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The map label for this gene is rpoD [H]

Identifier: 159897807

GI number: 159897807

Start: 1487276

End: 1488187

Strand: Reverse

Name: rpoD [H]

Synonym: Haur_1278

Alternate gene names: 159897807

Gene position: 1488187-1487276 (Counterclockwise)

Preceding gene: 159897809

Following gene: 159897806

Centisome position: 23.45

GC content: 51.43

Gene sequence:

>912_bases
ATGATGTTGCACGCCAGCAACGAACCAGCCCTTGATGATGGGCTGCAACGTTATCTTAATGAAATTGCTGAAACTCCACT
CCTGGCAGCAGAAGAGGAGCAAAAGCTAACCCAAATTGTCCATGTAGCGCGTCAAGCTCAGCCAACCCCAGCCTGTCGAA
TTGAACGTTGCCCAAGTTGTGCCCTATTTGAGCAATGTGTAACCAAACTCAAAGAGCCAGCCCACGAGCAACTGGTGCGG
GCTAATTTACGCTTGGTCGTCAGCATCGCCAAGCGCTATCGTGGCTTTGGCCTGCCCTTCCAAGATTTAATTCAAGAGGG
CAACATTGGCCTGATGAATGCGATCGAGCGCTTTGATGCCACCAAAGGCGTGCGCTTCTCAACCTATGCTACCTGGTGGA
TTCGCCAAGCGATCACACGTTCGCTGGCCAATCAAGGTCGCTTAATTCGCTTGCCAGTCCATCGCTGGGAGCTGTTGATT
AAAGTGCGGCGGATCGAAACCAAATTACTGCAAGCGCTAGGCCGTGAACCAAGCGCCAACGAAATTAGCACTGCCTTGGG
TGTCCCTGAAGAAAAAATTTGCGATTTACTGAGCATCGCCCAAGTGCCAATCTCGCTGGCAACCCCTAAAAATGAAGAAA
CTGATATGTTGCTCGGCGATAGCTTGCCCGACGAAGATTGGGAAGACGAACTCGAATGTCTGCTGACCAGCATTGATGCT
CAATATACCCGTGCCATGCTCAACCAACTTTCTGAGCAAGAGCAAGAAGTGTTGACGCTGCGCTATGGCTTAAAAGATGG
TAAAAGCCGTTCACTCTCGGAAGTTGGCGCAGTGGTGGGCCGCACGCGCCAGCGGATTCAACAAATCGAAGCGGGTGCAT
TGCAAGTCTTGCGCACGCAGTTATCGCAATAG

Upstream 100 bases:

>100_bases
TTAGGCACCAAAGATCAGGTCACGACCTGATGTCAAGCCCTTTTGAGGTGATTAAACTAGCTATACGCCCGTCACAGCAT
CGATTTATAGGAGTGAGCCA

Downstream 100 bases:

>100_bases
CCGAGCTTGAACGATGCATAGCTATTTGATGGTCGCAGGATTACTCACGGTGCTCATGGCGTTAGCCCATAGCATTTTTG
GTGAACAATTGATCTTGCGG

Product: RNA polymerase sigma-32 subunit RpoH

Products: NA

Alternate protein names: Sigma-42 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MMLHASNEPALDDGLQRYLNEIAETPLLAAEEEQKLTQIVHVARQAQPTPACRIERCPSCALFEQCVTKLKEPAHEQLVR
ANLRLVVSIAKRYRGFGLPFQDLIQEGNIGLMNAIERFDATKGVRFSTYATWWIRQAITRSLANQGRLIRLPVHRWELLI
KVRRIETKLLQALGREPSANEISTALGVPEEKICDLLSIAQVPISLATPKNEETDMLLGDSLPDEDWEDELECLLTSIDA
QYTRAMLNQLSEQEQEVLTLRYGLKDGKSRSLSEVGAVVGRTRQRIQQIEAGALQVLRTQLSQ

Sequences:

>Translated_303_residues
MMLHASNEPALDDGLQRYLNEIAETPLLAAEEEQKLTQIVHVARQAQPTPACRIERCPSCALFEQCVTKLKEPAHEQLVR
ANLRLVVSIAKRYRGFGLPFQDLIQEGNIGLMNAIERFDATKGVRFSTYATWWIRQAITRSLANQGRLIRLPVHRWELLI
KVRRIETKLLQALGREPSANEISTALGVPEEKICDLLSIAQVPISLATPKNEETDMLLGDSLPDEDWEDELECLLTSIDA
QYTRAMLNQLSEQEQEVLTLRYGLKDGKSRSLSEVGAVVGRTRQRIQQIEAGALQVLRTQLSQ
>Mature_303_residues
MMLHASNEPALDDGLQRYLNEIAETPLLAAEEEQKLTQIVHVARQAQPTPACRIERCPSCALFEQCVTKLKEPAHEQLVR
ANLRLVVSIAKRYRGFGLPFQDLIQEGNIGLMNAIERFDATKGVRFSTYATWWIRQAITRSLANQGRLIRLPVHRWELLI
KVRRIETKLLQALGREPSANEISTALGVPEEKICDLLSIAQVPISLATPKNEETDMLLGDSLPDEDWEDELECLLTSIDA
QYTRAMLNQLSEQEQEVLTLRYGLKDGKSRSLSEVGAVVGRTRQRIQQIEAGALQVLRTQLSQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=48.4444444444444, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=37.0629370629371, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1789871, Length=309, Percent_Identity=25.5663430420712, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788231, Length=221, Percent_Identity=31.6742081447964, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34191; Mature: 34191

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLHASNEPALDDGLQRYLNEIAETPLLAAEEEQKLTQIVHVARQAQPTPACRIERCPSC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
ALFEQCVTKLKEPAHEQLVRANLRLVVSIAKRYRGFGLPFQDLIQEGNIGLMNAIERFDA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHC
TKGVRFSTYATWWIRQAITRSLANQGRLIRLPVHRWELLIKVRRIETKLLQALGREPSAN
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EISTALGVPEEKICDLLSIAQVPISLATPKNEETDMLLGDSLPDEDWEDELECLLTSIDA
HHHHHHCCCHHHHHHHHHHHHCCHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
QYTRAMLNQLSEQEQEVLTLRYGLKDGKSRSLSEVGAVVGRTRQRIQQIEAGALQVLRTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQ
HCC
>Mature Secondary Structure
MMLHASNEPALDDGLQRYLNEIAETPLLAAEEEQKLTQIVHVARQAQPTPACRIERCPSC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
ALFEQCVTKLKEPAHEQLVRANLRLVVSIAKRYRGFGLPFQDLIQEGNIGLMNAIERFDA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHC
TKGVRFSTYATWWIRQAITRSLANQGRLIRLPVHRWELLIKVRRIETKLLQALGREPSAN
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EISTALGVPEEKICDLLSIAQVPISLATPKNEETDMLLGDSLPDEDWEDELECLLTSIDA
HHHHHHCCCHHHHHHHHHHHHCCHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
QYTRAMLNQLSEQEQEVLTLRYGLKDGKSRSLSEVGAVVGRTRQRIQQIEAGALQVLRTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8914262; 12663927 [H]