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The map label for this gene is ygcM [C]

Identifier: 159896663

GI number: 159896663

Start: 161241

End: 162062

Strand: Direct

Name: ygcM [C]

Synonym: Haur_0130

Alternate gene names: 159896663

Gene position: 161241-162062 (Clockwise)

Preceding gene: 159896662

Following gene: 159896664

Centisome position: 2.54

GC content: 46.59

Gene sequence:

>822_bases
ATGGATGTTTTTGCAACACGGCGCTTTAATTTTTCGGCTTCGCATCGCTATTGGCGCGAAGAATGGAGCCTAGAACAAAA
TGAGGCGGTGTTCGGCAAATGTACCAATCGCTATGGTCATGGTCACAACTACGAGTTATTTGTGACGGTTGCTGGGGCGG
TTGACCCGATCACTGGGATGGTTATGAATATGGTTGAGTTAAAGCGCCTCGTAACAATGGTGTTAGACCAGTTTGATCAT
AAACATCTGAATGAGGATACCCCCTACTTTCGTGAAGTGATTCCGACAACCGAAAATCTGGTACGGGTTTTGTGGGGCTT
GATCGAGCCGCAATTGCCTAAAGGAGTACGTTTAGCCAAATTACGTTTGTATGAAAATAGCGATTTGTATGCTGAATATT
TTGGGCGGCAACAGCAGGCGACATTTAATCGGCGCTATGAATTTTCGGCGGCGCATCGCTTGCATGCCCAAAGCTTAACT
GATGAAGCGAATCGTGAAATCTATGGAAAGTGTAATAATCCCAATGGTCATGGTCATAATTATCAACTAGAGGTTACGGT
TGATGGCCTGATTGATGCCCAAACCGGCATGGTGATTGATTTGGTGGATATGGATCGGCGCGTGCAGAGTGTGCTTGACA
ACTGGGATCATCGTCATCTTGATTATCAGGTTGCCGAATTTGCCGAACAGCCCTCGACTGCTGAAAATATTGTGGTGGTG
CTGTGGCAACGCCTCGAAGCCTTATTTGGCTCGCAATTAAAGCATTTGCGGCTGTGGGAAACCGCCAATAATGTGTTTGA
TTACCCGCTCAGCCAGCATTGA

Upstream 100 bases:

>100_bases
ATACTTAAAAAACATTGAGTAGCAAGAATTTCAATGGCATAATAAAAACTACGTGATCGTTAGCTGGGGGATTGGTGGTT
AAAATAAGGATGAATTGCCC

Downstream 100 bases:

>100_bases
GCAGCGGCAACCCTGAATCGTGGATGAGGTTCTGGAACTACTAATGCAAAGGATGGATCAGATGACGCTTATTTACTCTG
GCAATGGACTCAATGGCAAC

Product: putative 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDVFATRRFNFSASHRYWREEWSLEQNEAVFGKCTNRYGHGHNYELFVTVAGAVDPITGMVMNMVELKRLVTMVLDQFDH
KHLNEDTPYFREVIPTTENLVRVLWGLIEPQLPKGVRLAKLRLYENSDLYAEYFGRQQQATFNRRYEFSAAHRLHAQSLT
DEANREIYGKCNNPNGHGHNYQLEVTVDGLIDAQTGMVIDLVDMDRRVQSVLDNWDHRHLDYQVAEFAEQPSTAENIVVV
LWQRLEALFGSQLKHLRLWETANNVFDYPLSQH

Sequences:

>Translated_273_residues
MDVFATRRFNFSASHRYWREEWSLEQNEAVFGKCTNRYGHGHNYELFVTVAGAVDPITGMVMNMVELKRLVTMVLDQFDH
KHLNEDTPYFREVIPTTENLVRVLWGLIEPQLPKGVRLAKLRLYENSDLYAEYFGRQQQATFNRRYEFSAAHRLHAQSLT
DEANREIYGKCNNPNGHGHNYQLEVTVDGLIDAQTGMVIDLVDMDRRVQSVLDNWDHRHLDYQVAEFAEQPSTAENIVVV
LWQRLEALFGSQLKHLRLWETANNVFDYPLSQH
>Mature_273_residues
MDVFATRRFNFSASHRYWREEWSLEQNEAVFGKCTNRYGHGHNYELFVTVAGAVDPITGMVMNMVELKRLVTMVLDQFDH
KHLNEDTPYFREVIPTTENLVRVLWGLIEPQLPKGVRLAKLRLYENSDLYAEYFGRQQQATFNRRYEFSAAHRLHAQSLT
DEANREIYGKCNNPNGHGHNYQLEVTVDGLIDAQTGMVIDLVDMDRRVQSVLDNWDHRHLDYQVAEFAEQPSTAENIVVV
LWQRLEALFGSQLKHLRLWETANNVFDYPLSQH

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=142, Percent_Identity=43.6619718309859, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532224, Length=135, Percent_Identity=42.2222222222222, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115532226, Length=131, Percent_Identity=42.7480916030534, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19550074, Length=138, Percent_Identity=42.0289855072464, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585378, Length=138, Percent_Identity=42.0289855072464, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 31945; Mature: 31945

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00987 PTPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFATRRFNFSASHRYWREEWSLEQNEAVFGKCTNRYGHGHNYELFVTVAGAVDPITGM
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
VMNMVELKRLVTMVLDQFDHKHLNEDTPYFREVIPTTENLVRVLWGLIEPQLPKGVRLAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEE
LRLYENSDLYAEYFGRQQQATFNRRYEFSAAHRLHAQSLTDEANREIYGKCNNPNGHGHN
EEEECCCCHHHHHHCCHHHHHHHHCEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
YQLEVTVDGLIDAQTGMVIDLVDMDRRVQSVLDNWDHRHLDYQVAEFAEQPSTAENIVVV
EEEEEEECCEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHH
LWQRLEALFGSQLKHLRLWETANNVFDYPLSQH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDVFATRRFNFSASHRYWREEWSLEQNEAVFGKCTNRYGHGHNYELFVTVAGAVDPITGM
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
VMNMVELKRLVTMVLDQFDHKHLNEDTPYFREVIPTTENLVRVLWGLIEPQLPKGVRLAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEE
LRLYENSDLYAEYFGRQQQATFNRRYEFSAAHRLHAQSLTDEANREIYGKCNNPNGHGHN
EEEECCCCHHHHHHCCHHHHHHHHCEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
YQLEVTVDGLIDAQTGMVIDLVDMDRRVQSVLDNWDHRHLDYQVAEFAEQPSTAENIVVV
EEEEEEECCEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHH
LWQRLEALFGSQLKHLRLWETANNVFDYPLSQH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA