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The map label for this gene is nirA [H]

Identifier: 159898854

GI number: 159898854

Start: 2978663

End: 2980141

Strand: Direct

Name: nirA [H]

Synonym: Haur_2333

Alternate gene names: 159898854

Gene position: 2978663-2980141 (Clockwise)

Preceding gene: 159898853

Following gene: 159898855

Centisome position: 46.93

GC content: 52.13

Gene sequence:

>1479_bases
ATGGCTGTTCAAATTGAAACCATCAAAAAGGTCAAAAATGGGCTTGATGTCTTGCCTGATCTGTATCGCTATGCTCGCTT
AGGCTTCGATGCGATTCCCGAAGATGAGCTTGAGCGCTTGAAATGGTATGGATTGTTACACCGCAAGCAAACGCCTGGCT
TCTTTATGCAACGCCTGCGCATTCCCAATGGCATTCTGAGCACGCGCCAAATGCGGGCAATCGTCAGCATCTCGCGCGAT
TTTGGCCGCAACACCATGGATTTGACCACCCGCGAAAATATTCAATTGCGCTGGTTGCGCATCGAAGATGTGCCAGAAGT
GTTTCAACGCTTGCAAAATGTTGGCCTAACATCCCAACAAACTGGGCTTGATAACTATCGCAATGTGATGGGCTGCCCCT
TGGCTGGCCTGCACCACGCAGAAATTTTCAACGCTGCCCCCATTGCCCAAAGCGTTTCGTTGGCCTTGCTTGGCCGCGAG
TTCAGCGATTTGCCGCGCAAATTCAATATTACGATCAGCGGTTGCTCGCACGATTGTGCCCATAGCCGAGCCAACGATAT
TGGCATGACTCCTGCCGCCAAAGAAATTAATGGCTATCGTGTGCTCGGTTTCCACGTAGCGCTGGGCGGAGCATTGGGCG
GCACATCGCCGCAACTGGGCCAAGATGCAGGCATCTTCTTAACCACTGAACAAGCCTTGCCTTTCTGTCGCGCGGTCTTG
ACGGTGTTCCGCGACAATGGCTCACGCGAAAAACGCACCGAAGCCCGCTTGAAATGGCTGATTCGCGAATGGGGCATGCC
GCGCTTTATGGCCGAAGTTGAAAAGGTGTTTGGTCAAGCCTTCTTCAGCGCTGGCGAATCGTTGTTGATTGAGCATAGCG
GCGACCACTTGGGCATTCATCAACAACAAGAGGCTGGCTTTGTAACGGTTGGTTTATTAGTGCCAGTTGGTCGAACTAAC
GCCGAGCAAATGGTCGAAATCGCTGATTTGGCCGATGCCTATGGCACTGGCGAACTGCGCTTGACCCCCGATCAAAACAT
TCTGATTCCGAATGTGCACGAAACCTGCCTCGAACGCTTGTTGGCTGAGCCATTGCTACAAGTGCTGCAACCACATGCAC
CTGGGGCGTTGCGCGGTTTGGTCAGTTGTACAGGTCGCGATTATTGCCACTTTGCCTTGAGCGATACCAAAGAATTATCG
TTGCAAGTTGCCACCGAATTGGCCAGCTTAATTCCTGCTGAACGCCGCGTCGATTTGAAAGTTTCGGGCTGCGTACACGC
CTGTGGTCAGCATCACGTTGGCGAAATTGGTTTGCAAGCTCAGCGTTTGCGGCTCGAAGATGGCACAATCGTCGATGCTT
TCGATCTATTTGTTGGTGGCAACCATCAGCAACTGGCAACCCTTAAAGCTCGCAAAATCCCAGTTGATCAATTGGCTGGG
CGGATTGCTGCTGAACTCGCCGTTATGGATGGGGAATAA

Upstream 100 bases:

>100_bases
CACTTTAGCGCCGCCTTCCGCCGTTTATACGATTGCACGCCGCGCCAAATTCGCGAATACGACGATGACTTGATGCTTGA
GGCTGTTATGGAGGAAGCTC

Downstream 100 bases:

>100_bases
GCATCATGAGTAACGTTGTGCGTTCACAATGCCCATATTGTGGCGTTGGCTGTGGAATTGAATTGACCGTCAACGATGGC
AAGGTCGTGCGGGTCAACGG

Product: ferredoxin--nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MAVQIETIKKVKNGLDVLPDLYRYARLGFDAIPEDELERLKWYGLLHRKQTPGFFMQRLRIPNGILSTRQMRAIVSISRD
FGRNTMDLTTRENIQLRWLRIEDVPEVFQRLQNVGLTSQQTGLDNYRNVMGCPLAGLHHAEIFNAAPIAQSVSLALLGRE
FSDLPRKFNITISGCSHDCAHSRANDIGMTPAAKEINGYRVLGFHVALGGALGGTSPQLGQDAGIFLTTEQALPFCRAVL
TVFRDNGSREKRTEARLKWLIREWGMPRFMAEVEKVFGQAFFSAGESLLIEHSGDHLGIHQQQEAGFVTVGLLVPVGRTN
AEQMVEIADLADAYGTGELRLTPDQNILIPNVHETCLERLLAEPLLQVLQPHAPGALRGLVSCTGRDYCHFALSDTKELS
LQVATELASLIPAERRVDLKVSGCVHACGQHHVGEIGLQAQRLRLEDGTIVDAFDLFVGGNHQQLATLKARKIPVDQLAG
RIAAELAVMDGE

Sequences:

>Translated_492_residues
MAVQIETIKKVKNGLDVLPDLYRYARLGFDAIPEDELERLKWYGLLHRKQTPGFFMQRLRIPNGILSTRQMRAIVSISRD
FGRNTMDLTTRENIQLRWLRIEDVPEVFQRLQNVGLTSQQTGLDNYRNVMGCPLAGLHHAEIFNAAPIAQSVSLALLGRE
FSDLPRKFNITISGCSHDCAHSRANDIGMTPAAKEINGYRVLGFHVALGGALGGTSPQLGQDAGIFLTTEQALPFCRAVL
TVFRDNGSREKRTEARLKWLIREWGMPRFMAEVEKVFGQAFFSAGESLLIEHSGDHLGIHQQQEAGFVTVGLLVPVGRTN
AEQMVEIADLADAYGTGELRLTPDQNILIPNVHETCLERLLAEPLLQVLQPHAPGALRGLVSCTGRDYCHFALSDTKELS
LQVATELASLIPAERRVDLKVSGCVHACGQHHVGEIGLQAQRLRLEDGTIVDAFDLFVGGNHQQLATLKARKIPVDQLAG
RIAAELAVMDGE
>Mature_491_residues
AVQIETIKKVKNGLDVLPDLYRYARLGFDAIPEDELERLKWYGLLHRKQTPGFFMQRLRIPNGILSTRQMRAIVSISRDF
GRNTMDLTTRENIQLRWLRIEDVPEVFQRLQNVGLTSQQTGLDNYRNVMGCPLAGLHHAEIFNAAPIAQSVSLALLGREF
SDLPRKFNITISGCSHDCAHSRANDIGMTPAAKEINGYRVLGFHVALGGALGGTSPQLGQDAGIFLTTEQALPFCRAVLT
VFRDNGSREKRTEARLKWLIREWGMPRFMAEVEKVFGQAFFSAGESLLIEHSGDHLGIHQQQEAGFVTVGLLVPVGRTNA
EQMVEIADLADAYGTGELRLTPDQNILIPNVHETCLERLLAEPLLQVLQPHAPGALRGLVSCTGRDYCHFALSDTKELSL
QVATELASLIPAERRVDLKVSGCVHACGQHHVGEIGLQAQRLRLEDGTIVDAFDLFVGGNHQQLATLKARKIPVDQLAGR
IAAELAVMDGE

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322597, Length=504, Percent_Identity=21.6269841269841, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 54372; Mature: 54241

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVQIETIKKVKNGLDVLPDLYRYARLGFDAIPEDELERLKWYGLLHRKQTPGFFMQRLR
CEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
IPNGILSTRQMRAIVSISRDFGRNTMDLTTRENIQLRWLRIEDVPEVFQRLQNVGLTSQQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHCCCCCCC
TGLDNYRNVMGCPLAGLHHAEIFNAAPIAQSVSLALLGREFSDLPRKFNITISGCSHDCA
CCCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHCCCEEEEEEECCCCHHH
HSRANDIGMTPAAKEINGYRVLGFHVALGGALGGTSPQLGQDAGIFLTTEQALPFCRAVL
HCCCCCCCCCCCHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
TVFRDNGSREKRTEARLKWLIREWGMPRFMAEVEKVFGQAFFSAGESLLIEHSGDHLGIH
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
QQQEAGFVTVGLLVPVGRTNAEQMVEIADLADAYGTGELRLTPDQNILIPNVHETCLERL
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHHHH
LAEPLLQVLQPHAPGALRGLVSCTGRDYCHFALSDTKELSLQVATELASLIPAERRVDLK
HHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEE
VSGCVHACGQHHVGEIGLQAQRLRLEDGTIVDAFDLFVGGNHQQLATLKARKIPVDQLAG
EHHHHHHCCCCCCCHHCCCCCEEEECCCCEEEEEHHHCCCCCHHHHHHHHHCCCHHHHHH
RIAAELAVMDGE
HHHHHHEEECCC
>Mature Secondary Structure 
AVQIETIKKVKNGLDVLPDLYRYARLGFDAIPEDELERLKWYGLLHRKQTPGFFMQRLR
EEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
IPNGILSTRQMRAIVSISRDFGRNTMDLTTRENIQLRWLRIEDVPEVFQRLQNVGLTSQQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHCCCCCCC
TGLDNYRNVMGCPLAGLHHAEIFNAAPIAQSVSLALLGREFSDLPRKFNITISGCSHDCA
CCCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHCCCEEEEEEECCCCHHH
HSRANDIGMTPAAKEINGYRVLGFHVALGGALGGTSPQLGQDAGIFLTTEQALPFCRAVL
HCCCCCCCCCCCHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
TVFRDNGSREKRTEARLKWLIREWGMPRFMAEVEKVFGQAFFSAGESLLIEHSGDHLGIH
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
QQQEAGFVTVGLLVPVGRTNAEQMVEIADLADAYGTGELRLTPDQNILIPNVHETCLERL
CCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEECCCHHHHHHHHH
LAEPLLQVLQPHAPGALRGLVSCTGRDYCHFALSDTKELSLQVATELASLIPAERRVDLK
HHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEE
VSGCVHACGQHHVGEIGLQAQRLRLEDGTIVDAFDLFVGGNHQQLATLKARKIPVDQLAG
EHHHHHHCCCCCCCHHCCCCCEEEECCCCEEEEEHHHCCCCCHHHHHHHHHCCCHHHHHH
RIAAELAVMDGE
HHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]