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The map label for this gene is lipA1 [H]

Identifier: 159896716

GI number: 159896716

Start: 211951

End: 212871

Strand: Reverse

Name: lipA1 [H]

Synonym: Haur_0183

Alternate gene names: 159896716

Gene position: 212871-211951 (Counterclockwise)

Preceding gene: 159896730

Following gene: 159896715

Centisome position: 3.35

GC content: 51.03

Gene sequence:

>921_bases
ATGCCATCAACACCGCATCGTCAGCGTAAACCAGAGTGGTTACGCAAACCTGTGCTTTCACCAGAGTTGCGTAATGTCCG
TCAATTACTGCGTGATTTGAATCTGAATACTGTGTGTGAAGAAGCATCGTGCCCCAACATTGCCGAATGCTTTGGCCATG
GCACTGCTACATTTATGATCGGCGGCGATCGTTGTACTCGACGTTGCCATTATTGCGATGTCACGACTGGCAAGCCTTTT
CCGCTTGATCCACGCGAGCCGAGCAATGTCGCCGAAGCCGCTGCTCGCTTGGGCTTGAAGTATGTGGTAGTTACCGCCGT
TGCTCGCGATGATGTCAAGGATGGCGGCGCTCGCCACTTTGCCCGCACCATCAGCGCGATTCGTCAACGCTTACCCGAAG
CCGAAGTTGAGGTCTTGATTCCTGATTTCAAAGGCGAGGCCGAAGCGCTCAAAACGGTATTGGATGCCGAACCACATGTG
CTCAATCACAATATCGAAACTGTTCGTAGCGTGTTTAAGAGTGTGCGGCCCCAAGGCGATTATGATCGATCGCTCGAATT
ATTAGCTCGTAGTCGTGAGTTTGCCCCGCAGATTACAACTAAATCGGGGTTTATGGTGGGTTTAGGCGAAACAACCGACG
AAATCTACCAAACTATGGATGATCTCAAGCATGTTGGGGTTGAACTGGTCACGATTGGCCAATATTTACAACCATCACTG
AACCATGTGGCAGTGAGCGAATATATTCCTCCCGAGCGTTATGGCTTGTATCGCGACCATGGCCAGCAATTGGGCTTTCG
AGCAACCTTTGCTGGGCCATTCATTCGATCATCATTCCACGCTGGGGAATTACAACACCATCAACCAAGCAGCGACGTGG
CTAGCCCAGTGCAAGAATGGATCGCACTCGAACCACGTTAG

Upstream 100 bases:

>100_bases
AATAGCGAGTGAGGGTTATCGCTGGCTTAAAGGCAAGCTGGTACCACATTCGTCTGCCGTCACTTGCAATGCTGCATGTG
CTGCTTAACGTGAGGTTGGT

Downstream 100 bases:

>100_bases
ATTATAGATAGAAGGAGTTCCTATGTCGTCCGTGCATGAAACGCATTTATTGGCTGAAGGTATTACTGCCGCCAAAGCAG
GCCAACGTGCAGCAGCCCGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPSTPHRQRKPEWLRKPVLSPELRNVRQLLRDLNLNTVCEEASCPNIAECFGHGTATFMIGGDRCTRRCHYCDVTTGKPF
PLDPREPSNVAEAAARLGLKYVVVTAVARDDVKDGGARHFARTISAIRQRLPEAEVEVLIPDFKGEAEALKTVLDAEPHV
LNHNIETVRSVFKSVRPQGDYDRSLELLARSREFAPQITTKSGFMVGLGETTDEIYQTMDDLKHVGVELVTIGQYLQPSL
NHVAVSEYIPPERYGLYRDHGQQLGFRATFAGPFIRSSFHAGELQHHQPSSDVASPVQEWIALEPR

Sequences:

>Translated_306_residues
MPSTPHRQRKPEWLRKPVLSPELRNVRQLLRDLNLNTVCEEASCPNIAECFGHGTATFMIGGDRCTRRCHYCDVTTGKPF
PLDPREPSNVAEAAARLGLKYVVVTAVARDDVKDGGARHFARTISAIRQRLPEAEVEVLIPDFKGEAEALKTVLDAEPHV
LNHNIETVRSVFKSVRPQGDYDRSLELLARSREFAPQITTKSGFMVGLGETTDEIYQTMDDLKHVGVELVTIGQYLQPSL
NHVAVSEYIPPERYGLYRDHGQQLGFRATFAGPFIRSSFHAGELQHHQPSSDVASPVQEWIALEPR
>Mature_305_residues
PSTPHRQRKPEWLRKPVLSPELRNVRQLLRDLNLNTVCEEASCPNIAECFGHGTATFMIGGDRCTRRCHYCDVTTGKPFP
LDPREPSNVAEAAARLGLKYVVVTAVARDDVKDGGARHFARTISAIRQRLPEAEVEVLIPDFKGEAEALKTVLDAEPHVL
NHNIETVRSVFKSVRPQGDYDRSLELLARSREFAPQITTKSGFMVGLGETTDEIYQTMDDLKHVGVELVTIGQYLQPSLN
HVAVSEYIPPERYGLYRDHGQQLGFRATFAGPFIRSSFHAGELQHHQPSSDVASPVQEWIALEPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=44.2508710801394, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=43.7751004016064, Blast_Score=221, Evalue=9e-58,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=48.9130434782609, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=290, Percent_Identity=41.7241379310345, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=41.9243986254296, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=44.9477351916376, Blast_Score=260, Evalue=9e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34288; Mature: 34157

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTPHRQRKPEWLRKPVLSPELRNVRQLLRDLNLNTVCEEASCPNIAECFGHGTATFMI
CCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHCCCCEEEEE
GGDRCTRRCHYCDVTTGKPFPLDPREPSNVAEAAARLGLKYVVVTAVARDDVKDGGARHF
CCHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHH
ARTISAIRQRLPEAEVEVLIPDFKGEAEALKTVLDAEPHVLNHNIETVRSVFKSVRPQGD
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCCC
YDRSLELLARSREFAPQITTKSGFMVGLGETTDEIYQTMDDLKHVGVELVTIGQYLQPSL
HHHHHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCHHEEHHHHHCCCH
NHVAVSEYIPPERYGLYRDHGQQLGFRATFAGPFIRSSFHAGELQHHQPSSDVASPVQEW
HHEEHHHCCCHHHCCCHHHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IALEPR
HCCCCC
>Mature Secondary Structure 
PSTPHRQRKPEWLRKPVLSPELRNVRQLLRDLNLNTVCEEASCPNIAECFGHGTATFMI
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHCCCCEEEEE
GGDRCTRRCHYCDVTTGKPFPLDPREPSNVAEAAARLGLKYVVVTAVARDDVKDGGARHF
CCHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHH
ARTISAIRQRLPEAEVEVLIPDFKGEAEALKTVLDAEPHVLNHNIETVRSVFKSVRPQGD
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCCC
YDRSLELLARSREFAPQITTKSGFMVGLGETTDEIYQTMDDLKHVGVELVTIGQYLQPSL
HHHHHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCHHEEHHHHHCCCH
NHVAVSEYIPPERYGLYRDHGQQLGFRATFAGPFIRSSFHAGELQHHQPSSDVASPVQEW
HHEEHHHCCCHHHCCCHHHHHHHCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IALEPR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]