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The map label for this gene is smpB

Identifier: 15926458

GI number: 15926458

Start: 843242

End: 843706

Strand: Direct

Name: smpB

Synonym: SA0736

Alternate gene names: 15926458

Gene position: 843242-843706 (Clockwise)

Preceding gene: 15926457

Following gene: 15926459

Centisome position: 29.96

GC content: 35.48

Gene sequence:

>465_bases
ATGGCTAAGAAGAAATCACCAGGTACATTAGCGGAAAATCGTAAAGCAAGACATGATTATAATATTGAAGATACGATTGA
AGCGGGAATCGTATTACAAGGTACAGAAATAAAATCGATTCGCCGAGGTAGTGCTAACCTTAAAGATAGTTACGCGCAAG
TTAAAAACGGTGAAATGTATTTGAATAATATGCATATAGCACCATACGAAGAAGGGAATCGTTTTAATCACGATCCTCTT
CGTTCTCGAAAATTATTATTGCATAAACGTGAAATCTTTAAATTAGGTGAACAAACTCGAGAAATTGGTTATTCGATTGT
GCCGTTAAAGCTTTATTTGAAGCATGGACATTGTAAAGTATTACTTGGTGTTGCACGAGGTAAGAAAAAATATGATAAAC
GTCAAGCTTTGAAAGAAAAAGCAGTCAAACGAGATGTTGCGCGCGATATGAAAGCCCGTTATTAA

Upstream 100 bases:

>100_bases
TAAATCAGGTAATAGTAAGCATAAGCCATTTTATAAAGATAAAAGTGTGAAAAAGAAAGCACGTCGTAAGAAAAAATAAG
CAGCAATGAGGTGAGTATGA

Downstream 100 bases:

>100_bases
GCGATTTAGTTGCTTAATCGGGCTATATTTGATATAGTTATATGTGCTTTTGTAAATTACAAAAGTATGATTTGTTTGAT
TTATTATTTCGGGGACGTTC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGSANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPL
RSRKLLLHKREIFKLGEQTREIGYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY

Sequences:

>Translated_154_residues
MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGSANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPL
RSRKLLLHKREIFKLGEQTREIGYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY
>Mature_153_residues
AKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGSANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLR
SRKLLLHKREIFKLGEQTREIGYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=156, Percent_Identity=46.7948717948718, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17804; Mature: 17673

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGSANLKDSYAQVKNGEMY
CCCCCCCCCCHHCCCCCCCCCCHHHHCCCEEEECCHHHHHHCCCCCCHHHHHHHCCCEEE
LNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIFKLGEQTREIGYSIVPLKLYLKHGHCKV
EECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEE
LLGVARGKKKYDKRQALKEKAVKRDVARDMKARY
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGSANLKDSYAQVKNGEMY
CCCCCCCCCHHCCCCCCCCCCHHHHCCCEEEECCHHHHHHCCCCCCHHHHHHHCCCEEE
LNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIFKLGEQTREIGYSIVPLKLYLKHGHCKV
EECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEE
LLGVARGKKKYDKRQALKEKAVKRDVARDMKARY
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA