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The map label for this gene is hsdM [H]

Identifier: 159897811

GI number: 159897811

Start: 1492225

End: 1493670

Strand: Reverse

Name: hsdM [H]

Synonym: Haur_1282

Alternate gene names: 159897811

Gene position: 1493670-1492225 (Counterclockwise)

Preceding gene: 159897812

Following gene: 159897810

Centisome position: 23.54

GC content: 48.55

Gene sequence:

>1446_bases
ATGAATCTCACTACAACCATCAAGAGCATTCAGGATATTATGCGCAAGGATGCTGGGGTCGATGGCGATGCCCAACGGAT
TGGTCAATTGGTTTGGTTGCTTTTCCTCAAAATCTTCGATGATCGCGAGCAGGAATTAGCACTGATTGACCCACATTATC
GTTCGCCCATGCCCGAGGGCTTGGCTTGGCATCAGTGGGCCATCAATCGCGAAGGCATCACAGGCGAGGAATTGCTCAAT
TTTGTCAATAACCAGCTGTTTCCCCAATTGAAAAATCTTGCAGCTACCAATCAGGCCAAGGCCATGATTCAGTCGGTGTT
CGAGGATGCCTATAATTACATGAAAAATGGCACGCTCTTACGCCAAGTGATCAATAAAATCAATGAAATTGATTTCAATC
GCTCTGCCGATCGTCATCTCTTTAACGATGTCTATGAAAAGTTGCTCAGCGATTTACAAGCCGCTGGCAATGCTGGCGAA
TATTATACGCCGCGCACGGTTACGCAGTTTATGATCGAAATGCTCAAGCCCCGTTTAGGCGAGAGCTTGCTCGACCCAGC
CGCTGGTACTGGCGGATTTTTAGTCTCAGCCGTGGAGTATATTCGGCGTAACGATGTCCACACGCCTAGCGATTTGGAAA
CCTTGCAAGCCAATATTCGTGGGATTGAGAAAAAACCGCTGCCGCATTTGTTGGGCATCACCAACTTAATTTTGCATGGA
ATTAATCTGCCCAATTTGCAACACGCCAACACCTTGGCCCGCTCCTACAGCAGCTATGGGGTTGATGATCAAGTCGATAT
TATCGCGACGAACCCACCGTTTGGCGGCCAAGAAGAAGATGGGATTGAAAACAACTTCCCTGAACTCTTCCGCACCCGCG
AAACTGCTGATTTGTTCTTGGTCTTGATTATGCGCTTGCTCAAGCCCAACGGTCGGGCGGCGCTTATTTTGCCCGATGGC
ACGCTTTTTGGCGAAGGCATCAAAACTCGGATCAAGGAAGAATTGCTGAAAAATTGTAATTTGCATACGATTGTGCGCTT
GCCCAATGGGGTTTTTAACCCGTACACCGGAATTAAAACCAATTTATTGTTTTTTGAGAAGGGCCAGCCTACCCAAGAGA
TTTGGTATTACGAGCACCCCTACCCCGCTGGCTATAAATCGTATTCCAAAACTAAGCCGATTCGGCTTGAGGAGTTTGAA
CCAGAACGGGCTTGGTGGATCAATCGTCAAACCAATCAGTTTGCTTGGAAGGTCGATATCGCTGACATTCGGGCCAATAA
TTTCAACTTGGATTGGAAAAACCCCAATCGTCAAGCCGATGCCCAAACTGACCCCTATCAATTATTGGCTGAATACCAAG
CCGCTACTGCCAGCGCCGCCCAAACCCGCGCCAGCCTCAAAGCCGAATTGGTCGCTGCGCTGCAAGCCTTGCTGGAGCTC
GCATGA

Upstream 100 bases:

>100_bases
GCCACGCGAAATTATTCAAGCGTTTGGCAGTCTCGGCCAGTATCAGCAAGCCGTAGACGAGCTAGTGCGGCACTTATATG
CTGCATAATAAAAGGGTTGC

Downstream 100 bases:

>100_bases
ACGAACTGATTACCGCGCTCGATCAGTTAGCTGAAACGCCCCAAGCCATCAGTCGCATTCGCGAGCTTATCTTGCAATTG
GCTGTGCAAGGCCGCTTGGT

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNLTTTIKSIQDIMRKDAGVDGDAQRIGQLVWLLFLKIFDDREQELALIDPHYRSPMPEGLAWHQWAINREGITGEELLN
FVNNQLFPQLKNLAATNQAKAMIQSVFEDAYNYMKNGTLLRQVINKINEIDFNRSADRHLFNDVYEKLLSDLQAAGNAGE
YYTPRTVTQFMIEMLKPRLGESLLDPAAGTGGFLVSAVEYIRRNDVHTPSDLETLQANIRGIEKKPLPHLLGITNLILHG
INLPNLQHANTLARSYSSYGVDDQVDIIATNPPFGGQEEDGIENNFPELFRTRETADLFLVLIMRLLKPNGRAALILPDG
TLFGEGIKTRIKEELLKNCNLHTIVRLPNGVFNPYTGIKTNLLFFEKGQPTQEIWYYEHPYPAGYKSYSKTKPIRLEEFE
PERAWWINRQTNQFAWKVDIADIRANNFNLDWKNPNRQADAQTDPYQLLAEYQAATASAAQTRASLKAELVAALQALLEL
A

Sequences:

>Translated_481_residues
MNLTTTIKSIQDIMRKDAGVDGDAQRIGQLVWLLFLKIFDDREQELALIDPHYRSPMPEGLAWHQWAINREGITGEELLN
FVNNQLFPQLKNLAATNQAKAMIQSVFEDAYNYMKNGTLLRQVINKINEIDFNRSADRHLFNDVYEKLLSDLQAAGNAGE
YYTPRTVTQFMIEMLKPRLGESLLDPAAGTGGFLVSAVEYIRRNDVHTPSDLETLQANIRGIEKKPLPHLLGITNLILHG
INLPNLQHANTLARSYSSYGVDDQVDIIATNPPFGGQEEDGIENNFPELFRTRETADLFLVLIMRLLKPNGRAALILPDG
TLFGEGIKTRIKEELLKNCNLHTIVRLPNGVFNPYTGIKTNLLFFEKGQPTQEIWYYEHPYPAGYKSYSKTKPIRLEEFE
PERAWWINRQTNQFAWKVDIADIRANNFNLDWKNPNRQADAQTDPYQLLAEYQAATASAAQTRASLKAELVAALQALLEL
A
>Mature_481_residues
MNLTTTIKSIQDIMRKDAGVDGDAQRIGQLVWLLFLKIFDDREQELALIDPHYRSPMPEGLAWHQWAINREGITGEELLN
FVNNQLFPQLKNLAATNQAKAMIQSVFEDAYNYMKNGTLLRQVINKINEIDFNRSADRHLFNDVYEKLLSDLQAAGNAGE
YYTPRTVTQFMIEMLKPRLGESLLDPAAGTGGFLVSAVEYIRRNDVHTPSDLETLQANIRGIEKKPLPHLLGITNLILHG
INLPNLQHANTLARSYSSYGVDDQVDIIATNPPFGGQEEDGIENNFPELFRTRETADLFLVLIMRLLKPNGRAALILPDG
TLFGEGIKTRIKEELLKNCNLHTIVRLPNGVFNPYTGIKTNLLFFEKGQPTQEIWYYEHPYPAGYKSYSKTKPIRLEEFE
PERAWWINRQTNQFAWKVDIADIRANNFNLDWKNPNRQADAQTDPYQLLAEYQAATASAAQTRASLKAELVAALQALLEL
A

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=407, Percent_Identity=30.7125307125307, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54629; Mature: 54629

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTTTIKSIQDIMRKDAGVDGDAQRIGQLVWLLFLKIFDDREQELALIDPHYRSPMPEG
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCCCC
LAWHQWAINREGITGEELLNFVNNQLFPQLKNLAATNQAKAMIQSVFEDAYNYMKNGTLL
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RQVINKINEIDFNRSADRHLFNDVYEKLLSDLQAAGNAGEYYTPRTVTQFMIEMLKPRLG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
ESLLDPAAGTGGFLVSAVEYIRRNDVHTPSDLETLQANIRGIEKKPLPHLLGITNLILHG
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
INLPNLQHANTLARSYSSYGVDDQVDIIATNPPFGGQEEDGIENNFPELFRTRETADLFL
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLIMRLLKPNGRAALILPDGTLFGEGIKTRIKEELLKNCNLHTIVRLPNGVFNPYTGIKT
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
NLLFFEKGQPTQEIWYYEHPYPAGYKSYSKTKPIRLEEFEPERAWWINRQTNQFAWKVDI
EEEEEECCCCHHHEEEECCCCCCCCCCCCCCCCEECCCCCCCHHEEECCCCCCEEEEEEE
ADIRANNFNLDWKNPNRQADAQTDPYQLLAEYQAATASAAQTRASLKAELVAALQALLEL
EEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MNLTTTIKSIQDIMRKDAGVDGDAQRIGQLVWLLFLKIFDDREQELALIDPHYRSPMPEG
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCCCC
LAWHQWAINREGITGEELLNFVNNQLFPQLKNLAATNQAKAMIQSVFEDAYNYMKNGTLL
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RQVINKINEIDFNRSADRHLFNDVYEKLLSDLQAAGNAGEYYTPRTVTQFMIEMLKPRLG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
ESLLDPAAGTGGFLVSAVEYIRRNDVHTPSDLETLQANIRGIEKKPLPHLLGITNLILHG
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
INLPNLQHANTLARSYSSYGVDDQVDIIATNPPFGGQEEDGIENNFPELFRTRETADLFL
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLIMRLLKPNGRAALILPDGTLFGEGIKTRIKEELLKNCNLHTIVRLPNGVFNPYTGIKT
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
NLLFFEKGQPTQEIWYYEHPYPAGYKSYSKTKPIRLEEFEPERAWWINRQTNQFAWKVDI
EEEEEECCCCHHHEEEECCCCCCCCCCCCCCCCEECCCCCCCHHEEECCCCCCEEEEEEE
ADIRANNFNLDWKNPNRQADAQTDPYQLLAEYQAATASAAQTRASLKAELVAALQALLEL
EEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]