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The map label for this gene is hisF

Identifier: 159896691

GI number: 159896691

Start: 188932

End: 189723

Strand: Direct

Name: hisF

Synonym: Haur_0158

Alternate gene names: 159896691

Gene position: 188932-189723 (Clockwise)

Preceding gene: 159896690

Following gene: 159896692

Centisome position: 2.98

GC content: 53.28

Gene sequence:

>792_bases
ATGCTGACACGGCGAATTATTCCTTGTTTGGATGTGAAGGCTGGGCGTGTAGTCAAGGGCATCAGCTTTCTCAACCATCG
CGATGCTGGTGATCCAGTGCAATTAGCAGCCTTCTACAACGAATCGGGCGCTGATGAATTGGTGTTTTACGATATTACCG
CTTCATCCGATGAGCGCAATATTATGGTCGAAGTGGTTGAGAATGTGGCACGGCAGGTCTTTATTCCGCTAACGGTTGGT
GGTGGCATTCGCACGGTCGATGATATGTATCGAATGTTGCGGGCTGGCGCGGATAAAGTTTCGATCAATACCGCTGCGGT
GCTCAACCCGCAATTAATCGAAGATGGAGCCAAGCGTTTCGGCTCACAGTGCATTGTGCTTTCGATGGATGCGCGTCGGA
TCAACGAGCCAGGCCAACCCAGCCAATGGAATGTTTTCACCCATACTGGGCGCGATCCACGGCCAACCGGCTTGGATGCA
ATTGAATGGGCCAAACGAGTGGTCGATTTGGGTGCTGGCGAGTTGGTGATCAACAGCATGGATGCCGATGGTACTGGCGC
GGGCTACGATAACGAATTATTATCGGCGATTAGCCAGCAAGTTGGCGTGCCTGTGATTGCCTCTGGTGGTGCTGGCAAGC
CCGAACATTTGCTCGCCGCATTGCACGAAGGCAAAGCCGATGCCGTCTTGGCTGCCTCGATCTTCCACTTTGGCACCTAC
ACGGTCGAAGCCGTCAAAGAATATTTGGCCAATCAAGGCATTCCAATGCGCCGCACGCCGAGGCCTGTATGA

Upstream 100 bases:

>100_bases
GGCGCGAAGTGCAAGCGCTGAGCGATATGTTGGCGGCAATTCAAGGCCGTTTGCAAATTGCCCAAAGCGAAATTTTGGAA
CGTGGAAGCGAGATCTAAGC

Downstream 100 bases:

>100_bases
TCATTAGCATTCGTTTATTTGCTGGCTTGCGTGAAGCACTCAATCAATCAAGTCTGAATTTGGATGTGGCTGAGACTGCG
ACAGTTGGCACAGCGCTGGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLTRRIIPCLDVKAGRVVKGISFLNHRDAGDPVQLAAFYNESGADELVFYDITASSDERNIMVEVVENVARQVFIPLTVG
GGIRTVDDMYRMLRAGADKVSINTAAVLNPQLIEDGAKRFGSQCIVLSMDARRINEPGQPSQWNVFTHTGRDPRPTGLDA
IEWAKRVVDLGAGELVINSMDADGTGAGYDNELLSAISQQVGVPVIASGGAGKPEHLLAALHEGKADAVLAASIFHFGTY
TVEAVKEYLANQGIPMRRTPRPV

Sequences:

>Translated_263_residues
MLTRRIIPCLDVKAGRVVKGISFLNHRDAGDPVQLAAFYNESGADELVFYDITASSDERNIMVEVVENVARQVFIPLTVG
GGIRTVDDMYRMLRAGADKVSINTAAVLNPQLIEDGAKRFGSQCIVLSMDARRINEPGQPSQWNVFTHTGRDPRPTGLDA
IEWAKRVVDLGAGELVINSMDADGTGAGYDNELLSAISQQVGVPVIASGGAGKPEHLLAALHEGKADAVLAASIFHFGTY
TVEAVKEYLANQGIPMRRTPRPV
>Mature_263_residues
MLTRRIIPCLDVKAGRVVKGISFLNHRDAGDPVQLAAFYNESGADELVFYDITASSDERNIMVEVVENVARQVFIPLTVG
GGIRTVDDMYRMLRAGADKVSINTAAVLNPQLIEDGAKRFGSQCIVLSMDARRINEPGQPSQWNVFTHTGRDPRPTGLDA
IEWAKRVVDLGAGELVINSMDADGTGAGYDNELLSAISQQVGVPVIASGGAGKPEHLLAALHEGKADAVLAASIFHFGTY
TVEAVKEYLANQGIPMRRTPRPV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=47.1042471042471, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI87082028, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=36.8253968253968, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_HERA2 (A9B5I5)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001542938.1
- ProteinModelPortal:   A9B5I5
- SMR:   A9B5I5
- GeneID:   5732067
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0158
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   HAUR316274:HAUR_0158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28342; Mature: 28342

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRIIPCLDVKAGRVVKGISFLNHRDAGDPVQLAAFYNESGADELVFYDITASSDERN
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCH
IMVEVVENVARQVFIPLTVGGGIRTVDDMYRMLRAGADKVSINTAAVLNPQLIEDGAKRF
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEEECEEEECCHHHHHHHHHHH
GSQCIVLSMDARRINEPGQPSQWNVFTHTGRDPRPTGLDAIEWAKRVVDLGAGELVINSM
CCEEEEEEECHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECC
DADGTGAGYDNELLSAISQQVGVPVIASGGAGKPEHLLAALHEGKADAVLAASIFHFGTY
CCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
TVEAVKEYLANQGIPMRRTPRPV
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLTRRIIPCLDVKAGRVVKGISFLNHRDAGDPVQLAAFYNESGADELVFYDITASSDERN
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCH
IMVEVVENVARQVFIPLTVGGGIRTVDDMYRMLRAGADKVSINTAAVLNPQLIEDGAKRF
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEEECEEEECCHHHHHHHHHHH
GSQCIVLSMDARRINEPGQPSQWNVFTHTGRDPRPTGLDAIEWAKRVVDLGAGELVINSM
CCEEEEEEECHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECC
DADGTGAGYDNELLSAISQQVGVPVIASGGAGKPEHLLAALHEGKADAVLAASIFHFGTY
CCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
TVEAVKEYLANQGIPMRRTPRPV
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA