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The map label for this gene is norB

Identifier: 15924426

GI number: 15924426

Start: 1546612

End: 1548003

Strand: Reverse

Name: norB

Synonym: SAV1436

Alternate gene names: 15924426

Gene position: 1548003-1546612 (Counterclockwise)

Preceding gene: 15924427

Following gene: 15924425

Centisome position: 53.78

GC content: 32.97

Gene sequence:

>1392_bases
ATGGAAAAGCCGTCAAGAGAGGCATTTGAAGGCAATAATAAGTTGTTAATAGGAATTGTTTTAAGTGTAATAACGTTTTG
GCTATTTGCACAATCATTGGTTAATGTTGTACCAATACTTGAAGATAGTTTCAATACAGATATTGGAACGGTTAATATCG
CCGTTAGTATAACTGCTTTATTTTCAGGAATGTTTGTTGTAGGAGCAGGTGGTCTTGCTGATAAATATGGCAGAATTAAA
CTCACGAACATTGGTATTATCTTAAATATATTGGGTTCATTATTAATCATTATTTCAAATATTCCTTTATTACTTATTAT
AGGAAGATTAATTCAAGGACTTTCAGCAGCATGTATTATGCCTGCAACTTTGTCTATTATTAAGTCATATTACATTGGGA
AAGATAGACAACGCGCTTTAAGTTATTGGTCAATTGGCTCATGGGGCGGCTCTGGTGTTTGTTCATTTTTTGGAGGTGCA
GTTGCAACACTTTTAGGTTGGCGTTGGATTTTCATCCTATCAATTATAATTTCATTAATTGCGCTGTTTCTTATTAAAGG
CACACCTGAAACTAAATCTAAATCGATTTCTCTAAATAAATTTGACATTAAAGGTCTGGTTCTTTTAGTCATTATGCTCC
TCACTTTAAATATTTTAATTACTAAAGGATCAGAATTAGGTGTAACCTCACTTCTTTTTATCACTTTATTAGCAATTGCA
ATTGGATCTTTTAGTTTATTTATAGTTCTTGAAAAGCGTGCTACAAATCCTTTAATCGATTTTAAATTATTTAAAAATAA
AGCTTATACAGGTGCAACAGCTTCAAACTTTTTGTTAAATGGTGTTGCGGGAACATTAATAGTAGCCAACACATTTGTTC
AAAGAGGTTTAGGATATTCCTCATTGCAAGCAGGAAGTTTATCAATCACTTATTTAGTAATGGTACTAATTATGATTCGT
GTTGGTGAAAAGTTACTTCAAACACTCGGATGCAAGAAACCAATGTTAATTGGAACAGGAGTTCTTATTGTCGGAGAATG
TCTCATTTCATTAACTTTCTTGCCAGAAATATTGTATGTCATTTGTTGTATTATAGGTTATTTATTCTTTGGTTTAGGAC
TAGGAATATATGCTACACCATCAACAGATACAGCAATTGCAAATGCACCGTTAGAAAAAGTAGGAGTTGCTGCAGGTATC
TATAAAATGGCTTCTGCATTAGGTGGAGCATTTGGCGTCGCATTGAGTGGTGCAGTTTATGCAATCGTATCAAATATGAC
AAACATTTATACTGGTGCAATGATTGCATTATGGTTAAACGCAGGTATGGGAATATTATCATTCGTTATCATTTTGTTAC
TTGTGCCTAAACAAAACGACACTCAATTATGA

Upstream 100 bases:

>100_bases
TTACACATTAAAATCAAAAACCATTCAATTATTCTATCGAACAGACAAATTTCTGTTATGGAATTTGTCTGTTTTCAAAA
GTATAGGGAGGCAAATAGAG

Downstream 100 bases:

>100_bases
TAATTGAGAATTAAATTGAAATCATACAAGTCGCTACAATATTAAACAAAAATATAAACCGATTCTTATGTGTCATTATT
TTAAATGAACATAGGGATTG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

Sequences:

>Translated_463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
>Mature_463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=23.8916256157635, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788710, Length=329, Percent_Identity=25.8358662613982, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=21.6279069767442, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081983, Length=354, Percent_Identity=24.0112994350282, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=235, Percent_Identity=25.9574468085106, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORB_STAA1 (A7X2C6)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442014.1
- ProteinModelPortal:   A7X2C6
- STRING:   A7X2C6
- EnsemblBacteria:   EBSTAT00000003905
- GeneID:   5559599
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1424
- eggNOG:   COG0477
- GeneTree:   EBGT00050000023931
- HOGENOM:   HBG309849
- OMA:   THICTRI
- ProtClustDB:   CLSK884398
- BioCyc:   SAUR418127:SAHV_1424-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49274; Mature: 49274

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe87d5fc)-; HASH(0xeaa0680)-; HASH(0xdadd178)-; HASH(0xee8ec5c)-; HASH(0xea89b24)-; HASH(0xee9127c)-; HASH(0xea7bda8)-; HASH(0xee911f8)-; HASH(0xe6a2e50)-; HASH(0xe1681b8)-; HASH(0xee8ec98)-; HASH(0xee91120)-; HASH(0xe308608)-; HASH(0xea09a90)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITAL
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHEEHHHH
FSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIM
HCCHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
PATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNILITKGSELGVTSLLFITLLAIA
HHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYS
HCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEILYV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
ICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVY
HHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITAL
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHEEHHHH
FSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIM
HCCHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
PATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNILITKGSELGVTSLLFITLLAIA
HHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYS
HCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEILYV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
ICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVY
HHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA