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The map label for this gene is rsbRA [H]

Identifier: 159898427

GI number: 159898427

Start: 2295897

End: 2297966

Strand: Reverse

Name: rsbRA [H]

Synonym: Haur_1903

Alternate gene names: 159898427

Gene position: 2297966-2295897 (Counterclockwise)

Preceding gene: 159898434

Following gene: 159898418

Centisome position: 36.21

GC content: 49.52

Gene sequence:

>2070_bases
ATGGCTAGCAGGCAGGCAATTGGTTTCATTGCACCATCACTCGCCGGCGAATTTATGACAGGCGTTATGGAAGGCATTTT
GCAAAGCTTACGCAAGCATCAATACGATTTGATTGCGGTGCAAGGTGCTCCCCAACATCTGATCAACAACCAACTGGCCA
AACAGCATATCAAAGCTTGGATTGTGATTATCGATCCGACTGGGTTAAATGAATTAGCGACCAACATGCCAGCCGTCGTC
ATTAGTGCCCCCAATGCTGGCGAGCGCTTTCCGCTGGTCATGCCCGATAACAACCAAAGTGCCCGGGCGATGGTCGAACA
CCTGATCGAGCATGGTCATAGCAAAATCGCCTTCGTTGGCAATTTTAATAATAGCGATATGCGCGATCGCTACACGGTAT
ATTGCGATGTGCTCAACGAACATAGCATCACGCCTAACCCGGCTTGGGTGGTCGATGTTGGCGATTATACCGAGGGTCGC
ACCGAACATGCCTTCTACCCGCTGATTGCCAATGGGTTAAATTTTAGTGCCGTGGTCGCCGCCAACGATCAACAAGCAGT
TGGGGTAATGATGGCCTTGGAAACTAAGGGTTATCGAGTGCCACACGATATGGCAGTTGTGGGCTTTGATGATGCCACTA
TTGCCCAACATGCCAACCCACCATTAACCACCGTGCGTCAACAACCCCAATTGCTTGGCAGCATCGCCGCCGATACTGTT
ATTGAGCTGATCGCAGGTAAACCGGTTTCAAACGCCCCAATCTATGTGCCAACCATCCCCATTTGGCGGCAATCGTGTGG
CTGTAAAGATGTTTATACCGGAATTAAGACCAGTACAGTTGCGCCCAAACAGCAACATTGGGCCGATCAATTAGCCCAAC
GTATGCAGCAAGTGATCTATTATCCTGTGCCAATCGATCCCAATCTCAGTCTCAACGAGAGCAACCAAGGGATTCGCAGC
CTGATTCAGGCACTTCAGGCTAAGGTTGAGGCCCAAAACGTTGAGGTCGATTATTTGGCGGCATGGTATGCAATTATCAC
GGTTAGCTCCAAATCGGATACGCTTTCGGTGCTGTATCAGGTGTTTGCTACTGCCGCTAATCAACAATTAGCTCAAATCG
ATCTGCCTGCCGATCAGATTAATGGCTTCTTGGCCACCGCCCATACAAGCTTGCTGCATACCGTGCTTGAGCTACAACGG
AACGAAATTACTGGGCTTGATCGGTTGCTCGCTGCCAATCATGAGCTGAGCATGCTCCTCGTTTCAAATCAAAAACAACA
AACTGGCAAATTAGCGTGGATGCAGCGCTTGCCGGTTTCGATCGCTTGTCTAGCGCTATGGGATGAGCAGAAACCAGGTT
TTTTACGGCTTGCCAGCATTTATAGCAAAGATGGCTCGTCAACCATCAAAGCAGGCAGCCATTGTCACGGCAGCGAGTTT
CCGCCAGCCGAATTTCTCGAGCAAGCTCGCCAATTGAATAGCAGCGGCTATGTTGCCTGTGTGCCGATCGTCACTGCCAG
CCACGATTGGGGTATTTTGGCTTTTGGCCATGCTGATGTCCAAAAAACAATCGCTGATATTAATAGTTTGTATATTTGGG
CTTCGATTTTGGGCAGTTCGCTCGAACATGAAGCGCTTGAGTCAACGCTTGACGCTGAACGCGAGGTTTTGCGCGGAGCA
GTTGATCGTGAACATGCGTTATCCAAAACAATTTTAGAGCTTGGTTGCCCATTAATTCCTCTATTACCAGGTGTATTGTT
AATTCCTTTAATTGGCAGCATCAACACCGAGCGTGCTCAGCAAATCACTGAAACCGTGCTGAATGGTATCAACGAACAAC
AGGCTAGTCGGGTGCTGCTCGATATTACCGGGGTTTCGATGGTCGATACTCACGTTGCTAGCTCACTCATGCAAATTGCT
CGGGCGGCGATGTTGCTGGGAGCAAAGGTAGCCTTGGTTGGGGTGCGCCCCGATATCGCCCAAAGCATCGTCAGTTTGGG
GATTAACCTCAATACCCTGGTTACCTACCCCAATTTGCGCACTGCGATTCAACAAATGGCAATGGTTTAA

Upstream 100 bases:

>100_bases
TTTGCCAGAGAATCCCCTTGTCAGGGCCAATAATTAGTGCCATACTCAGCTAGATAGCTAGTTTCGGCTAGGTTGCTGTT
GTTTTTCGAGGAGTTTGCAT

Downstream 100 bases:

>100_bases
ATGATCAAGCCGTAGCATGTTGATTGTGCTACGGCTTGATTAAATTATTGCTGTAAACCGAGGGCTTCGACCGTCAGCGC
ATCAATTTCACGCTGACCAC

Product: anti-sigma-factor antagonist

Products: NA

Alternate protein names: Stressosome protein rsbRA [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MASRQAIGFIAPSLAGEFMTGVMEGILQSLRKHQYDLIAVQGAPQHLINNQLAKQHIKAWIVIIDPTGLNELATNMPAVV
ISAPNAGERFPLVMPDNNQSARAMVEHLIEHGHSKIAFVGNFNNSDMRDRYTVYCDVLNEHSITPNPAWVVDVGDYTEGR
TEHAFYPLIANGLNFSAVVAANDQQAVGVMMALETKGYRVPHDMAVVGFDDATIAQHANPPLTTVRQQPQLLGSIAADTV
IELIAGKPVSNAPIYVPTIPIWRQSCGCKDVYTGIKTSTVAPKQQHWADQLAQRMQQVIYYPVPIDPNLSLNESNQGIRS
LIQALQAKVEAQNVEVDYLAAWYAIITVSSKSDTLSVLYQVFATAANQQLAQIDLPADQINGFLATAHTSLLHTVLELQR
NEITGLDRLLAANHELSMLLVSNQKQQTGKLAWMQRLPVSIACLALWDEQKPGFLRLASIYSKDGSSTIKAGSHCHGSEF
PPAEFLEQARQLNSSGYVACVPIVTASHDWGILAFGHADVQKTIADINSLYIWASILGSSLEHEALESTLDAEREVLRGA
VDREHALSKTILELGCPLIPLLPGVLLIPLIGSINTERAQQITETVLNGINEQQASRVLLDITGVSMVDTHVASSLMQIA
RAAMLLGAKVALVGVRPDIAQSIVSLGINLNTLVTYPNLRTAIQQMAMV

Sequences:

>Translated_689_residues
MASRQAIGFIAPSLAGEFMTGVMEGILQSLRKHQYDLIAVQGAPQHLINNQLAKQHIKAWIVIIDPTGLNELATNMPAVV
ISAPNAGERFPLVMPDNNQSARAMVEHLIEHGHSKIAFVGNFNNSDMRDRYTVYCDVLNEHSITPNPAWVVDVGDYTEGR
TEHAFYPLIANGLNFSAVVAANDQQAVGVMMALETKGYRVPHDMAVVGFDDATIAQHANPPLTTVRQQPQLLGSIAADTV
IELIAGKPVSNAPIYVPTIPIWRQSCGCKDVYTGIKTSTVAPKQQHWADQLAQRMQQVIYYPVPIDPNLSLNESNQGIRS
LIQALQAKVEAQNVEVDYLAAWYAIITVSSKSDTLSVLYQVFATAANQQLAQIDLPADQINGFLATAHTSLLHTVLELQR
NEITGLDRLLAANHELSMLLVSNQKQQTGKLAWMQRLPVSIACLALWDEQKPGFLRLASIYSKDGSSTIKAGSHCHGSEF
PPAEFLEQARQLNSSGYVACVPIVTASHDWGILAFGHADVQKTIADINSLYIWASILGSSLEHEALESTLDAEREVLRGA
VDREHALSKTILELGCPLIPLLPGVLLIPLIGSINTERAQQITETVLNGINEQQASRVLLDITGVSMVDTHVASSLMQIA
RAAMLLGAKVALVGVRPDIAQSIVSLGINLNTLVTYPNLRTAIQQMAMV
>Mature_688_residues
ASRQAIGFIAPSLAGEFMTGVMEGILQSLRKHQYDLIAVQGAPQHLINNQLAKQHIKAWIVIIDPTGLNELATNMPAVVI
SAPNAGERFPLVMPDNNQSARAMVEHLIEHGHSKIAFVGNFNNSDMRDRYTVYCDVLNEHSITPNPAWVVDVGDYTEGRT
EHAFYPLIANGLNFSAVVAANDQQAVGVMMALETKGYRVPHDMAVVGFDDATIAQHANPPLTTVRQQPQLLGSIAADTVI
ELIAGKPVSNAPIYVPTIPIWRQSCGCKDVYTGIKTSTVAPKQQHWADQLAQRMQQVIYYPVPIDPNLSLNESNQGIRSL
IQALQAKVEAQNVEVDYLAAWYAIITVSSKSDTLSVLYQVFATAANQQLAQIDLPADQINGFLATAHTSLLHTVLELQRN
EITGLDRLLAANHELSMLLVSNQKQQTGKLAWMQRLPVSIACLALWDEQKPGFLRLASIYSKDGSSTIKAGSHCHGSEFP
PAEFLEQARQLNSSGYVACVPIVTASHDWGILAFGHADVQKTIADINSLYIWASILGSSLEHEALESTLDAEREVLRGAV
DREHALSKTILELGCPLIPLLPGVLLIPLIGSINTERAQQITETVLNGINEQQASRVLLDITGVSMVDTHVASSLMQIAR
AAMLLGAKVALVGVRPDIAQSIVSLGINLNTLVTYPNLRTAIQQMAMV

Specific function: Negative regulator of sigma-B activity. Non- phosphorylated rsbS binds to rsbT, preventing its association with rsbU. Requires any one of rsbRA, rsbRB, rsbRC or rsbRD to sequester rsbT. When rsbS and the rsbR paralog(s) are phosphorylated, they release rs

COG id: COG1366

COG function: function code T; Anti-anti-sigma regulatory factor (antagonist of anti-sigma factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=181, Percent_Identity=33.7016574585635, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789068, Length=132, Percent_Identity=35.6060606060606, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786540, Length=237, Percent_Identity=27.8481012658228, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788474, Length=287, Percent_Identity=27.5261324041812, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790194, Length=249, Percent_Identity=26.1044176706827, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787948, Length=254, Percent_Identity=23.2283464566929, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1790369, Length=257, Percent_Identity=23.7354085603113, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994940, Length=266, Percent_Identity=26.6917293233083, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1787906, Length=274, Percent_Identity=24.4525547445255, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014792
- InterPro:   IPR002645 [H]

Pfam domain/function: PF08678 Rsbr_N; PF01740 STAS [H]

EC number: NA

Molecular weight: Translated: 74932; Mature: 74801

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRQAIGFIAPSLAGEFMTGVMEGILQSLRKHQYDLIAVQGAPQHLINNQLAKQHIKAW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCEEE
IVIIDPTGLNELATNMPAVVISAPNAGERFPLVMPDNNQSARAMVEHLIEHGHSKIAFVG
EEEECCCCCHHHHCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEE
NFNNSDMRDRYTVYCDVLNEHSITPNPAWVVDVGDYTEGRTEHAFYPLIANGLNFSAVVA
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEE
ANDQQAVGVMMALETKGYRVPHDMAVVGFDDATIAQHANPPLTTVRQQPQLLGSIAADTV
CCCCCHHEEEEEEECCCCCCCCCEEEEECCCHHHHHCCCCCHHHHHHCHHHHHHHHHHHH
IELIAGKPVSNAPIYVPTIPIWRQSCGCKDVYTGIKTSTVAPKQQHWADQLAQRMQQVIY
HHHHCCCCCCCCCEEECCCHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEE
YPVPIDPNLSLNESNQGIRSLIQALQAKVEAQNVEVDYLAAWYAIITVSSKSDTLSVLYQ
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEECCCCHHHHHHHH
VFATAANQQLAQIDLPADQINGFLATAHTSLLHTVLELQRNEITGLDRLLAANHELSMLL
HHHHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VSNQKQQTGKLAWMQRLPVSIACLALWDEQKPGFLRLASIYSKDGSSTIKAGSHCHGSEF
ECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCC
PPAEFLEQARQLNSSGYVACVPIVTASHDWGILAFGHADVQKTIADINSLYIWASILGSS
CHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
LEHEALESTLDAEREVLRGAVDREHALSKTILELGCPLIPLLPGVLLIPLIGSINTERAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
QITETVLNGINEQQASRVLLDITGVSMVDTHVASSLMQIARAAMLLGAKVALVGVRPDIA
HHHHHHHCCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHH
QSIVSLGINLNTLVTYPNLRTAIQQMAMV
HHHHHHCCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
ASRQAIGFIAPSLAGEFMTGVMEGILQSLRKHQYDLIAVQGAPQHLINNQLAKQHIKAW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCEEE
IVIIDPTGLNELATNMPAVVISAPNAGERFPLVMPDNNQSARAMVEHLIEHGHSKIAFVG
EEEECCCCCHHHHCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEE
NFNNSDMRDRYTVYCDVLNEHSITPNPAWVVDVGDYTEGRTEHAFYPLIANGLNFSAVVA
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEE
ANDQQAVGVMMALETKGYRVPHDMAVVGFDDATIAQHANPPLTTVRQQPQLLGSIAADTV
CCCCCHHEEEEEEECCCCCCCCCEEEEECCCHHHHHCCCCCHHHHHHCHHHHHHHHHHHH
IELIAGKPVSNAPIYVPTIPIWRQSCGCKDVYTGIKTSTVAPKQQHWADQLAQRMQQVIY
HHHHCCCCCCCCCEEECCCHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEE
YPVPIDPNLSLNESNQGIRSLIQALQAKVEAQNVEVDYLAAWYAIITVSSKSDTLSVLYQ
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEEEECCCCHHHHHHHH
VFATAANQQLAQIDLPADQINGFLATAHTSLLHTVLELQRNEITGLDRLLAANHELSMLL
HHHHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VSNQKQQTGKLAWMQRLPVSIACLALWDEQKPGFLRLASIYSKDGSSTIKAGSHCHGSEF
ECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCC
PPAEFLEQARQLNSSGYVACVPIVTASHDWGILAFGHADVQKTIADINSLYIWASILGSS
CHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
LEHEALESTLDAEREVLRGAVDREHALSKTILELGCPLIPLLPGVLLIPLIGSINTERAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
QITETVLNGINEQQASRVLLDITGVSMVDTHVASSLMQIARAAMLLGAKVALVGVRPDIA
HHHHHHHCCCCHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHH
QSIVSLGINLNTLVTYPNLRTAIQQMAMV
HHHHHHCCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 9384377; 11157946; 10329124 [H]