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The map label for this gene is tetB [H]

Identifier: 15922939

GI number: 15922939

Start: 2619883

End: 2621325

Strand: Direct

Name: tetB [H]

Synonym: ST2604

Alternate gene names: 15922939

Gene position: 2619883-2621325 (Clockwise)

Preceding gene: 15922936

Following gene: 15922940

Centisome position: 97.22

GC content: 34.44

Gene sequence:

>1443_bases
ATGGAGAGGACTTTTTTAAGATATGTCCCAATTCTTGCGTTTATAACTATAATGACAATGTATGTAGAAATGGTGATTTT
GCCTTCCTTACCAGAAATAGAGAACCAATTTTCAATAACATCATCAGAAGCCTCATGGATATTATCTTCAGAGACATTAG
CAGGAATGGCTCTTGCTCCTTTTCTTGGAAAATTAGCTGATAATTACGGAAGGAAGAAAGTCCTAGTCATTATTCTTATT
GCGTACACGTTTAGCGTATTTCTATCTGCGATCTCACCCAATTATCCTATCCTTATTCTTTCAAGAAGCATACAAGGAAT
AGGTTTAAGCATAAACCCAATAGCTTATACCATATTACGAGAAAGAATACCAGATAGAGAACTGCCAATAGCTCAAGGAA
TCATTGCATCAACTTTTGCAGTAGGTGCTGCAATAGCGATCCCAATTGGGAGTTATATCTCACAGTATTTCTCATGGGAA
TTTGCGTATGAAACAGCAATTCCCTTCTTACTCTTATCAACTGTTATAGCTTATGTAGTATTACCTTCTTCCGAAAGCAA
AATTATAAGCAGGGAGTTAGATACAGTGGGGATTATTCTTCTCTCCTTATCCTTCTTAGTCTTAGGTGTTGGACTAACTG
AAGCACCTACATGGGGCTGGTTATCTTCACAATTTTTTACATCACTTGCAATTTCTTTCCTTCTTTTCTACATCTTCTCA
TTACATATAAGCAAAACTGATAATCCTGTGATAAATCCTCAGGATTTCAAGAATCCTAATATTGCAGTTCCACTCATCTC
ATCATTTATAACAGGTTTCGGTTTATTTCTCACGTTTCAATCCCTAGTATTTATTTTTGAACTTCCTAGTCCGGTTGGTT
ATGGAATGGATATTCTACAGACAGGTGAAACTATAGCACCTATTGCATTAATAATGCTAGTAGCCGGACCATTTTTTGGA
AGTTTAGTAACAAAAGTTGGTTATAAGAAAGTAATATTGCTGTCCTCTTCGATCTCATCTTTCCTTCTTCTTCTCCTTTC
GTATATTATTTCTATTAAAGTTTCAATAATAGAACTCATGGGTTTCTTGGCTTTAATTTTGTTTTTTATTTCTGGAATGA
ATGTGACTAGAGTGACTTTACTTATAGCCTCTTCAGATAGAGAAAGAATGAGTACTTTAACTGGTACTAATACTGCAATG
AGATTAATGGGAAATACTTTAGGTCCAATAGTAGTTGGATCGTTAGAAGATACTTTTAAAACACCGCTGTTTGAAGGTTA
TATAAATAACATTCCGATGTTTGCTTTTATTCCTTCGAGATTAGCTTTTGAATACTCATTTTTAGTATCTATGGGGACAA
CTGTTTTAGTTTCTATATTAGCTACGAGAATAAAAGAAGAGATTCATAATAGAAGAATCAGCGTATCTATCAGAACCTCT
TAA

Upstream 100 bases:

>100_bases
TATTACTGTTTAATTTCATACTTATATTCATAGATAATATTAATACTATTTCTCCAAATCAAGAGTAAAGGTTTAGTAGT
TTAATGTAGATATATCTTTA

Downstream 100 bases:

>100_bases
CAGTTGTTTAAAAAACTTAATTTCTCCTAACGGCATAAAAATTGATTTATAAAATATAAAAAAGAAAGTTTATAATTATT
CTTGAGATAGTAGTATTATG

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MERTFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWILSSETLAGMALAPFLGKLADNYGRKKVLVIILI
AYTFSVFLSAISPNYPILILSRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAVGAAIAIPIGSYISQYFSWE
FAYETAIPFLLLSTVIAYVVLPSSESKIISRELDTVGIILLSLSFLVLGVGLTEAPTWGWLSSQFFTSLAISFLLFYIFS
LHISKTDNPVINPQDFKNPNIAVPLISSFITGFGLFLTFQSLVFIFELPSPVGYGMDILQTGETIAPIALIMLVAGPFFG
SLVTKVGYKKVILLSSSISSFLLLLLSYIISIKVSIIELMGFLALILFFISGMNVTRVTLLIASSDRERMSTLTGTNTAM
RLMGNTLGPIVVGSLEDTFKTPLFEGYINNIPMFAFIPSRLAFEYSFLVSMGTTVLVSILATRIKEEIHNRRISVSIRTS

Sequences:

>Translated_480_residues
MERTFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWILSSETLAGMALAPFLGKLADNYGRKKVLVIILI
AYTFSVFLSAISPNYPILILSRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAVGAAIAIPIGSYISQYFSWE
FAYETAIPFLLLSTVIAYVVLPSSESKIISRELDTVGIILLSLSFLVLGVGLTEAPTWGWLSSQFFTSLAISFLLFYIFS
LHISKTDNPVINPQDFKNPNIAVPLISSFITGFGLFLTFQSLVFIFELPSPVGYGMDILQTGETIAPIALIMLVAGPFFG
SLVTKVGYKKVILLSSSISSFLLLLLSYIISIKVSIIELMGFLALILFFISGMNVTRVTLLIASSDRERMSTLTGTNTAM
RLMGNTLGPIVVGSLEDTFKTPLFEGYINNIPMFAFIPSRLAFEYSFLVSMGTTVLVSILATRIKEEIHNRRISVSIRTS
>Mature_480_residues
MERTFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWILSSETLAGMALAPFLGKLADNYGRKKVLVIILI
AYTFSVFLSAISPNYPILILSRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAVGAAIAIPIGSYISQYFSWE
FAYETAIPFLLLSTVIAYVVLPSSESKIISRELDTVGIILLSLSFLVLGVGLTEAPTWGWLSSQFFTSLAISFLLFYIFS
LHISKTDNPVINPQDFKNPNIAVPLISSFITGFGLFLTFQSLVFIFELPSPVGYGMDILQTGETIAPIALIMLVAGPFFG
SLVTKVGYKKVILLSSSISSFLLLLLSYIISIKVSIIELMGFLALILFFISGMNVTRVTLLIASSDRERMSTLTGTNTAM
RLMGNTLGPIVVGSLEDTFKTPLFEGYINNIPMFAFIPSRLAFEYSFLVSMGTTVLVSILATRIKEEIHNRRISVSIRTS

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=309, Percent_Identity=24.5954692556634, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789042, Length=332, Percent_Identity=24.6987951807229, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788710, Length=333, Percent_Identity=24.6246246246246, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=149, Percent_Identity=26.8456375838926, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52842; Mature: 52842

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00449 ATPASE_A ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWILSSETLAGMALAP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECHHHHHHHHHH
FLGKLADNYGRKKVLVIILIAYTFSVFLSAISPNYPILILSRSIQGIGLSINPIAYTILR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHH
ERIPDRELPIAQGIIASTFAVGAAIAIPIGSYISQYFSWEFAYETAIPFLLLSTVIAYVV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LPSSESKIISRELDTVGIILLSLSFLVLGVGLTEAPTWGWLSSQFFTSLAISFLLFYIFS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHISKTDNPVINPQDFKNPNIAVPLISSFITGFGLFLTFQSLVFIFELPSPVGYGMDILQ
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
TGETIAPIALIMLVAGPFFGSLVTKVGYKKVILLSSSISSFLLLLLSYIISIKVSIIELM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GFLALILFFISGMNVTRVTLLIASSDRERMSTLTGTNTAMRLMGNTLGPIVVGSLEDTFK
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCEEECCCHHHHC
TPLFEGYINNIPMFAFIPSRLAFEYSFLVSMGTTVLVSILATRIKEEIHNRRISVSIRTS
CHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure
MERTFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWILSSETLAGMALAP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECHHHHHHHHHH
FLGKLADNYGRKKVLVIILIAYTFSVFLSAISPNYPILILSRSIQGIGLSINPIAYTILR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHH
ERIPDRELPIAQGIIASTFAVGAAIAIPIGSYISQYFSWEFAYETAIPFLLLSTVIAYVV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LPSSESKIISRELDTVGIILLSLSFLVLGVGLTEAPTWGWLSSQFFTSLAISFLLFYIFS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHISKTDNPVINPQDFKNPNIAVPLISSFITGFGLFLTFQSLVFIFELPSPVGYGMDILQ
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
TGETIAPIALIMLVAGPFFGSLVTKVGYKKVILLSSSISSFLLLLLSYIISIKVSIIELM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GFLALILFFISGMNVTRVTLLIASSDRERMSTLTGTNTAMRLMGNTLGPIVVGSLEDTFK
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCEEECCCHHHHC
TPLFEGYINNIPMFAFIPSRLAFEYSFLVSMGTTVLVSILATRIKEEIHNRRISVSIRTS
CHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377 [H]