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The map label for this gene is trmE

Identifier: 15925702

GI number: 15925702

Start: 2876168

End: 2877547

Strand: Reverse

Name: trmE

Synonym: SAV2712

Alternate gene names: 15925702

Gene position: 2877547-2876168 (Counterclockwise)

Preceding gene: 15925703

Following gene: 15925701

Centisome position: 99.97

GC content: 34.13

Gene sequence:

>1380_bases
ATGGATTTAGATACAATTACGAGTATTTCAACACCAATGGGTGAAGGGGCAATTGGAATTGTTCGATTGTCTGGACCGCA
AGCCGTTGAAATTGCTGACAAATTATATAAAGGAAAACATCTTTTAAATGATGTTCCATCACATACGATTAATTACGGTC
ATATTATTGATCCAGAGTCTAAAGAAGTGGTTGAAGAAGTTATGGTGTCTGTGTTAAGAGCGCCAAAAACATTTACACGC
GAAGATATTATAGAAATTAATTGTCATGGTGGTATTTTAACGATTAATAGAGTGCTGGAACTTACTATGACATATGGCGC
TAGAATGGCTGAGCCAGGTGAATTTACAAAACGTGCCTTTTTAAATGGTCGTATTGATTTATCTCAAGCTGAAGCAGTTA
TGGACTTTATTCGCTCGAAGACAGATAGAGCTTCTAAAGTTGCGATGAATCAAATTGAAGGTCGTCTAAGTGACTTAATC
AAAAAACAACGTCAATCTATATTAGAGATACTCGCTCAAGTGGAAGTGAATATTGATTATCCTGAATACGATGATGTTGA
AGATGCGACTACTGAATTTCTTTTAGAGCAGTCTAAAGAAATCAAACAGGAAATTAATCGTTTATTAGATACCGGTGCGC
AGGGCAAAATTATGCGTGAAGGTTTATCTACAGTTATTGTTGGTAAACCAAACGTAGGTAAATCATCGATGTTAAATAAT
TTAATACAAGATAATAAAGCGATTGTAACTGAGGTAGCAGGTACTACAAGAGATGTCTTAGAAGAGTACGTCAATGTTCG
TAGCGTGCCATTAAGATTAGTTGATACTGCTGGTATACGCGAGACAGAAGATATAGTAGAGAAGATTGGTGTTGAACGCT
CTAGAAAGGCTCTTAGCCAAGCAGACTTAATTTTATTTGTATTAAACAATAACGAAGCATTGACTCAAGAAGATTACACA
TTATATGAAGTGGTTAAAAATGAAGATGTAATCGTAATTGTTAATAAAATGGATTTAGAGCAAAACATAGATATTAATGA
AGTTAAAGATATGATAGGTGATACGCCATTAATTCAAACTTCAATGTTAAAACAAGAAGGTATTGATGAATTAGAAATAC
AAATTCGAGATTTGTTCTTTGGTGGAGAAGTACAAAATCAAGATATGACTTATGTTTCTAATTCAAGACATATTTCATTA
TTAAAACAAGCAAGACAAACGATACAAGATGCGATTGATGCAGCAGAATCTGGTGTGCCTATGGATATGGTACAAATTGA
TTTAACTAGAACTTGGGAAATATTAGGAGAAATTATTGGTGAGACTGCAAGTGATGAACTCATCGATCAGTTATTCAGTC
AATTCTGCTTAGGTAAATAG

Upstream 100 bases:

>100_bases
CAGACAAACGTAAGACTGACCTTAGGGTTAAGACTGACCTTAGGGTTATAATAACTTACTTTAAAATGTATTCACATCGT
TGAAGGAGGCGAGTAGCAAT

Downstream 100 bases:

>100_bases
TAATTGAAATAGACGGAATATCGTCTTAAGAAGGCTAGTAAGATATCAAATAAGGAGGTTTATATTGTGGTTCAAGAATA
TGATGTAATCGTTATAGGTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRSVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRSVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
>Mature_459_residues
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTR
EDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
LIQDNKAIVTEVAGTTRDVLEEYVNVRSVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYT
LYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISL
LKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=480, Percent_Identity=30.4166666666667, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=28.3464566929134, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI193082993, Length=469, Percent_Identity=29.8507462686567, Blast_Score=188, Evalue=8e-48,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=32.6923076923077, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI87082120, Length=144, Percent_Identity=32.6388888888889, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=29.3361884368308, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=28.7755102040816, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=30.4979253112033, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_STAA1 (A7X7A8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001443286.1
- ProteinModelPortal:   A7X7A8
- SMR:   A7X7A8
- STRING:   A7X7A8
- EnsemblBacteria:   EBSTAT00000003356
- GeneID:   5559303
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2696
- eggNOG:   COG0486
- GeneTree:   EBGT00050000024727
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   SAUR418127:SAHV_2696-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51372; Mature: 51372

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 85-85 BINDING 124-124 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPES
CCCCHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
KEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDY
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LIQDNKAIVTEVAGTTRDVLEEYVNVRSVPLRLVDTAGIRETEDIVEKIGVERSRKALSQ
HHHCCCHHHHHHCCCHHHHHHHHHCHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCC
ADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQT
CCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISLLKQARQTIQDAIDAAESGVP
HHHHHCCCCHHHHEEEHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
MDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
HHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPES
CCCCHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
KEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDY
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
PEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LIQDNKAIVTEVAGTTRDVLEEYVNVRSVPLRLVDTAGIRETEDIVEKIGVERSRKALSQ
HHHCCCHHHHHHCCCHHHHHHHHHCHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHCC
ADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQT
CCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISLLKQARQTIQDAIDAAESGVP
HHHHHCCCCHHHHEEEHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
MDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK
HHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA