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The map label for this gene is dnaK [H]

Identifier: 159896686

GI number: 159896686

Start: 183085

End: 184431

Strand: Reverse

Name: dnaK [H]

Synonym: Haur_0153

Alternate gene names: 159896686

Gene position: 184431-183085 (Counterclockwise)

Preceding gene: 159896699

Following gene: 159896685

Centisome position: 2.91

GC content: 52.93

Gene sequence:

>1347_bases
ATGCGGGTAGGCTTAGATTTCGGAACAACTAATACGACCGCAGCAATTTACGACGGCGCAAACGTAAGCTTGGTTCCGCT
AGACTCCGTAGCGGCAAATCCCAATGTGTTACGCACTGCCTTATTTATCACGCCCGAAGGCCAGCAACGGATTGGCCGCG
CGGCAATTAATGCCTTTACTGAGGGCAACGTTGGCCGCGAAATCGTCTACGAGCGACGCTATCTTGGCACAGTTACCAAC
ACCTATGCAGGGGTTGGCACAATCACGCAGGCCGTCGAAGGCTTGGTCGATAGCAACCCACCAGGCCGTTTATTTCAATC
GATCAAAACCATGCTGCGCGATCCATCCTACATCAAAACCAATGTGTTTGGCCAAGAAACGACCCTCGAAGAATTGATCG
CCAAAATCCTACGGGCAATTCGCCGTGAGATCGAACGTTTCACTGGCGAGAAAATTAGCGCGATTACAGTTGGGCGGCCT
GTGCACTATGCCAGCACAGCGGAAGGCGATGCGTTGGCGATTCGGCGGATGCGTGAGGCCTGCGAATTGGCCGATTTGCC
AAATGTGAGTTTTATGCCCGAACCTGTCGCGGCAGCCCATGCCTTTGCTGCCAGTCAACGCGAACGCCGCAATATTATCG
TCTTCGACTTTGGCGGCGGGACGCTCGACGTAACCGTAATGGCAATTGATGGGGCCAAACGCCAAGTGCTAGCGACCGAT
GGTGTGCCAATTGGCGGCGATGTGCTCGATAGCAAAATTGTTACGGGAGCCTTACGCCAATATTTTGGTGATGGCGCTCG
ACTTGGCCCACGCAAATTGCCCTTGCCCGCTGCTTTGATGGAGCACCTCGACAATTGGCAAAATATTCTCGAAATGCACA
CACCCAAAATGCTCGACATCATCGAAGAATCGGTGCGCACCAGTGATAAGCCCAAAGAACTCAAGGCCTTGCGCTCATTA
GTCCGCGAAAATTATGGGTTGGTGCTGTATGAGCATGCGGAAGAGGCTAAACGGCGGCTTTCCAGCGAACGCCAAGTTGA
AATTAGCATGCATGTTAAAGATATTGATTTTGATCATTTGCTGCCACGCTTTGAATTTGAGCGCCTCGTTGGCCCCGAAG
CTCGTGCCATCGGTGATTGTGTTGATCGCACGGTTCGCGCGGCTGGCTTGAGCCACGAGCAAATTGATGTAGTGTTGCGC
ACCGGCGGTTCTTCGCGGATTCCGCGTTTTATCAAATTATTAGCCGACCGCTTTACCGAAGATCGGCTGCGTGAGCAAGA
TCCATTTACCAGCGTCGGGGCAGGCTTAGCCGTCGCTGCCTACGAAGGTTTGGGCGAAGCTAATTAA

Upstream 100 bases:

>100_bases
GCTTTGTTACAATCAATGTTCTGCACCAGCCGCCCCCCAGTTTTGGGTTTGGGCGGCATTTCGCCGTGCAACTCATCCAG
CTTAATTAGGAGGCATTGCA

Downstream 100 bases:

>100_bases
ATGGTGGTAGTTGCCAAGGCTTGGTATCAAAACTGCCAAGCCTTAGCGATCTCAGCTATAATGCAACTAGCTTTAGTCGG
GAGTATGCGTGATGGTCAAA

Product: molecular chaperone-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MRVGLDFGTTNTTAAIYDGANVSLVPLDSVAANPNVLRTALFITPEGQQRIGRAAINAFTEGNVGREIVYERRYLGTVTN
TYAGVGTITQAVEGLVDSNPPGRLFQSIKTMLRDPSYIKTNVFGQETTLEELIAKILRAIRREIERFTGEKISAITVGRP
VHYASTAEGDALAIRRMREACELADLPNVSFMPEPVAAAHAFAASQRERRNIIVFDFGGGTLDVTVMAIDGAKRQVLATD
GVPIGGDVLDSKIVTGALRQYFGDGARLGPRKLPLPAALMEHLDNWQNILEMHTPKMLDIIEESVRTSDKPKELKALRSL
VRENYGLVLYEHAEEAKRRLSSERQVEISMHVKDIDFDHLLPRFEFERLVGPEARAIGDCVDRTVRAAGLSHEQIDVVLR
TGGSSRIPRFIKLLADRFTEDRLREQDPFTSVGAGLAVAAYEGLGEAN

Sequences:

>Translated_448_residues
MRVGLDFGTTNTTAAIYDGANVSLVPLDSVAANPNVLRTALFITPEGQQRIGRAAINAFTEGNVGREIVYERRYLGTVTN
TYAGVGTITQAVEGLVDSNPPGRLFQSIKTMLRDPSYIKTNVFGQETTLEELIAKILRAIRREIERFTGEKISAITVGRP
VHYASTAEGDALAIRRMREACELADLPNVSFMPEPVAAAHAFAASQRERRNIIVFDFGGGTLDVTVMAIDGAKRQVLATD
GVPIGGDVLDSKIVTGALRQYFGDGARLGPRKLPLPAALMEHLDNWQNILEMHTPKMLDIIEESVRTSDKPKELKALRSL
VRENYGLVLYEHAEEAKRRLSSERQVEISMHVKDIDFDHLLPRFEFERLVGPEARAIGDCVDRTVRAAGLSHEQIDVVLR
TGGSSRIPRFIKLLADRFTEDRLREQDPFTSVGAGLAVAAYEGLGEAN
>Mature_448_residues
MRVGLDFGTTNTTAAIYDGANVSLVPLDSVAANPNVLRTALFITPEGQQRIGRAAINAFTEGNVGREIVYERRYLGTVTN
TYAGVGTITQAVEGLVDSNPPGRLFQSIKTMLRDPSYIKTNVFGQETTLEELIAKILRAIRREIERFTGEKISAITVGRP
VHYASTAEGDALAIRRMREACELADLPNVSFMPEPVAAAHAFAASQRERRNIIVFDFGGGTLDVTVMAIDGAKRQVLATD
GVPIGGDVLDSKIVTGALRQYFGDGARLGPRKLPLPAALMEHLDNWQNILEMHTPKMLDIIEESVRTSDKPKELKALRSL
VRENYGLVLYEHAEEAKRRLSSERQVEISMHVKDIDFDHLLPRFEFERLVGPEARAIGDCVDRTVRAAGLSHEQIDVVLR
TGGSSRIPRFIKLLADRFTEDRLREQDPFTSVGAGLAVAAYEGLGEAN

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI16507237, Length=446, Percent_Identity=26.0089686098655, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI87082035, Length=469, Percent_Identity=23.454157782516, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786196, Length=466, Percent_Identity=24.2489270386266, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17534771, Length=444, Percent_Identity=25.6756756756757, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17568549, Length=441, Percent_Identity=25.3968253968254, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534015, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17534013, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71984444, Length=464, Percent_Identity=24.5689655172414, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320950, Length=319, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322426, Length=318, Percent_Identity=27.9874213836478, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641406, Length=446, Percent_Identity=24.2152466367713, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24641408, Length=446, Percent_Identity=24.2152466367713, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24641402, Length=446, Percent_Identity=24.2152466367713, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24641404, Length=446, Percent_Identity=24.2152466367713, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28571678, Length=318, Percent_Identity=26.1006289308176, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 49282; Mature: 49282

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGLDFGTTNTTAAIYDGANVSLVPLDSVAANPNVLRTALFITPEGQQRIGRAAINAFT
CEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHC
EGNVGREIVYERRYLGTVTNTYAGVGTITQAVEGLVDSNPPGRLFQSIKTMLRDPSYIKT
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEE
NVFGQETTLEELIAKILRAIRREIERFTGEKISAITVGRPVHYASTAEGDALAIRRMREA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHH
CELADLPNVSFMPEPVAAAHAFAASQRERRNIIVFDFGGGTLDVTVMAIDGAKRQVLATD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCEEEEEC
GVPIGGDVLDSKIVTGALRQYFGDGARLGPRKLPLPAALMEHLDNWQNILEMHTPKMLDI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
IEESVRTSDKPKELKALRSLVRENYGLVLYEHAEEAKRRLSSERQVEISMHVKDIDFDHL
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEEEEEEEEECCHHHH
LPRFEFERLVGPEARAIGDCVDRTVRAAGLSHEQIDVVLRTGGSSRIPRFIKLLADRFTE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHH
DRLREQDPFTSVGAGLAVAAYEGLGEAN
HHHHCCCCCHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MRVGLDFGTTNTTAAIYDGANVSLVPLDSVAANPNVLRTALFITPEGQQRIGRAAINAFT
CEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHC
EGNVGREIVYERRYLGTVTNTYAGVGTITQAVEGLVDSNPPGRLFQSIKTMLRDPSYIKT
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEE
NVFGQETTLEELIAKILRAIRREIERFTGEKISAITVGRPVHYASTAEGDALAIRRMREA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHH
CELADLPNVSFMPEPVAAAHAFAASQRERRNIIVFDFGGGTLDVTVMAIDGAKRQVLATD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCEEEEEC
GVPIGGDVLDSKIVTGALRQYFGDGARLGPRKLPLPAALMEHLDNWQNILEMHTPKMLDI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
IEESVRTSDKPKELKALRSLVRENYGLVLYEHAEEAKRRLSSERQVEISMHVKDIDFDHL
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCEEEEEEEEEECCHHHH
LPRFEFERLVGPEARAIGDCVDRTVRAAGLSHEQIDVVLRTGGSSRIPRFIKLLADRFTE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHH
DRLREQDPFTSVGAGLAVAAYEGLGEAN
HHHHCCCCCHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA