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The map label for this gene is narH

Identifier: 15927974

GI number: 15927974

Start: 2451164

End: 2452723

Strand: Reverse

Name: narH

Synonym: SA2184

Alternate gene names: 15927974

Gene position: 2452723-2451164 (Counterclockwise)

Preceding gene: 15927975

Following gene: 15927973

Centisome position: 87.14

GC content: 37.82

Gene sequence:

>1560_bases
TTGAAGATTAAAGCGCAAGTTGCGATGGTATTAAATTTAGATAAATGCATAGGATGCCATACGTGTAGTGTGACATGTAA
AAACACTTGGACAAATCGTCCAGGTGCTGAGTACATGTGGTTCAATAACGTAGAAACGAAGCCAGGTGTAGGGTATCCGA
AACGTTGGGAAGACCAAGAACACTACAAAGGTGGTTGGGTACTAAATCGTAAAGGGAAACTTGAATTAAAATCTGGAAGT
AGAATTTCAAAAATTGCTTTAGGTAAAATTTTTTATAACCCAGATATGCCATTAATAAAAGATTATTATGAACCATGGAA
CTATAATTATGAACATTTAACAACTGCGAAATCAGGGAAGCATTCGCCAGTTGCTAGAGCGTATTCAGAAATTACAGGGG
ATAACATTGAAATTGAATGGGGACCTAACTGGGAAGATGACTTAGCAGGTGGTCATGTTACAGGCCCAAAAGATCCTAAC
ATCCAAAAAATAGAAGAAGATATTAAATTCCAATTTGACGAAACTTTTATGATGTATTTACCACGTTTATGTGAACATTG
CTTGAATCCAAGTTGCGTTGCATCGTGCCCTTCAGGTGCTATGTATAAACGTGATGAAGATGGCATTGTATTAGTTGATC
AAGACGCATGTCGTGGATGGCGTTACTGTATGACAGGTTGTCCATATAAGAAAGTTTACTTCAATTGGAAAACGAACAAA
GCTGAAAAATGTACTTTCTGTTTTCCGAGAATCGAGGCGGGTATGCCTACGGTATGCTCTGAAACTTGTACAGGACGTAT
GCGTTACTTAGGTGTTTTATTATATGATGCAGATCGAGTTCATGAAGCGGCTTCAGCAGTAGACGAGAAAGGTTTATACG
AAAAACAATTAGATATATTCTTAAATCCATTTGACGAAGAAGTCATTGCTCAAGCTGAAAAAGATGGTATTGGTTATGAT
TGGATTGAAGCTGCACAAAACTCTCCAATTTATAAGTTAGCAATTGAATATAAACTTGCATTTCCGTTACATCCTGAATT
TAGAACGATGCCGATGGTTTGGTATTGCCCACCACTTAGCCCAATTATGAGCTATTTTGAAGGTAAAAATACAACTCAAA
ATCCAGATGCGATTTTCCCAGCCATTGAAGAAATGCGTTTACCTATTGAATACTTAGCTAATATTTTCACTGCAGGAGAT
ACAGAGCCAGTAAAAGGAGCCCTACAACGTATGGCAATGATGAGAAGTTATATGAGATCTCAAGTTACTCAACAGCCATT
TGACACGTCTCGATTAGAGCGATTAGGTATAACCGAGCGCCAAACTAAAGACATGTATCGTTTATTAGGACTAGCTAAAT
ATGAAGATCGATTTGTTATTCCAACATCACACAAAGAAACCTATTTAGATACGTATCACGCACAAGGTAGTACAGGATAC
AATTACGGCGGCGAGCATTTTGGAGATAACTGTGAAGGCTGTGGCGTTGCAGTAGGTTCAGGGAAAACTGGTCAAGAAAT
TTATAATGAGAATTTCTATGGAGGGATTTTCCGTGATTAA

Upstream 100 bases:

>100_bases
GGATACGCACAAATTAGTTATCACTTTAATTATTATGGACCAATTGGGAACCAAAGGGATTTATATGTAGCAGTTAGAAA
GATGAAGGAGGTTAATTGGC

Downstream 100 bases:

>100_bases
TTTCGATAATTTTAAAAAATATCAAGAAAGTTTTGGATATATGGCACAACAGTTATGTTTTCCAGAAAAATTAACTTTTC
ATCCAAAAACTTTTGAAGAA

Product: nitrate reductase beta chain narH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

Sequences:

>Translated_519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
>Mature_519_residues
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGS
RISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYD
WIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGD
TEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=495, Percent_Identity=60, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=494, Percent_Identity=58.502024291498, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1787872, Length=109, Percent_Identity=40.3669724770642, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787122, Length=109, Percent_Identity=40.3669724770642, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=40.2173913043478, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790326, Length=116, Percent_Identity=31.8965517241379, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59373; Mature: 59373

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQE
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
HYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGK
HCCCCEEECCCCCEEECCCCCEEEEEEEEEEECCCCCHHHHHCCCCCCCHHEEEECCCCC
HSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYL
CCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHEEECHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIF
CCEEEEECCHHCCCCCHHHHHHHCCHHHHEEEEEECHHHHHHHHHHHHHCCCHHHHHHHE
LNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLS
ECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
HHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCC
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
CCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQE
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
HYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGK
HCCCCEEECCCCCEEECCCCCEEEEEEEEEEECCCCCHHHHHCCCCCCCHHEEEECCCCC
HSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYL
CCHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHEEECHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIF
CCEEEEECCHHCCCCCHHHHHHHCCHHHHEEEEEECHHHHHHHHHHHHHCCCHHHHHHHE
LNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLS
ECCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRS
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGY
HHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCC
NYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD
CCCCHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]