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The map label for this gene is cysJ [H]

Identifier: 15925610

GI number: 15925610

Start: 2769683

End: 2771563

Strand: Reverse

Name: cysJ [H]

Synonym: SAV2620

Alternate gene names: 15925610

Gene position: 2771563-2769683 (Counterclockwise)

Preceding gene: 15925611

Following gene: 15925609

Centisome position: 96.28

GC content: 34.88

Gene sequence:

>1881_bases
TTGAAATTAAATACATCTAATAGTCCATTTACAGAAAAACAAGTCACAGAGATTAACAATCTGCTGCAAACATTAACAGA
GAGCCAACAACAGTGGCTTAGTGGTTTTTTACTAGCTAATAGTAACGATACGACAAGTGATAGTAATCAACAACAATTAG
AGACAGAAGTGTGGCAACAATCACAAATATCAGAAGAACAAGCAACTTCAACAACGTATATGTTACAAAATAAAGAGCCA
CATATCGAAGCTAATCAGCGGCATGTTACAGTGCTATATGGTTCTGAATCAGGTAATGCCATGCGTTTAGCTGAAATTTT
TTCAGAACGTTTAAGTGATATCGGACATCAAGTTGTTTTGATGTCAATGGATGAATATGATACGACAAACATCGCACAGT
TAGAAGATTTATTTATTATTACGTCTACTCATGGTGAAGGAGAACCGCCTGATAATGCATGGGATTTCTTTGAATTTTTA
GAAGACGATAACGCACCTAATTTAAATCATGTGAGATATTCAGTACTAGCTTTAGGTGATCAAACATATGAATTTTTCTG
TCAAGCTGGTAAAGATGTAGATGCTTTACTAGAAAATCTAGGCGCTGAGCGTATATGTAAGCGTGTAGATTGTGATATTG
ATTATGAAGAAGACGCAGAAAAGTGGATGGCAGACATCATTAATATGATTGATACTACATCAGAAGGTATTCAAAGTGAA
TCGGTGATAAGTGAATCAATTAAGTCTGCCAAAGAAAAGAAATATTCTAAATTAAATCCATACCAAGCAGAAGTTTTAGC
GAATATCAATTTAAATGGTACCGATTCAAATAAAGAAACACGACATATAGAATTTTTACTTGATGATTTTAGTGAATCAT
ATGAACCAGGAGATTGTATAGTAGCATTACCGCAAAACGACCCTGAATTGGTTGAAAAACTAATATCCATGTTAGGTTGG
GATCCGCAATCTCCGGTGCCAATTAATGATCGTGGTGATACAGTTCCTATTGTTGAAGCACTAACATCACATTTTGAATT
TACTAAATTAACATTGCCATTATTGAAAAATGCAGATATCTATTTTGACAATGAAGAATTATCTGAACGTATTCAAGATG
AGTCATGGGCGCGTGAATATGTTATAAATCGGGACTTTATAGATTTAATAACAGATTTTCCAACTATAGAATTACAACCT
GAGAATATGTATCAAATCCTTAGAAAATTACCACCAAGAGAGTATTCGATTTCTAGTAGTTTTATGGCAACGCCAGATGA
AGTGCATATTACCGTTGGTACGGTTCGTTATCAAGCACATGGACGTGAGAGAAAAGGTGTATGCTCGGTTCATTTTGCTG
AGCGAATTAAACCAGGCGATATAGTACCAATTTATTTGAAGAAAAATCCGAACTTCAAATTTCCGATGAAGCAAGATATA
CCGGTTATTATGATTGGACCAGGTACTGGAATTGCTCCTTTTAGAGCATATTTACAAGAACGTGAAGAACTTGGTATGAC
TGGAAAAACATGGTTGTTCTTTGGTGATCAACACCGTAGTTCTGACTTTTTATATGAAGAAGAAATAGAAGAATGGCTTG
AAAATGGAAACTTAACACGCGTAGATTTAGCATTTTCAAGAGACCAAGAACACAAAGAATATGTACAGCATCGTATAATG
GAAGAAAGTGAACGTTTCAATGAATGGATTGAGCAAGGCGCAGCAATCTATATTTGTGGCGATGAAAAATGTATGGCGAA
AGATGTCCATCAAGCCATTAAAGATGTATTGGTAAAAGAACGTCATATTTCTCAAGAAGAAGCAGAGTTATTATTGCGAC
AAATGAAACAACAACAACGCTATCAACGTGATGTTTATTAG

Upstream 100 bases:

>100_bases
CAAGTTTGTTTTAATTTATACCATACATATTAAAAAATTGACTAAACTATCGATAAAAAAAGAGTCAATCATCTATTTCA
AGTTTATTGAGGTGAAACAT

Downstream 100 bases:

>100_bases
CGATTGGTGTTAAATATTTTAAGGTGTAATGATGTAAAAAGATATAAAGGATGTTGCTCAACATGAATATGCCATTAATG
ATAGATTTAACAAATAAAAA

Product: sulfite reductase flavoprotein

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINMIDTTSEGIQSE
SVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDRGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Sequences:

>Translated_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINMIDTTSEGIQSE
SVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDRGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY
>Mature_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINMIDTTSEGIQSE
SVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDRGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
ENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=612, Percent_Identity=30.5555555555556, Blast_Score=263, Evalue=5e-70,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=28.1407035175879, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI10835173, Length=656, Percent_Identity=27.8963414634146, Blast_Score=210, Evalue=4e-54,
Organism=Homo sapiens, GI221316705, Length=606, Percent_Identity=27.7227722772277, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=27.9731993299832, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI40254422, Length=655, Percent_Identity=28.0916030534351, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=28.6538461538462, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=27.3333333333333, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI169790956, Length=425, Percent_Identity=28.4705882352941, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI169790958, Length=425, Percent_Identity=28.4705882352941, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI50726981, Length=184, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1789123, Length=539, Percent_Identity=39.7031539888683, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1790181, Length=152, Percent_Identity=32.2368421052632, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1789154, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554134, Length=605, Percent_Identity=30.7438016528926, Blast_Score=256, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17566446, Length=572, Percent_Identity=26.5734265734266, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17531441, Length=255, Percent_Identity=26.6666666666667, Blast_Score=96, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321832, Length=651, Percent_Identity=25.9600614439324, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325305, Length=631, Percent_Identity=24.8811410459588, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=27.1794871794872, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17137192, Length=619, Percent_Identity=29.8869143780291, Blast_Score=246, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24582192, Length=521, Percent_Identity=29.5585412667946, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24583543, Length=531, Percent_Identity=29.1902071563089, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706876, Length=531, Percent_Identity=29.1902071563089, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706872, Length=531, Percent_Identity=29.1902071563089, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24660907, Length=601, Percent_Identity=25.6239600665557, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24660903, Length=601, Percent_Identity=25.6239600665557, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24647438, Length=414, Percent_Identity=25.6038647342995, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 72249; Mature: 72249

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVL
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EECCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINMIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHH
SVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDRGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVL
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EECCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINMIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHH
SVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDRGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]