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The map label for this gene is nrgA [H]

Identifier: 159898476

GI number: 159898476

Start: 2367205

End: 2368440

Strand: Reverse

Name: nrgA [H]

Synonym: Haur_1952

Alternate gene names: 159898476

Gene position: 2368440-2367205 (Counterclockwise)

Preceding gene: 159898478

Following gene: 159898475

Centisome position: 37.32

GC content: 53.32

Gene sequence:

>1236_bases
ATGGATATGATTAACGGGGCAGATACCGTTTGGGTGCTTCTCTCAGCCGCCTTGGTTATGTTGATGACTCCGGCCTTAGG
CCTCTTTTATGGTGGGATGGTTCGCCAAAAAAATGTGCTTTCAACCTTGATGCACAGCTTTTTTATGTTGGCCCTGATTA
GTGTGCAATGGGTGCTGTTTGGCTATAGTTTAGCCTTTGGCCCCGATGTTGGCGGGGTAATCGGCAGCCTCGAATGGGCT
GGCTTGCGCAATGTTGGGATTGAACCAAACCCAACCTATGCGGCAACGATTCCGCATCAAGCTTTCATGATCTATCAGAT
GATGTTTGCGGTCATCACGCCAGCCTTGATTTCAGGTGCATTTGCTGAACGCAAGCGCTTTAAATCCTTCGTAATTTTCT
CGCTGGCTTGGTCAACCCTGATTTATGCCCCAGTTGCGCATTGGGTTTGGGCTGATGGCGGTTGGTTACGCTCGTTGGGA
GCCTTAGACTTTGCTGGTGGCACGGTCGTACACATCACCTCAGGGGTTTCGGCGCTGGTTTGTGCCTTGGTGCTTGGCCG
TCGCTCAGGCTATGGCAAAGAAGCAATGGAGCCACACGATACAACTATGACCGTGATTGGCACTGCCTTATTGTGGTTCG
GCTGGTTTGGCTTCAATGCAGGTAGCTCGCTTGGCGCTGGTGGCTTGGCCGTTTCAGCCTTCGTCAACACCAACTCAGCC
GCTGCCATGGCTATGCTTGCTTGGATGACCGCTGGCTGGATTCAACACAAACGCCCAAGCGTACTCGGCGCTTCGGCTGG
CGCAGTCGCTGGTTTGGTGGCGATCACGCCTGCTGCGGGCTTCGTTACCCCAATGGCCTCATTGATTATCGGCTTCTTAG
CTGGTGGCATCTGCTACTTCGTGGTCGAAAACTTGGTGCGTGGTCGGGTTGACGATGCGCTTGATGTCTTCGGCGTACAC
GGTGTTGGCGGGGTGTTGGGGGCAATTTTGGTTGGCGTATTCGCAACCAAGAGCATCAATCCTGCTGGTGCTGATGGCGT
ACTTTCGGGCAATTGGAGCCAACTTGGCGTACAAGCGCTCACGGTTGGGATTGTGGCAATCTATGCCGCTGGCATGACCT
GGGGCATTCTTAAAGTCGTCGATCTCTTGTTTGGCATTCGGGTTAGCGAAGCCGAAGAAGAACAAGGCTTGGATAGCAGT
CAACATGGCGAAGTGGCCTATCGGATGGGAATTTAG

Upstream 100 bases:

>100_bases
ACCAAACGAGCACAACCAAACGATCCCCAGCGCTGCGGATCACGAGCGTTCAAGCAGTTCAGCGTCCGACATTCAGCACA
AGTTAGGGAGACCACGCAGC

Downstream 100 bases:

>100_bases
TTCATACTGTTGATAAGGAGCTAGCTCATGGAATTCATCATTGCATTGGTTCTGTTCGTTGCGTTGGTTGCCGTTTGGTT
GCGCATGCCTCAAGGCGCAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDMINGADTVWVLLSAALVMLMTPALGLFYGGMVRQKNVLSTLMHSFFMLALISVQWVLFGYSLAFGPDVGGVIGSLEWA
GLRNVGIEPNPTYAATIPHQAFMIYQMMFAVITPALISGAFAERKRFKSFVIFSLAWSTLIYAPVAHWVWADGGWLRSLG
ALDFAGGTVVHITSGVSALVCALVLGRRSGYGKEAMEPHDTTMTVIGTALLWFGWFGFNAGSSLGAGGLAVSAFVNTNSA
AAMAMLAWMTAGWIQHKRPSVLGASAGAVAGLVAITPAAGFVTPMASLIIGFLAGGICYFVVENLVRGRVDDALDVFGVH
GVGGVLGAILVGVFATKSINPAGADGVLSGNWSQLGVQALTVGIVAIYAAGMTWGILKVVDLLFGIRVSEAEEEQGLDSS
QHGEVAYRMGI

Sequences:

>Translated_411_residues
MDMINGADTVWVLLSAALVMLMTPALGLFYGGMVRQKNVLSTLMHSFFMLALISVQWVLFGYSLAFGPDVGGVIGSLEWA
GLRNVGIEPNPTYAATIPHQAFMIYQMMFAVITPALISGAFAERKRFKSFVIFSLAWSTLIYAPVAHWVWADGGWLRSLG
ALDFAGGTVVHITSGVSALVCALVLGRRSGYGKEAMEPHDTTMTVIGTALLWFGWFGFNAGSSLGAGGLAVSAFVNTNSA
AAMAMLAWMTAGWIQHKRPSVLGASAGAVAGLVAITPAAGFVTPMASLIIGFLAGGICYFVVENLVRGRVDDALDVFGVH
GVGGVLGAILVGVFATKSINPAGADGVLSGNWSQLGVQALTVGIVAIYAAGMTWGILKVVDLLFGIRVSEAEEEQGLDSS
QHGEVAYRMGI
>Mature_411_residues
MDMINGADTVWVLLSAALVMLMTPALGLFYGGMVRQKNVLSTLMHSFFMLALISVQWVLFGYSLAFGPDVGGVIGSLEWA
GLRNVGIEPNPTYAATIPHQAFMIYQMMFAVITPALISGAFAERKRFKSFVIFSLAWSTLIYAPVAHWVWADGGWLRSLG
ALDFAGGTVVHITSGVSALVCALVLGRRSGYGKEAMEPHDTTMTVIGTALLWFGWFGFNAGSSLGAGGLAVSAFVNTNSA
AAMAMLAWMTAGWIQHKRPSVLGASAGAVAGLVAITPAAGFVTPMASLIIGFLAGGICYFVVENLVRGRVDDALDVFGVH
GVGGVLGAILVGVFATKSINPAGADGVLSGNWSQLGVQALTVGIVAIYAAGMTWGILKVVDLLFGIRVSEAEEEQGLDSS
QHGEVAYRMGI

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=46.1916461916462, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17550180, Length=423, Percent_Identity=26.0047281323877, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=27.8801843317972, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=27.803738317757, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=27.803738317757, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=419, Percent_Identity=38.4248210023866, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=37.1428571428571, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=35.6459330143541, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI161078314, Length=412, Percent_Identity=30.3398058252427, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43079; Mature: 43079

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMINGADTVWVLLSAALVMLMTPALGLFYGGMVRQKNVLSTLMHSFFMLALISVQWVLF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYSLAFGPDVGGVIGSLEWAGLRNVGIEPNPTYAATIPHQAFMIYQMMFAVITPALISGA
HHHHHCCCCCCHHHHCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
FAERKRFKSFVIFSLAWSTLIYAPVAHWVWADGGWLRSLGALDFAGGTVVHITSGVSALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCHHHHH
CALVLGRRSGYGKEAMEPHDTTMTVIGTALLWFGWFGFNAGSSLGAGGLAVSAFVNTNSA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHH
AAMAMLAWMTAGWIQHKRPSVLGASAGAVAGLVAITPAAGFVTPMASLIIGFLAGGICYF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VVENLVRGRVDDALDVFGVHGVGGVLGAILVGVFATKSINPAGADGVLSGNWSQLGVQAL
HHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TVGIVAIYAAGMTWGILKVVDLLFGIRVSEAEEEQGLDSSQHGEVAYRMGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MDMINGADTVWVLLSAALVMLMTPALGLFYGGMVRQKNVLSTLMHSFFMLALISVQWVLF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYSLAFGPDVGGVIGSLEWAGLRNVGIEPNPTYAATIPHQAFMIYQMMFAVITPALISGA
HHHHHCCCCCCHHHHCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
FAERKRFKSFVIFSLAWSTLIYAPVAHWVWADGGWLRSLGALDFAGGTVVHITSGVSALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCHHHHH
CALVLGRRSGYGKEAMEPHDTTMTVIGTALLWFGWFGFNAGSSLGAGGLAVSAFVNTNSA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHH
AAMAMLAWMTAGWIQHKRPSVLGASAGAVAGLVAITPAAGFVTPMASLIIGFLAGGICYF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VVENLVRGRVDDALDVFGVHGVGGVLGAILVGVFATKSINPAGADGVLSGNWSQLGVQAL
HHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TVGIVAIYAAGMTWGILKVVDLLFGIRVSEAEEEQGLDSSQHGEVAYRMGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]