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The map label for this gene is pbp2

Identifier: 15927031

GI number: 15927031

Start: 1486656

End: 1488839

Strand: Direct

Name: pbp2

Synonym: SA1283

Alternate gene names: 15927031

Gene position: 1486656-1488839 (Clockwise)

Preceding gene: 15927030

Following gene: 15927060

Centisome position: 52.82

GC content: 35.58

Gene sequence:

>2184_bases
ATGACGGAAAACAAAGGATCTTCTCAGCCTAAGAAAAACGGTAATAATGGTGGGAAATCCAACTCAAAAAAGAATAGAAA
TGTGAAGAGAACGATTATTAAGATTATTGGCTTCATGATTATTGCATTTTTCGTTGTTCTTTTACTAGGTATCTTATTGT
TTGCTTATTATGCTTGGAAAGCACCTGCTTTTACCGAAGCTAAATTACAAGATCCGATTCCTGCAAAGATATATGACAAG
AACGGAGAACTTGTTAAAACATTAGATAATGGCCAAAGACATGAGCATGTAAATTTAAAAGACGTGCCGAAATCAATGAA
AGACGCAGTACTTGCAACTGAAGACAATCGTTTCTACGAACATGGCGCACTTGATTATAAACGTTTATTCGGTGCAATTG
GTAAGAACTTGACTGGTGGATTTGGTTCTGAAGGTGCCTCAACATTAACACAACAAGTTGTTAAAGATGCATTTTTATCA
CAACATAAATCTATTGGACGTAAAGCTCAAGAAGCATACTTATCATATCGTTTAGAACAAGAGTATAGTAAAGATGATAT
CTTCCAAGTATATCTAAACAAAATTTACTGTTCTGATGGCGTAACAGGTATTAAAGCTGCTGCTAAGTATTACTTTAATA
AAGATTTAAAAGATTTAAACTTAGCGGAAGAAGCTTATTTAGCCGGTTTACCTCAGGTTCCAAACAACTATAATATTTAT
GATCATCCAAAAGCTGCTGAAGATCGTAAAAACACTGTTTTATACTTAATGCATTATCATAAACGCATTACAGATAAACA
GTGGGAAGATGCTAAGAAAATCGATTTAAAAGCGAACTTAGTAAATCGTACTCCTGAAGAACGTCAAAACATTGATACAA
ATCAAGATTCTGAGTATAATTCATACGTTAACTTTGTGAAATCTGAATTAATGAATAATAAAGCATTCAAAGATGAAAAT
TTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAAAAAACATTACAAAATGATGT
TGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTTGGTGCAACGATTCTTGATAGTAAAACTGGTGGTTTAGTTG
CTATATCTGGTGGACGTGATTTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTA
AAACCTTTCTTAGCGTATGGACCTGCCATTGAAAATATGAAATGGGCAACAAACCATGCGATTCAAGATGAATCTTCATA
TCAAGTTGACGGTTCTACATTTAGAAACTATGATACGAAGAGTCACGGTACTGTATCTATTTATGATGCTTTACGACAAA
GTTTCAATATCCCAGCTTTAAAAGCATGGCAATCAGTTAAGCAAAATGCTGGTAACGATGCACCTAAGAAATTCGCTGCC
AAACTTGGCTTAAACTACGAAGGCGATATTGGTCCATCTGAAGTACTTGGTGGTTCTGCTTCAGAATTCTCACCAACACA
ATTAGCATCAGCATTTGCTGCAATCGCTAACGGTGGTACTTATAACAACGCGCATTCAATTCAAAAAGTAGTTACTCGTG
ACGGTGAAACAATCGAATACGATCATACTAGCCATAAAGCGATGAGTGATTACACTGCATACATGTTAGCTGAGATGCTA
AAAGGTACATTTAAACCATATGGTTCTGCATATGGCCATGGTGTATCTGGAGTAAATATGGGTGCTAAGACAGGTACTGG
TACTTACGGTGCTGAAACTTATTCACAATATAATTTACCTGATAATGCAGCGAAAGACGTGTGGATTAACGGCTTTACAC
CTCAATACACTATGTCAGTGTGGATGGGCTTCAGTAAAGTTAAACAATATGGTGAAAACTCATTTGTGGGACATAGCCAA
CAAGAATATCCACAGTTCTTATATGAAAATGTGATGTCAAAAATTTCATCTAGAGATGGCGAAGACTTTAAACGTCCTAG
CTCAGTAAGTGGTAGTATCCCATCAATCAATGTTTCTGGTAGTCAAGATAACAACACTACAAATCGTAGTACACACGGTG
GTAGTGACACATCAGCAAACAGCAGTGGTACTGCACAATCAAATAACAATACTAGATCTCAACAATCTAGAAACAGCGGT
GGATTAACAGGTATATTCAACTAA

Upstream 100 bases:

>100_bases
TACGAAAAAATGGTTACCATATTCCTTATCAGTATCAACCAAGATTAGACTATCTAAAAGCAGTTGATAAGTTGATATTA
GATGAAAGTGAGGACCGCGT

Downstream 100 bases:

>100_bases
TCCACTCAACATAAAATCCTCAGTTATACCATATTTATGGTGTAGCTGAGGATTTTTTTAGGTTCTTCATCTTTTATGGT
GGGAAGGTAAAACTTCCTGC

Product: PBP2

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDK
NGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIY
DHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDEN
LGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSL
KPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAA
KLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEML
KGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQ
QEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSG
GLTGIFN

Sequences:

>Translated_727_residues
MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDK
NGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIY
DHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDEN
LGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSL
KPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAA
KLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEML
KGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQ
QEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSG
GLTGIFN
>Mature_726_residues
TENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKN
GELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQ
HKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYD
HPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENL
GNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLK
PFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAK
LGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLK
GTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQ
EYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGG
LTGIFN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=404, Percent_Identity=31.6831683168317, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1786343, Length=523, Percent_Identity=27.7246653919694, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI1788867, Length=674, Percent_Identity=25.0741839762611, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789601, Length=162, Percent_Identity=33.9506172839506, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 80367; Mature: 80236

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYE
CCCCCHHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCCCHHH
HGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQ
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIY
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCCCCCC
DHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYN
CCCCCCCCCCCCEEEEEHHHHHHCCCHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
SYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHCCCCCCCCCCCCCHHC
GATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHA
CCEEEECCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCC
IQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAA
CCCCCCCEECCCHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
KLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEY
HHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
DHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
DNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDG
CCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
EDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSG
CHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
GLTGIFN
CCCCCCC
>Mature Secondary Structure 
TENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYE
CCCCCHHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCCCHHH
HGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQ
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIY
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCCCCCC
DHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYN
CCCCCCCCCCCCEEEEEHHHHHHCCCHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHH
SYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHCCCCCCCCCCCCCHHC
GATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHA
CCEEEECCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCC
IQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAA
CCCCCCCEECCCHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
KLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEY
HHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
DHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
DNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDG
CCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
EDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSG
CHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
GLTGIFN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]