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The map label for this gene is gatB

Identifier: 159896630

GI number: 159896630

Start: 125245

End: 126696

Strand: Reverse

Name: gatB

Synonym: Haur_0097

Alternate gene names: 159896630

Gene position: 126696-125245 (Counterclockwise)

Preceding gene: 159896631

Following gene: 159896627

Centisome position: 2.0

GC content: 52.13

Gene sequence:

>1452_bases
ATGAAATATACTGCCACGATTGGGCTAGAAGTGCATGCCCAAATTCTTACCAAATCGAAAATGTTCAGCGGTTGTAACGC
GGCCTATGCCAGCGCTCCCGCCAATAGCTGTATCGATGAAGTCAGCATTGGCTTACCTGGCACCTTGCCAGTTGTCAATC
AGGAAGCAATTCGCAAGGCGGCGCTGCTTGGTTTGGCGCTCAATTGCGAAATTCCTGAGTATTGCGAGTTTTCACGTAAA
TCGTATACCTACCCCGATTTGCCCAAAGCATGGCAAATTACCATGTACGATAAGCCAATTTGTATCAATGGTGAGCTAGA
AATTACCCTTGGCAATGGCGAAACCAAGCGAGTTGGCATCACCCGTGCCCACCTCGAAGAAGATACGGGCATGTTGCAAC
ATGGCGATGAAACCCACTCGCTGGTCGATTACAATCGCTCTGGCGTGCCGTTGCTCGAAATCGTCAGCGAGCCTGATATG
ACCACGCCCGAAGAAGCCCGCTTGTATGCCACCAAATTGCGCCAAATTTTGGTCTTTTTGGGGGTCAACAGCGGCAACCT
CGAAGAAGGCGCGTTGCGGGTTGATGCCAATGTTTCAATTCGCCCTGAAGGCCAAAAAGAATTTGGCACCAAGGTCGAGA
TCAAAAATATGAACTCGTTCCGCAATCTTGAGCGGGCTTTGGTCTATGAACTGGAACGCCAAGAAAAAGTTTTGCGCGAA
GGTGGCACGATTATTCAAGAAACTCGTGGTTGGGACGATGCCGCAGGGATCACCTTGAGCCAACGCTCCAAGGAACATGC
CCACGATTATCGCTACTTCCCTGAGCCAGATTTGCCGCCGCTGGAGTTGAGCCGCGAGTGGGTTGCTGAGCGCCGCGCCG
AGTTGCCTGAATTGCCCGATGCCAAATTTGCTCGATATCTTAGCGAATTTGGCTTATCCAAGCAGGATGCCGCCTTGTTG
AGCGGCGAACGTGAAACCGCCGACTATTTTGAAACCATCGTGGCCACCGCTGGCGCAGCCAATGCAAAGCCCGTTGCCAA
CTGGATTACCGGCGAGTTATTCCGTTTGATCAAAGATGGCGCAGAAACCCTCGCCGAGGTCGCCAAACGGGTTACGCCTG
CCAACCTCACCAGTTTGATCGACGTGGTTGCCAAAGGCGAAATCGGCAGCACTGTCGCCAAGCAAGTCTTTGAAGAAATG
TATCGGACTGGCGAAACGCCAACTGCGATTATCAACGCCAAAGGCTTGCGCCAAATTAGCGATAGCAGCGTTTTGACCCA
AGCTGCCCGCGATGCGATCGCCGCTAATCCCAAAGTGGTCGCCGACTACAAGAGTGGCAAATTGCCAGCGATCAAGTTCT
TGGTTGGTCAGGTGATGCGGGCAACCAAAGGTCAAGCCAATCCACAAGTCGTCGAAGAAGCGCTCAAAACCGAGCTTGAT
GCCACCAATTAA

Upstream 100 bases:

>100_bases
TATACAGCGATGCATCTGCTGGCAACGCTATAACAGCTTGCATAATCAGCCATGATCGGGCACAATCAGCGGGAGATTGT
GCTGTTGCCTCGGAGTTGCT

Downstream 100 bases:

>100_bases
TCGGATTAAATCGAAAAGGCGGCGCAACGCTTGAACGTGTTGCGCCGCCTTTGGTTTAATTAGCGATATTCGACCCACAC
CACACAGCTAAACCCCGGCA

Product: glutamyl-tRNA(Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKYTATIGLEVHAQILTKSKMFSGCNAAYASAPANSCIDEVSIGLPGTLPVVNQEAIRKAALLGLALNCEIPEYCEFSRK
SYTYPDLPKAWQITMYDKPICINGELEITLGNGETKRVGITRAHLEEDTGMLQHGDETHSLVDYNRSGVPLLEIVSEPDM
TTPEEARLYATKLRQILVFLGVNSGNLEEGALRVDANVSIRPEGQKEFGTKVEIKNMNSFRNLERALVYELERQEKVLRE
GGTIIQETRGWDDAAGITLSQRSKEHAHDYRYFPEPDLPPLELSREWVAERRAELPELPDAKFARYLSEFGLSKQDAALL
SGERETADYFETIVATAGAANAKPVANWITGELFRLIKDGAETLAEVAKRVTPANLTSLIDVVAKGEIGSTVAKQVFEEM
YRTGETPTAIINAKGLRQISDSSVLTQAARDAIAANPKVVADYKSGKLPAIKFLVGQVMRATKGQANPQVVEEALKTELD
ATN

Sequences:

>Translated_483_residues
MKYTATIGLEVHAQILTKSKMFSGCNAAYASAPANSCIDEVSIGLPGTLPVVNQEAIRKAALLGLALNCEIPEYCEFSRK
SYTYPDLPKAWQITMYDKPICINGELEITLGNGETKRVGITRAHLEEDTGMLQHGDETHSLVDYNRSGVPLLEIVSEPDM
TTPEEARLYATKLRQILVFLGVNSGNLEEGALRVDANVSIRPEGQKEFGTKVEIKNMNSFRNLERALVYELERQEKVLRE
GGTIIQETRGWDDAAGITLSQRSKEHAHDYRYFPEPDLPPLELSREWVAERRAELPELPDAKFARYLSEFGLSKQDAALL
SGERETADYFETIVATAGAANAKPVANWITGELFRLIKDGAETLAEVAKRVTPANLTSLIDVVAKGEIGSTVAKQVFEEM
YRTGETPTAIINAKGLRQISDSSVLTQAARDAIAANPKVVADYKSGKLPAIKFLVGQVMRATKGQANPQVVEEALKTELD
ATN
>Mature_483_residues
MKYTATIGLEVHAQILTKSKMFSGCNAAYASAPANSCIDEVSIGLPGTLPVVNQEAIRKAALLGLALNCEIPEYCEFSRK
SYTYPDLPKAWQITMYDKPICINGELEITLGNGETKRVGITRAHLEEDTGMLQHGDETHSLVDYNRSGVPLLEIVSEPDM
TTPEEARLYATKLRQILVFLGVNSGNLEEGALRVDANVSIRPEGQKEFGTKVEIKNMNSFRNLERALVYELERQEKVLRE
GGTIIQETRGWDDAAGITLSQRSKEHAHDYRYFPEPDLPPLELSREWVAERRAELPELPDAKFARYLSEFGLSKQDAALL
SGERETADYFETIVATAGAANAKPVANWITGELFRLIKDGAETLAEVAKRVTPANLTSLIDVVAKGEIGSTVAKQVFEEM
YRTGETPTAIINAKGLRQISDSSVLTQAARDAIAANPKVVADYKSGKLPAIKFLVGQVMRATKGQANPQVVEEALKTELD
ATN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=38.0761523046092, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI115534094, Length=343, Percent_Identity=35.2769679300292, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.1663479923518, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=33.1325301204819, Blast_Score=258, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_HERA2 (A9B5C4)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001542877.1
- ProteinModelPortal:   A9B5C4
- SMR:   A9B5C4
- GeneID:   5731990
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0097
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   CLSK2516912
- BioCyc:   HAUR316274:HAUR_0097-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53075; Mature: 53075

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTATIGLEVHAQILTKSKMFSGCNAAYASAPANSCIDEVSIGLPGTLPVVNQEAIRKA
CCEEEEECHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
ALLGLALNCEIPEYCEFSRKSYTYPDLPKAWQITMYDKPICINGELEITLGNGETKRVGI
HHHHEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCEEECCEEEEEECCCCCEEEEE
TRAHLEEDTGMLQHGDETHSLVDYNRSGVPLLEIVSEPDMTTPEEARLYATKLRQILVFL
EHHHHHHCCCHHHCCCCHHHHHHCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHE
GVNSGNLEEGALRVDANVSIRPEGQKEFGTKVEIKNMNSFRNLERALVYELERQEKVLRE
ECCCCCCCCCCEEECCCEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
GGTIIQETRGWDDAAGITLSQRSKEHAHDYRYFPEPDLPPLELSREWVAERRAELPELPD
CCCEEECCCCCCCCCCCEEHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
AKFARYLSEFGLSKQDAALLSGERETADYFETIVATAGAANAKPVANWITGELFRLIKDG
HHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AETLAEVAKRVTPANLTSLIDVVAKGEIGSTVAKQVFEEMYRTGETPTAIINAKGLRQIS
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHC
DSSVLTQAARDAIAANPKVVADYKSGKLPAIKFLVGQVMRATKGQANPQVVEEALKTELD
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
ATN
CCC
>Mature Secondary Structure
MKYTATIGLEVHAQILTKSKMFSGCNAAYASAPANSCIDEVSIGLPGTLPVVNQEAIRKA
CCEEEEECHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
ALLGLALNCEIPEYCEFSRKSYTYPDLPKAWQITMYDKPICINGELEITLGNGETKRVGI
HHHHEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCEEECCEEEEEECCCCCEEEEE
TRAHLEEDTGMLQHGDETHSLVDYNRSGVPLLEIVSEPDMTTPEEARLYATKLRQILVFL
EHHHHHHCCCHHHCCCCHHHHHHCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHE
GVNSGNLEEGALRVDANVSIRPEGQKEFGTKVEIKNMNSFRNLERALVYELERQEKVLRE
ECCCCCCCCCCEEECCCEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHC
GGTIIQETRGWDDAAGITLSQRSKEHAHDYRYFPEPDLPPLELSREWVAERRAELPELPD
CCCEEECCCCCCCCCCCEEHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
AKFARYLSEFGLSKQDAALLSGERETADYFETIVATAGAANAKPVANWITGELFRLIKDG
HHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AETLAEVAKRVTPANLTSLIDVVAKGEIGSTVAKQVFEEMYRTGETPTAIINAKGLRQIS
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHC
DSSVLTQAARDAIAANPKVVADYKSGKLPAIKFLVGQVMRATKGQANPQVVEEALKTELD
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
ATN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA