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The map label for this gene is thiI

Identifier: 15924705

GI number: 15924705

Start: 1833560

End: 1834783

Strand: Reverse

Name: thiI

Synonym: SAV1715

Alternate gene names: 15924705

Gene position: 1834783-1833560 (Counterclockwise)

Preceding gene: 15924706

Following gene: 15924704

Centisome position: 63.74

GC content: 32.92

Gene sequence:

>1224_bases
ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGAAAAAAATTTGTAAATCAATT
AAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGGTTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAAC
TTGAAGACCATGCAGATATAAATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
AAAGTAGAAAAAACAATAGAGGCAATGAGTGCAGCGGCAATTAAATTTGCGCAGCAATTTGAAGAAAACAGCACATTTAA
AATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGATACGTATGAATTACAACGTGAATTGGGTGGTACCGTATTGA
AACAAATTGAAAATGTTTCTGTTAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
ATGTATGAAGAAGTTGTTCCGGGTTCAGGCGGATTACCAGTTGGTACTGGTGGTAAGACGTTACTAATGCTTTCAGGCGG
TATAGACTCACCAGTTGCTGGAATGGAAGTGATGAGACGTGGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCAT
TTACAAGTGATCAAGCAAAAGAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACAATGACTTCAACGAGACGTAT
GATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGGGCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAG
CCAGTCAAACACTTCATAGCATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCATTTGAAGATTGTTGTACAAT
TTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAAAAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGA
TGATTAATCGTGCTGTTGAAAATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
CAATTAATAAATGACTTTTTATAA

Upstream 100 bases:

>100_bases
TAAGATTATCATTTGGGGCTACTACAACTAAAGAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAA
ATTAAGGAGTTGCTAAAATA

Downstream 100 bases:

>100_bases
ATAAAATCCTAGAGTAAATTTAAACATAAGGGGATGTTAAACTATGGATTTGAACTTAACGATGATTATAATCATAATTT
TATTTGGTTTTATCGCGGCG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL

Sequences:

>Translated_407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL
>Mature_407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=386, Percent_Identity=30.3108808290155, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_STAA1 (A7X3E5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442291.1
- ProteinModelPortal:   A7X3E5
- SMR:   A7X3E5
- STRING:   A7X3E5
- EnsemblBacteria:   EBSTAT00000003390
- GeneID:   5559759
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1701
- eggNOG:   COG0301
- GeneTree:   EBGT00050000024980
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   SAUR418127:SAHV_1701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 46229; Mature: 46229

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADI
CCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCH
NEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
TYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKT
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEEEEHHHCCCCCCCCCCCCCCE
LLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHS
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
MYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFE
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCCHH
KVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL
HHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADI
CCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCH
NEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
TYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKT
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEEEEHHHCCCCCCCCCCCCCCE
LLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHS
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
MYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFE
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCCHH
KVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL
HHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA