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The map label for this gene is vfr [H]

Identifier: 159896582

GI number: 159896582

Start: 64882

End: 65640

Strand: Direct

Name: vfr [H]

Synonym: Haur_0049

Alternate gene names: 159896582

Gene position: 64882-65640 (Clockwise)

Preceding gene: 159896581

Following gene: 159896583

Centisome position: 1.02

GC content: 51.78

Gene sequence:

>759_bases
ATGAATCCAACCATCACAACGATTACTCCGAGTTTTGGCTGGCGCATGCTAGGAGATAGCCCAATGTTGAGCGATACAGA
ACTGGCGTTACTCCGCGATGTTGAGCTATTTGACGGCCTTGCTCCTGAGCAGGCCGCTCAAATTAGTCGGCGCTTGGTGC
GGCGTACCTTCCCTGCTGGAACCAATTTGATGTCGGTGGAGCAACCAGGCGAGGTTGTGTATATTATTCTGAATGGCACG
GTCAAAATTCATGTTGAGCAAGCTGATGGCACCGATGTGATCATCGCGATGCTCGGGGCTGGCGATACCGTCGGTGAAAT
GAGCTTAATCGATAGCGCTGGCCGCTCGGCAACCGTGGTCACTCAAGAAGAGTCAACCTTGCTTTGGATGAATCGCACAG
CTTTTTTGGAAGCATTGCAAAACGCTCCGGTGATTACCTTAAACCTTGTGCGCATGCTTTCGAGCCGTTTACGGCTTGCC
AATGAGCAAATTCAGGCTTTAGCGACGCTCGATGTGCATGGACGGGTGGCTCGCCAGCTCTTAGCCTTTGCCCAAAAATA
CGGCCACGCCGATGAAACGGGCACGGTGCAAATTCCGATTCGGCTGACCCAAAGCGATTTAGCAGGCTTGGTCGGAGCAT
CACGCGAACGAGTCAACCAAGTGATCGGCTATTTCAAACAACGCCGCTATCTGAGTGTTGATCAACATTATCACATCAGC
TTGCACAACCTTGAAGCCTTGGCCAAACGGATTAGGTAG

Upstream 100 bases:

>100_bases
CTGAAGTAGCCAGCGTCGCGGATGCCAGCACTGCCGTAACCGAGCCATTGAGCAGCGACGTTGCAAGTTGATCAACGGTA
GCAGGTGGAGGAACACGCCC

Downstream 100 bases:

>100_bases
ATCGTGCAGGCAAAATAATCTTGAAAAGGGTATTGGAATTCAACCAATGCTCTTTTTATTTAACTGCTAGTTCTAATGAT
TGGAGAGAATTATGCTCTAC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNPTITTITPSFGWRMLGDSPMLSDTELALLRDVELFDGLAPEQAAQISRRLVRRTFPAGTNLMSVEQPGEVVYIILNGT
VKIHVEQADGTDVIIAMLGAGDTVGEMSLIDSAGRSATVVTQEESTLLWMNRTAFLEALQNAPVITLNLVRMLSSRLRLA
NEQIQALATLDVHGRVARQLLAFAQKYGHADETGTVQIPIRLTQSDLAGLVGASRERVNQVIGYFKQRRYLSVDQHYHIS
LHNLEALAKRIR

Sequences:

>Translated_252_residues
MNPTITTITPSFGWRMLGDSPMLSDTELALLRDVELFDGLAPEQAAQISRRLVRRTFPAGTNLMSVEQPGEVVYIILNGT
VKIHVEQADGTDVIIAMLGAGDTVGEMSLIDSAGRSATVVTQEESTLLWMNRTAFLEALQNAPVITLNLVRMLSSRLRLA
NEQIQALATLDVHGRVARQLLAFAQKYGHADETGTVQIPIRLTQSDLAGLVGASRERVNQVIGYFKQRRYLSVDQHYHIS
LHNLEALAKRIR
>Mature_252_residues
MNPTITTITPSFGWRMLGDSPMLSDTELALLRDVELFDGLAPEQAAQISRRLVRRTFPAGTNLMSVEQPGEVVYIILNGT
VKIHVEQADGTDVIIAMLGAGDTVGEMSLIDSAGRSATVVTQEESTLLWMNRTAFLEALQNAPVITLNLVRMLSSRLRLA
NEQIQALATLDVHGRVARQLLAFAQKYGHADETGTVQIPIRLTQSDLAGLVGASRERVNQVIGYFKQRRYLSVDQHYHIS
LHNLEALAKRIR

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=177, Percent_Identity=27.1186440677966, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27830; Mature: 27830

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTITTITPSFGWRMLGDSPMLSDTELALLRDVELFDGLAPEQAAQISRRLVRRTFPAG
CCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
TNLMSVEQPGEVVYIILNGTVKIHVEQADGTDVIIAMLGAGDTVGEMSLIDSAGRSATVV
CCEEEECCCCCEEEEEEECEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEE
TQEESTLLWMNRTAFLEALQNAPVITLNLVRMLSSRLRLANEQIQALATLDVHGRVARQL
EECCCEEEEECHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAQKYGHADETGTVQIPIRLTQSDLAGLVGASRERVNQVIGYFKQRRYLSVDQHYHIS
HHHHHHHCCCCCCCEEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCEEEEE
LHNLEALAKRIR
HHHHHHHHHHCC
>Mature Secondary Structure
MNPTITTITPSFGWRMLGDSPMLSDTELALLRDVELFDGLAPEQAAQISRRLVRRTFPAG
CCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
TNLMSVEQPGEVVYIILNGTVKIHVEQADGTDVIIAMLGAGDTVGEMSLIDSAGRSATVV
CCEEEECCCCCEEEEEEECEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEE
TQEESTLLWMNRTAFLEALQNAPVITLNLVRMLSSRLRLANEQIQALATLDVHGRVARQL
EECCCEEEEECHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAQKYGHADETGTVQIPIRLTQSDLAGLVGASRERVNQVIGYFKQRRYLSVDQHYHIS
HHHHHHHCCCCCCCEEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCEEEEE
LHNLEALAKRIR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]