Click here to switch to the map view.

The map label for this gene is nicK [H]

Identifier: 15923398

GI number: 15923398

Start: 451634

End: 452818

Strand: Reverse

Name: nicK [H]

Synonym: SAV0408

Alternate gene names: 15923398

Gene position: 452818-451634 (Counterclockwise)

Preceding gene: 15923399

Following gene: 15923397

Centisome position: 15.73

GC content: 38.06

Gene sequence:

>1185_bases
ATGGAAAACGAAAAAATTTGGATTAAGGAATTGAAAGAAAAGCGACTGGCTTATGGCGTTTCTCAAAATAAACTGGCGGT
TGCTTCTCATATTACCAGACCTTACCTATCGGATATTGAAACAGGCAAGGCAGTCCCTACTCAACAGGTGAAAGAAGATT
TACTGAACGCTTTGGAACGCTTCAATCCCGATAACCCATTGGAGATGTTATTCGACTATGTACGGATTCGATTCCCTACC
AATGATGTGGCTCAAATTATCGGTGAGGTTTTACGTTTGAATATGGATTATATGCTACACGAAGACTTTGGCTATTATTC
TTATCCCGAACACTATCGTTTCGGTGATATTGTTGTCCTAGTCTCTCATGATGTAAGTAAAGGTGTTTTATTAGAACTGA
AAGGAAAAGGCTGTCGGCAATTTGAAAACTTTCTATTGGCTCAACATCGGAGCTGGTATGATTTCTTTCAAGATTGCATG
GAACATAAAGGTATTTTCAAACGCTTGGATTTAGCTATCAACGATAAAACAGGGATTTTGAATATTCCAGATTTGGCAAA
GAAATGTAAACAGGAAGAATGCATTTCCGTCTTTCGAAGCTTTAAAAATTATCGCAGTGGTGAATTGGTTCACCGTGATG
AAAAACCATACATGGGAAACACACTTTATATTGGCTCATTAAAAAGTGAAGTCTATTTCTGTATATATGAGAAAGATTAT
GAGCAATACGTGAAAAATGATGTTCCCTTAGAAGACGCAGAAGTAAAGAATCGGTTTGAAATTCGGTTAAAAAATGACCG
AGCCACCCAAGCCATGAAAGATTTATTGGCACATCAACAGGCGGAAAAAACAGCCTTTGAAATTATCAATCGCTATATCC
GCTTTGCAGATAAAGACGACACTAAACGTCGGAGTGATTGGAAAACTAATGAACGTTGGGAATGGTTTATCGGTAAAAAT
CGTGGAGCATTACGCCTAACCACACAGCCAGAGCCTTATTCTTTTGAACGAACATTGAACTGGTTACATCATCAAGTCGC
CCCTACATTAAAAATCGCAAGCATTTTAGATGTTCTCAACGGTACAACCATTATCTCCACCATGATTCAGGAAGCCAAAC
TGACAGAAAAACATGAGAAATTAATTGAACAACAACATCTAGCCATGGAGGATTTGATTACATGA

Upstream 100 bases:

>100_bases
CTCATCAACCAATGTTGATGGTACAAAGGATTGCGGAAAATATGAAAGGTGTAAACTCAAGGGTCATAGTTTACATCTTT
TTTAGATTGGAGGTTTGCGA

Downstream 100 bases:

>100_bases
GCCTTTAACAAAAGAATTAAAGAAGTTAGGGTTTCAGTGTGGCATTGAGTTTCAAGCGTATATACAAAATACAGGGAAAT
ATACGTCTCTCATTATTGAG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKAVPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPT
NDVAQIIGEVLRLNMDYMLHEDFGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYMGNTLYIGSLKSEVYFCIYEKDY
EQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLLAHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKN
RGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDLIT

Sequences:

>Translated_394_residues
MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKAVPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPT
NDVAQIIGEVLRLNMDYMLHEDFGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYMGNTLYIGSLKSEVYFCIYEKDY
EQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLLAHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKN
RGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDLIT
>Mature_394_residues
MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKAVPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPT
NDVAQIIGEVLRLNMDYMLHEDFGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYMGNTLYIGSLKSEVYFCIYEKDY
EQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLLAHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKN
RGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDLIT

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 46464; Mature: 46464

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKAVPTQQVKEDLLNALER
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHEDFGYYSYPEHYRFGDIVVL
CCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHCHHHHEECCCCCCCCCCCCCCCCEEEE
VSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCMEHKGIFKRLDLAINDKTGIL
EECCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCEEECCCCCCE
NIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYMGNTLYIGSLKSEVYFCIYEKDY
ECHHHHHHHCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEECCCEEEEEEECHH
EQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLLAHQQAEKTAFEIINRYIRFADKDD
HHHHCCCCCCCCHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLN
CHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GTTIISTMIQEAKLTEKHEKLIEQQHLAMEDLIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKAVPTQQVKEDLLNALER
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHEDFGYYSYPEHYRFGDIVVL
CCCCCHHHHHHHHHHEECCCHHHHHHHHHHHHHCHHHHEECCCCCCCCCCCCCCCCEEEE
VSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCMEHKGIFKRLDLAINDKTGIL
EECCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCEEECCCCCCE
NIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYMGNTLYIGSLKSEVYFCIYEKDY
ECHHHHHHHCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEECCCEEEEEEECHH
EQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLLAHQQAEKTAFEIINRYIRFADKDD
HHHHCCCCCCCCHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYSFERTLNWLHHQVAPTLKIASILDVLN
CHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GTTIISTMIQEAKLTEKHEKLIEQQHLAMEDLIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]