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The map label for this gene is ligA

Identifier: 159897339

GI number: 159897339

Start: 917439

End: 919460

Strand: Direct

Name: ligA

Synonym: Haur_0810

Alternate gene names: 159897339

Gene position: 917439-919460 (Clockwise)

Preceding gene: 159897337

Following gene: 159897346

Centisome position: 14.46

GC content: 51.88

Gene sequence:

>2022_bases
ATGGCCGTGTCAGAGCAGACGGTTGCCCGCGCCGCAAGCTTGCGCGATGAATTGAATCTATACAATCATCATTATTATAC
GCTTGATGCACCGCTGGTCAGCGATGCTCAATACGATAGTTTATTAAATGAATTGCGGGCGATTGAGGCCGAATATCCCG
AATTACGCACCCCCGATTCGCCGACCCAACGGGTTGGTAGTGCTCCGTTGAGCAAATTTCCCAAAGTGCAGCACCCTGTG
CCAATGTTGAGCCTTGGCAATGCCTTTAATGCCGATGATTTGGCCGCGTGGCGACGACGCGCTGAACAAATTATTGGTAC
GCAGCCGATGAGCTATACCGTTGAGCCAAAAATTGATGGCTTGGCCGTGGCATTAACCTATATTAATGGGGTATTTAGCG
TTGGCGCAACCCGTGGCAACGGCGAAATTGGCGAGGATATTACCGCCAACCTACGCACAATTCGCGATGTGCCCTTGCGG
CTGCAACCAATCGACGGCCAAGCCTTGCCCGAACGCATCGAAGTGCGTGGCGAGGTCTATTTGCCTATCGAATCGTTTAA
TCAATTGAATGAACGCCAAGCCCATGCTGGCGAAAAAGTCTTTGCCAATCCACGCAATGCTGCCGCTGGATCGTTGCGTC
AGCTCGATTCAACGATTACTGCTAGCCGTCCGTTGCGCTTTTTTGCCTACGCTGTCGGCCCTTTCAGCGGCGTTGAACTC
AAAAGCCAAGCCCAAACCCTTGATACCTTGCGCACTTATGGGTTTAGCGTTAATCCCGATACGCGGCTTTTTGCTGATTT
TGAGGCGGTAATCGAATATTGCCACGAGTGGATGAGCCGCCGTGAATCGCTAAGCTACGAAGTTGATGGCGTGGTAGTTA
AAATTAATGATTTTGCCATGCAACGTGAATTGGGCGTGGTTGGTCGTGATCCACGCTGGGCGATTGCCTATAAATTTCCA
GCTCGCGAAGAAACCACCACCTTGCTCAATATTGTGATCAATGTTGGTCGCACTGGTAAATTGATTCCCAATGCTGTGCT
CGAACCTGTCAGTTTGGGCGGCACGACGGTGCAGCATGCCTCGTTGCACAACGCCGATTACATCATCAGCCGCGATATTC
GCATTGGCGATCGGGTTGTGGTCAAACGGGCTGGCGATGTGATTCCCTATGTGATTGGGCCAATCGTTGAGGCTCGCACT
GGCGACGAGCGAGTTTGGCCAGCGCCAACTCATTGTCCAACTTGTGGTCAGCCAGTCGAGCAAATTGGCGATGAAGTTGA
TATTTATTGCGTCAATAATACTTGTCCTGCGCGTTTGATTCGTTCAATCGAACATTGGGTCAGCCGTGGCGCGATGGATA
TTGTGGGCATGGGCGAGCGCCAAGCCAGCCAATTTGTCGAAATGGGCTTGATCAAATCGATTCCTGATATTTATCGTTTG
ACGGTTGATAGCTTTGGGGGGCGTGAAGGCTATGGCGAACGGCGCGTCGCTAATTTGCTGAATGCGATCGAAGAATCCAA
GCGACGCCCGCTTGATCGTGTCATCACCGCTTTGGGGATTAACGGAGTTGGAACGGTGGCGGCGGCGGATTTAGCCCGCT
ATTTCCGTTCATTGCCAGCCTTAGCCCAAGCCACGATTGAGCAATTGACCGCGATTGAGGGGATTGGTGGCAGCACCGCC
CAAAGCGTGGTCGATTTCTTCAATACGCCAGCCAACCAACAATTAATCGCCGAATTATTGGCTTTAGGCCTCAAAGCCGA
GCCTAGCGAAGTTGCTGAATTGCAGAGTGATCGTTTGGCGGGCAAAAGTTTTGTGATCACTGGAACCTTGCCTGGCATTA
GCCGCGAAGCCGCTCAAGCCTTGATCGAAGCCCATGGCGGCAAGGTTGGCGGTAGCGTCAGCAAGAAAACTGATTATTTG
CTGGCAGGCGAGGCAGCTGGCTCGAAATTGACCAAAGCCCAAAGTTTAGGCGTAAAAGTGCTGAGCATGGATGAGTTGCA
TGCGCTACTGGTCGATGAATAG

Upstream 100 bases:

>100_bases
GGTTAAGGTTTAGCAACATCAAACGATTCAAAATTGTGGCTAGATTTGACACAGACACCCGTTATAATACATCTGTTCGA
TTAAATTGGAGGATGCATGC

Downstream 100 bases:

>100_bases
AGCCTGAGCTTTGGTTGATCCACGAAGAGCACGAAGTTACACGAAGGGCGTAAAGGCTGAAACCGCGAAGAACGCGACGA
TCGCGAAGGATGTTAAGTTT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MAVSEQTVARAASLRDELNLYNHHYYTLDAPLVSDAQYDSLLNELRAIEAEYPELRTPDSPTQRVGSAPLSKFPKVQHPV
PMLSLGNAFNADDLAAWRRRAEQIIGTQPMSYTVEPKIDGLAVALTYINGVFSVGATRGNGEIGEDITANLRTIRDVPLR
LQPIDGQALPERIEVRGEVYLPIESFNQLNERQAHAGEKVFANPRNAAAGSLRQLDSTITASRPLRFFAYAVGPFSGVEL
KSQAQTLDTLRTYGFSVNPDTRLFADFEAVIEYCHEWMSRRESLSYEVDGVVVKINDFAMQRELGVVGRDPRWAIAYKFP
AREETTTLLNIVINVGRTGKLIPNAVLEPVSLGGTTVQHASLHNADYIISRDIRIGDRVVVKRAGDVIPYVIGPIVEART
GDERVWPAPTHCPTCGQPVEQIGDEVDIYCVNNTCPARLIRSIEHWVSRGAMDIVGMGERQASQFVEMGLIKSIPDIYRL
TVDSFGGREGYGERRVANLLNAIEESKRRPLDRVITALGINGVGTVAAADLARYFRSLPALAQATIEQLTAIEGIGGSTA
QSVVDFFNTPANQQLIAELLALGLKAEPSEVAELQSDRLAGKSFVITGTLPGISREAAQALIEAHGGKVGGSVSKKTDYL
LAGEAAGSKLTKAQSLGVKVLSMDELHALLVDE

Sequences:

>Translated_673_residues
MAVSEQTVARAASLRDELNLYNHHYYTLDAPLVSDAQYDSLLNELRAIEAEYPELRTPDSPTQRVGSAPLSKFPKVQHPV
PMLSLGNAFNADDLAAWRRRAEQIIGTQPMSYTVEPKIDGLAVALTYINGVFSVGATRGNGEIGEDITANLRTIRDVPLR
LQPIDGQALPERIEVRGEVYLPIESFNQLNERQAHAGEKVFANPRNAAAGSLRQLDSTITASRPLRFFAYAVGPFSGVEL
KSQAQTLDTLRTYGFSVNPDTRLFADFEAVIEYCHEWMSRRESLSYEVDGVVVKINDFAMQRELGVVGRDPRWAIAYKFP
AREETTTLLNIVINVGRTGKLIPNAVLEPVSLGGTTVQHASLHNADYIISRDIRIGDRVVVKRAGDVIPYVIGPIVEART
GDERVWPAPTHCPTCGQPVEQIGDEVDIYCVNNTCPARLIRSIEHWVSRGAMDIVGMGERQASQFVEMGLIKSIPDIYRL
TVDSFGGREGYGERRVANLLNAIEESKRRPLDRVITALGINGVGTVAAADLARYFRSLPALAQATIEQLTAIEGIGGSTA
QSVVDFFNTPANQQLIAELLALGLKAEPSEVAELQSDRLAGKSFVITGTLPGISREAAQALIEAHGGKVGGSVSKKTDYL
LAGEAAGSKLTKAQSLGVKVLSMDELHALLVDE
>Mature_672_residues
AVSEQTVARAASLRDELNLYNHHYYTLDAPLVSDAQYDSLLNELRAIEAEYPELRTPDSPTQRVGSAPLSKFPKVQHPVP
MLSLGNAFNADDLAAWRRRAEQIIGTQPMSYTVEPKIDGLAVALTYINGVFSVGATRGNGEIGEDITANLRTIRDVPLRL
QPIDGQALPERIEVRGEVYLPIESFNQLNERQAHAGEKVFANPRNAAAGSLRQLDSTITASRPLRFFAYAVGPFSGVELK
SQAQTLDTLRTYGFSVNPDTRLFADFEAVIEYCHEWMSRRESLSYEVDGVVVKINDFAMQRELGVVGRDPRWAIAYKFPA
REETTTLLNIVINVGRTGKLIPNAVLEPVSLGGTTVQHASLHNADYIISRDIRIGDRVVVKRAGDVIPYVIGPIVEARTG
DERVWPAPTHCPTCGQPVEQIGDEVDIYCVNNTCPARLIRSIEHWVSRGAMDIVGMGERQASQFVEMGLIKSIPDIYRLT
VDSFGGREGYGERRVANLLNAIEESKRRPLDRVITALGINGVGTVAAADLARYFRSLPALAQATIEQLTAIEGIGGSTAQ
SVVDFFNTPANQQLIAELLALGLKAEPSEVAELQSDRLAGKSFVITGTLPGISREAAQALIEAHGGKVGGSVSKKTDYLL
AGEAAGSKLTKAQSLGVKVLSMDELHALLVDE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=47.2527472527472, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788750, Length=663, Percent_Identity=45.5505279034691, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI87082305, Length=557, Percent_Identity=24.5960502692998, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_HERA2 (A9AXP4)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001543586.1
- ProteinModelPortal:   A9AXP4
- GeneID:   5732710
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0810
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK975376
- BioCyc:   HAUR316274:HAUR_0810-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73338; Mature: 73207

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 178-178 BINDING 294-294 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSEQTVARAASLRDELNLYNHHYYTLDAPLVSDAQYDSLLNELRAIEAEYPELRTPDS
CCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PTQRVGSAPLSKFPKVQHPVPMLSLGNAFNADDLAAWRRRAEQIIGTQPMSYTVEPKIDG
HHHHHCCCCHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
LAVALTYINGVFSVGATRGNGEIGEDITANLRTIRDVPLRLQPIDGQALPERIEVRGEVY
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHEECCEEE
LPIESFNQLNERQAHAGEKVFANPRNAAAGSLRQLDSTITASRPLRFFAYAVGPFSGVEL
EEHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
KSQAQTLDTLRTYGFSVNPDTRLFADFEAVIEYCHEWMSRRESLSYEVDGVVVKINDFAM
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEHHHH
QRELGVVGRDPRWAIAYKFPAREETTTLLNIVINVGRTGKLIPNAVLEPVSLGGTTVQHA
HHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEHH
SLHNADYIISRDIRIGDRVVVKRAGDVIPYVIGPIVEARTGDERVWPAPTHCPTCGQPVE
HCCCCCEEEECCCCCCCEEEEHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
QIGDEVDIYCVNNTCPARLIRSIEHWVSRGAMDIVGMGERQASQFVEMGLIKSIPDIYRL
HCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHH
TVDSFGGREGYGERRVANLLNAIEESKRRPLDRVITALGINGVGTVAAADLARYFRSLPA
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LAQATIEQLTAIEGIGGSTAQSVVDFFNTPANQQLIAELLALGLKAEPSEVAELQSDRLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GKSFVITGTLPGISREAAQALIEAHGGKVGGSVSKKTDYLLAGEAAGSKLTKAQSLGVKV
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCEEE
LSMDELHALLVDE
EEHHHHHHHHCCC
>Mature Secondary Structure 
AVSEQTVARAASLRDELNLYNHHYYTLDAPLVSDAQYDSLLNELRAIEAEYPELRTPDS
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PTQRVGSAPLSKFPKVQHPVPMLSLGNAFNADDLAAWRRRAEQIIGTQPMSYTVEPKIDG
HHHHHCCCCHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
LAVALTYINGVFSVGATRGNGEIGEDITANLRTIRDVPLRLQPIDGQALPERIEVRGEVY
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHEECCEEE
LPIESFNQLNERQAHAGEKVFANPRNAAAGSLRQLDSTITASRPLRFFAYAVGPFSGVEL
EEHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCH
KSQAQTLDTLRTYGFSVNPDTRLFADFEAVIEYCHEWMSRRESLSYEVDGVVVKINDFAM
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEHHHH
QRELGVVGRDPRWAIAYKFPAREETTTLLNIVINVGRTGKLIPNAVLEPVSLGGTTVQHA
HHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEHH
SLHNADYIISRDIRIGDRVVVKRAGDVIPYVIGPIVEARTGDERVWPAPTHCPTCGQPVE
HCCCCCEEEECCCCCCCEEEEHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
QIGDEVDIYCVNNTCPARLIRSIEHWVSRGAMDIVGMGERQASQFVEMGLIKSIPDIYRL
HCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHH
TVDSFGGREGYGERRVANLLNAIEESKRRPLDRVITALGINGVGTVAAADLARYFRSLPA
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LAQATIEQLTAIEGIGGSTAQSVVDFFNTPANQQLIAELLALGLKAEPSEVAELQSDRLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GKSFVITGTLPGISREAAQALIEAHGGKVGGSVSKKTDYLLAGEAAGSKLTKAQSLGVKV
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCEEE
LSMDELHALLVDE
EEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA