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The map label for this gene is mdh [H]

Identifier: 15922415

GI number: 15922415

Start: 2097788

End: 2098744

Strand: Direct

Name: mdh [H]

Synonym: ST2090

Alternate gene names: 15922415

Gene position: 2097788-2098744 (Clockwise)

Preceding gene: 15922411

Following gene: 15922416

Centisome position: 77.85

GC content: 36.89

Gene sequence:

>957_bases
GTGAAAATCAAAGTTAAAGAGGCTGAAAGCCTAGTCGAGTCAATACTAAAAAAGAGGGGAGTTGAGAATCCGTCAATTAT
TGCAAAGCATTTTGTAGAGGCTGAGCTTAGGGGCCATTATTCTCATGGTCTCCAAAGAGTTATCCCTCTCGTTAAGGGAA
TAGATTTAGGAACAATAAGAAAAACCGTGCAACTAACAGAAATTAAAAGAGAGGATAATGCAGTCCTTTATGATGCAAAT
TATAGCATAGGAATTCTAGTTTGGCACTATCTAACAGAAAGTCCAAAAGAAACGTTAATCGCAGTTAGAAACTCCTCTCA
TATAGGTTTTTTAGGCTACTATACTAGGAGGATAGCAATGAGACTCGGAAAACCTGCAATTATGATAGGAAATGCTGAAC
CTGCAGTAGTAAAGCCTGGAACTGCGAAAAAAATCATATCAACTACTCCTATAAGTATTGCAATACCTTGTGAAAAGATT
GTAGTTTTAGACATGTCTTTATCTCCTATCGCAAGAGGAAAAATTATTGAGGCTAAAAGAAAGGGAGAGAAGATACCTTA
TGGTGTTACAGTAAATAAGGACGGTGAGATAACTACAGATCCAGATGAGGCTTTGCAAGGCGGATTATTGCCTATAGGAG
GTATGAAAGGGTTCTTCCTAATGATTACCTTAGAATTATTAGTATCTTTTCTAACTGGGAGCGCATTAGCTACTGAGGTT
CAAGGTGTTCTTGATACAACTAAGAGCCCAAACAAGGGAGAGTTCATGATTATTTTGCCCAGGCTTCAGAGTGACTGTCA
TGGTTTATCAGTACTAAAACAATTTGTGGAATATCTACCTGGTGAACATAGTGATGAAATGTTAAAGAGGGATGAAATAG
ATATTGATGAGAAGTTATATAATATCTTCGTTAAATTGGAGGCTGAAGAACAATTCAATTGGACTCAAAACGAATAA

Upstream 100 bases:

>100_bases
GAAATATAACATGAATCATATTGATTTACAAAGTTTATTAATTTTGACGAAACTCACATTATTAGTAAGATTGATAATCT
AATATATCCTTTTTTATATC

Downstream 100 bases:

>100_bases
TTTTTAAAATCAAGTTTAGTAATAAAAAGTAATGAAGAGAGTTAAATTTGTTGTAACAATTCTGTTACTAACTCTCTTTT
TAATAGAGTTTTCTACACCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: (R)-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKIKVKEAESLVESILKKRGVENPSIIAKHFVEAELRGHYSHGLQRVIPLVKGIDLGTIRKTVQLTEIKREDNAVLYDAN
YSIGILVWHYLTESPKETLIAVRNSSHIGFLGYYTRRIAMRLGKPAIMIGNAEPAVVKPGTAKKIISTTPISIAIPCEKI
VVLDMSLSPIARGKIIEAKRKGEKIPYGVTVNKDGEITTDPDEALQGGLLPIGGMKGFFLMITLELLVSFLTGSALATEV
QGVLDTTKSPNKGEFMIILPRLQSDCHGLSVLKQFVEYLPGEHSDEMLKRDEIDIDEKLYNIFVKLEAEEQFNWTQNE

Sequences:

>Translated_318_residues
MKIKVKEAESLVESILKKRGVENPSIIAKHFVEAELRGHYSHGLQRVIPLVKGIDLGTIRKTVQLTEIKREDNAVLYDAN
YSIGILVWHYLTESPKETLIAVRNSSHIGFLGYYTRRIAMRLGKPAIMIGNAEPAVVKPGTAKKIISTTPISIAIPCEKI
VVLDMSLSPIARGKIIEAKRKGEKIPYGVTVNKDGEITTDPDEALQGGLLPIGGMKGFFLMITLELLVSFLTGSALATEV
QGVLDTTKSPNKGEFMIILPRLQSDCHGLSVLKQFVEYLPGEHSDEMLKRDEIDIDEKLYNIFVKLEAEEQFNWTQNE
>Mature_318_residues
MKIKVKEAESLVESILKKRGVENPSIIAKHFVEAELRGHYSHGLQRVIPLVKGIDLGTIRKTVQLTEIKREDNAVLYDAN
YSIGILVWHYLTESPKETLIAVRNSSHIGFLGYYTRRIAMRLGKPAIMIGNAEPAVVKPGTAKKIISTTPISIAIPCEKI
VVLDMSLSPIARGKIIEAKRKGEKIPYGVTVNKDGEITTDPDEALQGGLLPIGGMKGFFLMITLELLVSFLTGSALATEV
QGVLDTTKSPNKGEFMIILPRLQSDCHGLSVLKQFVEYLPGEHSDEMLKRDEIDIDEKLYNIFVKLEAEEQFNWTQNE

Specific function: Acts on oxaloacetate, sulfopyruvate but not on pyruvate. Has a higher selectivity for the coenzyme NADH than for NADPH [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786727, Length=274, Percent_Identity=27.3722627737226, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790000, Length=251, Percent_Identity=27.8884462151394, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787020, Length=317, Percent_Identity=24.2902208201893, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17507179, Length=293, Percent_Identity=29.6928327645051, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536633, Length=314, Percent_Identity=26.7515923566879, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24658010, Length=269, Percent_Identity=26.3940520446097, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45553211, Length=231, Percent_Identity=25.974025974026, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24667912, Length=231, Percent_Identity=25.974025974026, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24667908, Length=231, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667904, Length=231, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.272; =1.1.1.37; =1.1.1.82 [H]

Molecular weight: Translated: 35368; Mature: 35368

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVKEAESLVESILKKRGVENPSIIAKHFVEAELRGHYSHGLQRVIPLVKGIDLGTIR
CEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTVQLTEIKREDNAVLYDANYSIGILVWHYLTESPKETLIAVRNSSHIGFLGYYTRRIAM
HHHHHHHHHCCCCEEEEECCCCEEEEEEEHHCCCCCCEEEEEECCCCEEHHHHHHHHHHH
RLGKPAIMIGNAEPAVVKPGTAKKIISTTPISIAIPCEKIVVLDMSLSPIARGKIIEAKR
HCCCCEEEECCCCCCEECCCCCHHHEECCCEEEEECCCEEEEEECCCCCCCCCCEEEHHH
KGEKIPYGVTVNKDGEITTDPDEALQGGLLPIGGMKGFFLMITLELLVSFLTGSALATEV
CCCCCCCEEEECCCCCEECCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHH
QGVLDTTKSPNKGEFMIILPRLQSDCHGLSVLKQFVEYLPGEHSDEMLKRDEIDIDEKLY
HHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHEE
NIFVKLEAEEQFNWTQNE
EEEEEEEEHHCCCCCCCC
>Mature Secondary Structure
MKIKVKEAESLVESILKKRGVENPSIIAKHFVEAELRGHYSHGLQRVIPLVKGIDLGTIR
CEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTVQLTEIKREDNAVLYDANYSIGILVWHYLTESPKETLIAVRNSSHIGFLGYYTRRIAM
HHHHHHHHHCCCCEEEEECCCCEEEEEEEHHCCCCCCEEEEEECCCCEEHHHHHHHHHHH
RLGKPAIMIGNAEPAVVKPGTAKKIISTTPISIAIPCEKIVVLDMSLSPIARGKIIEAKR
HCCCCEEEECCCCCCEECCCCCHHHEECCCEEEEECCCEEEEEECCCCCCCCCCEEEHHH
KGEKIPYGVTVNKDGEITTDPDEALQGGLLPIGGMKGFFLMITLELLVSFLTGSALATEV
CCCCCCCEEEECCCCCEECCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHH
QGVLDTTKSPNKGEFMIILPRLQSDCHGLSVLKQFVEYLPGEHSDEMLKRDEIDIDEKLY
HHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHEE
NIFVKLEAEEQFNWTQNE
EEEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110059; 10850983 [H]