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The map label for this gene is tpiA

Identifier: 159898447

GI number: 159898447

Start: 2320765

End: 2321517

Strand: Reverse

Name: tpiA

Synonym: Haur_1923

Alternate gene names: 159898447

Gene position: 2321517-2320765 (Counterclockwise)

Preceding gene: 159898448

Following gene: 159898434

Centisome position: 36.58

GC content: 54.71

Gene sequence:

>753_bases
ATGCGCCGACCATTGTTGGCTGGTAATTGGAAAATGCACTACGGGGTCAGTGAGGGTGTGGCCTTAGTCGAAGCACTCAG
CGCCGATTTGACCGATCTAACCGATCGTGATGTGTTGGTTTGCCCACCATTCACCTTGTTGGGCAGTTTAGCACCATTAC
TCGATGGCACTGCCGTGGCCTTGGGTGCGCAAAATATGCACTACGAAGCCAAGGGAGCTTATACTGGCGAAATTGCGCCC
CAAATGCTCAAGGAATTGGGCTGTAGCTATGTGATTTTAGGCCATAGCGAACGCCGCCAGTATTTTGGCGAAACTGATGC
GTTGATCAATCGCAAAGCCCATGCCGCCTTAGCCAACGGCCTCAAGCCAATCGTGTGTGTTGGCGAAGTCAAAGCCGAAC
GCGATAGTGGCCAAGCAGAGAGCGTCGTTGTTGGGCAGTTACGCGGCAGTTTGGCTGGATTAAGTGCCGAGCAATTGCGC
GGGGTCGTGATTGCCTACGAACCAGTTTGGGCGATTGGCACTGGCGATACAGCCACTCCAGCCGATGCCCAAGCCATGCA
CGCCCGGATTCGCGCCGAATTGGCCGCGCTCAGCGATCAAGCAACCGCTGATGCGGTGATTATCCAGTATGGTGGCTCGG
TCAAGCCTGATAACGTCGATGAATTGATGGCTCAGCCGGATATCGACGGAGCCTTGGTTGGCGGAGCCTCGCTCAAAGCT
GCCGATTTCATTCGGATTGTGCGGTTTAAATAG

Upstream 100 bases:

>100_bases
TGCCATGGCCTCAGTTGGCGGGCTGTTGTTTGTTGTACAATACCTGCCGATTGCCACAAACTTATTTGTTTTTGGCTTCT
AAAGCTTTTTTGGAGGATGT

Downstream 100 bases:

>100_bases
TAGGGGTCAGGGGCTAGGGATCAGGGGTTAGTTCCTGATCCCTTTTTCGATCCACGAAGAACACGAAGCACACGAAGGCT
TGATTTTAGCCACGAATTCC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRRPLLAGNWKMHYGVSEGVALVEALSADLTDLTDRDVLVCPPFTLLGSLAPLLDGTAVALGAQNMHYEAKGAYTGEIAP
QMLKELGCSYVILGHSERRQYFGETDALINRKAHAALANGLKPIVCVGEVKAERDSGQAESVVVGQLRGSLAGLSAEQLR
GVVIAYEPVWAIGTGDTATPADAQAMHARIRAELAALSDQATADAVIIQYGGSVKPDNVDELMAQPDIDGALVGGASLKA
ADFIRIVRFK

Sequences:

>Translated_250_residues
MRRPLLAGNWKMHYGVSEGVALVEALSADLTDLTDRDVLVCPPFTLLGSLAPLLDGTAVALGAQNMHYEAKGAYTGEIAP
QMLKELGCSYVILGHSERRQYFGETDALINRKAHAALANGLKPIVCVGEVKAERDSGQAESVVVGQLRGSLAGLSAEQLR
GVVIAYEPVWAIGTGDTATPADAQAMHARIRAELAALSDQATADAVIIQYGGSVKPDNVDELMAQPDIDGALVGGASLKA
ADFIRIVRFK
>Mature_250_residues
MRRPLLAGNWKMHYGVSEGVALVEALSADLTDLTDRDVLVCPPFTLLGSLAPLLDGTAVALGAQNMHYEAKGAYTGEIAP
QMLKELGCSYVILGHSERRQYFGETDALINRKAHAALANGLKPIVCVGEVKAERDSGQAESVVVGQLRGSLAGLSAEQLR
GVVIAYEPVWAIGTGDTATPADAQAMHARIRAELAALSDQATADAVIIQYGGSVKPDNVDELMAQPDIDGALVGGASLKA
ADFIRIVRFK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=43.3734939759036, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=43.3734939759036, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=47.1774193548387, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=47.6, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=41.7670682730924, Blast_Score=172, Evalue=4e-44,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=45.748987854251, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=45.748987854251, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=45.748987854251, Blast_Score=198, Evalue=2e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_HERA2 (A9AUN9)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001544694.1
- ProteinModelPortal:   A9AUN9
- SMR:   A9AUN9
- GeneID:   5733812
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1923
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   HAUR316274:HAUR_1923-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26351; Mature: 26351

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPLLAGNWKMHYGVSEGVALVEALSADLTDLTDRDVLVCPPFTLLGSLAPLLDGTAVA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEE
LGAQNMHYEAKGAYTGEIAPQMLKELGCSYVILGHSERRQYFGETDALINRKAHAALANG
ECCCCCCEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHCC
LKPIVCVGEVKAERDSGQAESVVVGQLRGSLAGLSAEQLRGVVIAYEPVWAIGTGDTATP
CCEEEEEECEECCCCCCCCHHEEHHHHHHHHCCCCHHHHCCEEEEECCEEEEECCCCCCC
ADAQAMHARIRAELAALSDQATADAVIIQYGGSVKPDNVDELMAQPDIDGALVGGASLKA
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCH
ADFIRIVRFK
HHEEEEEECC
>Mature Secondary Structure
MRRPLLAGNWKMHYGVSEGVALVEALSADLTDLTDRDVLVCPPFTLLGSLAPLLDGTAVA
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEE
LGAQNMHYEAKGAYTGEIAPQMLKELGCSYVILGHSERRQYFGETDALINRKAHAALANG
ECCCCCCEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHCC
LKPIVCVGEVKAERDSGQAESVVVGQLRGSLAGLSAEQLRGVVIAYEPVWAIGTGDTATP
CCEEEEEECEECCCCCCCCHHEEHHHHHHHHCCCCHHHHCCEEEEECCEEEEECCCCCCC
ADAQAMHARIRAELAALSDQATADAVIIQYGGSVKPDNVDELMAQPDIDGALVGGASLKA
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCH
ADFIRIVRFK
HHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA