Click here to switch to the map view.

The map label for this gene is btuF [H]

Identifier: 15922529

GI number: 15922529

Start: 2210596

End: 2211417

Strand: Direct

Name: btuF [H]

Synonym: ST2201

Alternate gene names: 15922529

Gene position: 2210596-2211417 (Clockwise)

Preceding gene: 15922528

Following gene: 15922531

Centisome position: 82.03

GC content: 32.48

Gene sequence:

>822_bases
GTGAAAATTTATAATGAAGTATTAGACGAGTATGTTGAAGTTCCTAGACCTATAAAGAAAATTGTCAGTCTAGATCCAGC
TACTACTGAAACTTTATTTATGCTAGGTATAGGTGATCGGATTATTGCTACTGACGCCTTCAGTTATAGACCAGCTGAAG
CTAAAAAAATACCAAAGATTGGCAGCTATACTCATGTAAACATAGAATTTTTAGAGAAAAATAAGCCTGATATAATTTTC
TCTACTACTGGTGCTCAAAAGGATTTAACGAAGAAGCTAATAAACATGGGATATATAGTTTATCCTATTGGTGTTGCTAC
GTCTATCTCAAGGATTCTAAATAACATTATTATCATTGCTAACGTTATAGGAAGATTAGAAGAAGGTAGAATACTTTACT
CATCTTTAATTAATGCTTTATCTTCCTTCCTTAGAAAAAGTGAAAAAAGGCCTAAAGTTTATTTGGAGTTTGATCTTGGG
GGACCAATAACTGCAGGTTTTCCTACTCATGTTAGTGATGCGATTTATCATGTAGGAGGAAAGAATATTTTCGATAATGT
TGAAGACGCTTATTTTACTCCTTCTCCAAATGATATTATTTCACGCGAACCAGACGTAATAATTTATGAGCCTAAGCTAT
ATAGAGATTATGAAGTTGATAGGTTTAAAAAGAGCTTAGAGGAAAGAGGTCTTTCTGCACTCTTAAATAAGCCAATTTTA
GTTACAAAAGGTGATTTTTTAGCACATCAGGGACCTAGTTTTATAACTGAAGGTGTTAAATGGTTATTTTTTTCGCTTGA
ACCTTTTTTTGCTAAATATTGA

Upstream 100 bases:

>100_bases
AATCTACATTACATTACTAGTTATCGAAATCTTAGCTAATCAATAATATTATTGACACATGTACAGTAACATTTTTTAAA
ACTCAATTAATTAATTCTTT

Downstream 100 bases:

>100_bases
CGATATTACACCGTAACCTAGTGCTACTATTCCTAGTACTATGAAAAACTCGCTTACTGTTGTTATTATTCCTAGGGTTG
AGTATAAACTACTTACTTGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKIYNEVLDEYVEVPRPIKKIVSLDPATTETLFMLGIGDRIIATDAFSYRPAEAKKIPKIGSYTHVNIEFLEKNKPDIIF
STTGAQKDLTKKLINMGYIVYPIGVATSISRILNNIIIIANVIGRLEEGRILYSSLINALSSFLRKSEKRPKVYLEFDLG
GPITAGFPTHVSDAIYHVGGKNIFDNVEDAYFTPSPNDIISREPDVIIYEPKLYRDYEVDRFKKSLEERGLSALLNKPIL
VTKGDFLAHQGPSFITEGVKWLFFSLEPFFAKY

Sequences:

>Translated_273_residues
MKIYNEVLDEYVEVPRPIKKIVSLDPATTETLFMLGIGDRIIATDAFSYRPAEAKKIPKIGSYTHVNIEFLEKNKPDIIF
STTGAQKDLTKKLINMGYIVYPIGVATSISRILNNIIIIANVIGRLEEGRILYSSLINALSSFLRKSEKRPKVYLEFDLG
GPITAGFPTHVSDAIYHVGGKNIFDNVEDAYFTPSPNDIISREPDVIIYEPKLYRDYEVDRFKKSLEERGLSALLNKPIL
VTKGDFLAHQGPSFITEGVKWLFFSLEPFFAKY
>Mature_273_residues
MKIYNEVLDEYVEVPRPIKKIVSLDPATTETLFMLGIGDRIIATDAFSYRPAEAKKIPKIGSYTHVNIEFLEKNKPDIIF
STTGAQKDLTKKLINMGYIVYPIGVATSISRILNNIIIIANVIGRLEEGRILYSSLINALSSFLRKSEKRPKVYLEFDLG
GPITAGFPTHVSDAIYHVGGKNIFDNVEDAYFTPSPNDIISREPDVIIYEPKLYRDYEVDRFKKSLEERGLSALLNKPIL
VTKGDFLAHQGPSFITEGVKWLFFSLEPFFAKY

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=192, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30878; Mature: 30878

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYNEVLDEYVEVPRPIKKIVSLDPATTETLFMLGIGDRIIATDAFSYRPAEAKKIPKI
CCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHCCCCC
GSYTHVNIEFLEKNKPDIIFSTTGAQKDLTKKLINMGYIVYPIGVATSISRILNNIIIIA
CCEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
NVIGRLEEGRILYSSLINALSSFLRKSEKRPKVYLEFDLGGPITAGFPTHVSDAIYHVGG
HHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCC
KNIFDNVEDAYFTPSPNDIISREPDVIIYEPKLYRDYEVDRFKKSLEERGLSALLNKPIL
CHHHCCCCCCEECCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
VTKGDFLAHQGPSFITEGVKWLFFSLEPFFAKY
EECCCHHHCCCCHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure
MKIYNEVLDEYVEVPRPIKKIVSLDPATTETLFMLGIGDRIIATDAFSYRPAEAKKIPKI
CCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHCCCCC
GSYTHVNIEFLEKNKPDIIFSTTGAQKDLTKKLINMGYIVYPIGVATSISRILNNIIIIA
CCEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
NVIGRLEEGRILYSSLINALSSFLRKSEKRPKVYLEFDLGGPITAGFPTHVSDAIYHVGG
HHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCC
KNIFDNVEDAYFTPSPNDIISREPDVIIYEPKLYRDYEVDRFKKSLEERGLSALLNKPIL
CHHHCCCCCCEECCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
VTKGDFLAHQGPSFITEGVKWLFFSLEPFFAKY
EECCCHHHCCCCHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA