVVAALDPQAAIARTSLLYGQNPLDPNTTMALAMLR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GERHGVLFTDELRCPVFVEDVAQALIELAQKPSLYGIFHLVGPQILSRFELGQALLAWNG
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
IDSTGLPAGTTTASGLRRPSRVVVDNRTTQSQLSTILRSIDDVTRR
CCCCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]