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The map label for this gene is yrkE [H]

Identifier: 15923076

GI number: 15923076

Start: 91482

End: 92546

Strand: Direct

Name: yrkE [H]

Synonym: SAV0086

Alternate gene names: 15923076

Gene position: 91482-92546 (Clockwise)

Preceding gene: 15923072

Following gene: 15923077

Centisome position: 3.18

GC content: 31.64

Gene sequence:

>1065_bases
ATGAAACAATACGGAGAAAAGTTTATCGATGAATTTAGTAAAGCAGAATTCGAAAAACTAGCCAAGCAAGGGCAATTAAT
TGATGTTAGAACAGAAGAGGAGTATGCATTAGGACATATCAATGGTTCCATACTTCATCCTGTTGATGAGATTGAGTCAT
TCAATAAAGAAAAAAATAAAACCTATTATGTAATCTGTAGAAGTGGTAACAGAAGTGCTAATGCTAGTAAATATTTAGCT
AAACAAGGTTATAACGTTATAAATCTTGATGGTGGTTATAAAGCTTATGAAGAAAACGATAGTTATGATACACAAGAAGA
ATATAAAAGTATAGAAATTAAAGCAGATCGTAAACAATTTAACTATCGTGGTTTTCAATGTCCAGGGCCAATTGTAAAAA
TTAGTCAAGAAATGAAGAATATTGAAGTAGGTGACCAAATTGAAGTCAAAGTCACAGACCCTGGATTCCCTAGTGATATT
AAAAGTTGGGTGAAACAAACAAGGCATACTTTAGTTAAGCTTGATGAAAATAACAATGGAATTAATGCGATTATTCAAAA
AGAAAAAGCAAAAGATTTAGATATAAATTATTCTGCTAAAGGTACTACAATTGTATTATTTAGTGGAGAATTAGACAAAG
CTGTAGCAGCATTGATTATTGCAAATGGCGCTAGAGCTGCTGGAAAAGATGTAACTATCTTCTTTACTTTTTGGGGGCTT
AATGCATTAAAAAAAGTGCAAACAGTTAATGTTAAAAAGCAAGGTATTGCAAAAATGTTTGATTTAATGTTGCCCCAAAA
GAATATACGAATGCCTCTTTCCAAAATGAATATGTTTGGTTTAGGAAATATGATGATGCGCTACGTAATGAAAAAGAAAA
ATGTTGATTCATTACCAACACTTATCAATCAAGCTATTGAGCAAAATATCAAATTAATCGCTTGTACGATGAGTATGGAT
GTCATGGGTATTCAGAAAGAAGAACTTAGAGATGAAGTTGAGTACGGTGGTGTAGGCACTTATATTGGTGCTACTGAAAA
TGCGAATCATAATTTATTTATCTAA

Upstream 100 bases:

>100_bases
TAATAGATAAACACAAATGTGTCAAATACCCCTAGAGGTATTTGACAAGTTCCATTCAACCGTTTAGAATACCCCTACAG
GTATTTTTAGGGAGGTTATT

Downstream 100 bases:

>100_bases
TTAAATCTATTAATAAAAGGAGTTGTTATCATGTTTTTTAAACAGTTTTACGATAATCATTTATCTCAAGCATCATATTT
AGTGGGTTGTCAACGTACAG

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKQYGEKFIDEFSKAEFEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKAYEENDSYDTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGL
NALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMD
VMGIQKEELRDEVEYGGVGTYIGATENANHNLFI

Sequences:

>Translated_354_residues
MKQYGEKFIDEFSKAEFEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKAYEENDSYDTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGL
NALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMD
VMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
>Mature_354_residues
MKQYGEKFIDEFSKAEFEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLA
KQGYNVINLDGGYKAYEENDSYDTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGL
NALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMD
VMGIQKEELRDEVEYGGVGTYIGATENANHNLFI

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG2210

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 40026; Mature: 40026

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYGEKFIDEFSKAEFEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNK
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCEECCHHHHHHCCCCCCC
TYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEENDSYDTQEEYKSIEIKADRKQF
EEEEEECCCCCCCCHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHEEEEEECHHHC
NYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDENNNG
CCCCEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCC
INAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGL
EEEEEECCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHEECCCCCCCCCEEEEEEECCH
NALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPT
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LINQAIEQNIKLIACTMSMDVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEC
>Mature Secondary Structure
MKQYGEKFIDEFSKAEFEKLAKQGQLIDVRTEEEYALGHINGSILHPVDEIESFNKEKNK
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCEECCHHHHHHCCCCCCC
TYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEENDSYDTQEEYKSIEIKADRKQF
EEEEEECCCCCCCCHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHEEEEEECHHHC
NYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDENNNG
CCCCEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCC
INAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAALIIANGARAAGKDVTIFFTFWGL
EEEEEECCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHEECCCCCCCCCEEEEEEECCH
NALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKMNMFGLGNMMMRYVMKKKNVDSLPT
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LINQAIEQNIKLIACTMSMDVMGIQKEELRDEVEYGGVGTYIGATENANHNLFI
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]