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The map label for this gene is rutB [H]

Identifier: 15928231

GI number: 15928231

Start: 2741719

End: 2742279

Strand: Reverse

Name: rutB [H]

Synonym: SA2438

Alternate gene names: 15928231

Gene position: 2742279-2741719 (Counterclockwise)

Preceding gene: 15928232

Following gene: 15928229

Centisome position: 97.42

GC content: 34.94

Gene sequence:

>561_bases
ATGTCTCGAAAAACGGCGCTATTAGTTTTGGATATGCAAGAAGGTATAGCGAGTAGTGTACCTAGAATAAAAAATATTAT
TAAAGCGAATCAGAGAGCAATTGAAGCAGCAAGACAACATCGAATACCAGTCATTTTCATACGTTTAGTGTTAGATAAGC
ATTTTAATGATGTCTCCTCGAGTAATAAAGTGTTTTCAACAATTAAAGCTCAAGGATATGCGATTACTGAAGCAGATGCA
TCTACACGAATACTTGAAGATTTAGCACCACTAGAAGATGAGCCGATTATTTCTAAGCGACGCTTTAGCGCATTTACAGG
TAGTTACTTGGAAGTTTATTTACGTGCAAATGATATTAATCATTTAGTATTAACGGGTGTCTCTACGAGTGGAGCTGTAT
TGAGCACGGCATTAGAAAGTGTAGATAAAGACTATTATATTACTGTTTTAGAAGATGCTGTTGGTGATAGATCAGATGAT
AAACATGACTTTATTATTGAACAAATTTTATCACGCTCATGTGACATTGAATCCGTAGAGTCATGGAAAAGTAGTTTATA
G

Upstream 100 bases:

>100_bases
ATTGTGAAGTATATGCAAAATGCAATGAATAGATTGTCCATCATTTTAACGTTATAATGAATTTAACGACTTAGAACTAC
AGAAGTAAAGGAGAATGAAG

Downstream 100 bases:

>100_bases
TTAATATAACGTCAATTAAAGCTAGGCAGTAATGTTTGAAAATAAGTACATTTGCTCATATTTATAAAATGTGTGAGATG
ACAATTGAAACGGATATGAT

Product: hypothetical protein

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADA
STRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
KHDFIIEQILSRSCDIESVESWKSSL

Sequences:

>Translated_186_residues
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADA
STRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
KHDFIIEQILSRSCDIESVESWKSSL
>Mature_185_residues
SRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADAS
TRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDK
HDFIIEQILSRSCDIESVESWKSSL

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081820, Length=202, Percent_Identity=25.2475247524752, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20730; Mature: 20599

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSS
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDIN
CCHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCC
HLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDKHDFIIEQILSRSCDIESVE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHCCCCHHHHH
SWKSSL
HHHHCC
>Mature Secondary Structure 
SRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSS
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDIN
CCHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCC
HLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDKHDFIIEQILSRSCDIESVE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHCCCCHHHHH
SWKSSL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA