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The map label for this gene is rfbB [H]

Identifier: 15922289

GI number: 15922289

Start: 1982492

End: 1983454

Strand: Direct

Name: rfbB [H]

Synonym: ST1972

Alternate gene names: 15922289

Gene position: 1982492-1983454 (Clockwise)

Preceding gene: 15922288

Following gene: 15922290

Centisome position: 73.57

GC content: 39.46

Gene sequence:

>963_bases
ATGATAATTATTGGTGGTGCTGGTTTCATAGGCTCTGCGTTTGTAAGAGAGGTGAATAGGAGGGGAATTAAACCGGTTGT
GATTGATTTACTTACTTATGCCGGAAGGATGGAGAACTTAATTGGTACTAATCATGATTTCGTAAGGGCTGACATTAGGA
GTGAGGAGATTCATAATATTTTGAAAGAAAAAGAGTCTGCTAGTAATGTCGTTGTTAATTTTGCGGCTGAGACTCATGTG
GATAGGTCTATTTATAAGCCTCAGGATTTTGTGACTACTAATGTCATAGGAGTAGTGAACCTACTAGAAGCTGCTAGGAA
ATATGATTTTAATTATGTTCATATTTCTACTGATGAGGTTTATGGTGAGGAGTGTGGGGATGAGGATTCTCCTTTAAAAC
CATCTTCACCTTATAGTGCTTCTAAGGCTTCTGCTGATTTATTCGTTAAGGCTTATGTTAGGACTTACGGGATTAAGGCT
ATAATTATAAGGCCTTCAAACAACTACGGCCCTAGGCAGTTCCCAGAAAAGCTTATTCCTAAGGTGATAATTAGGACTTT
GATGGGGGAGTATGTCCCTATTTATGGTGATGGGAGAGCTGAGAGGGATTGGATTTATGTTGAGGATACTGCTAGGATAA
TTTATGATATTTTAGAAATGGCGGAATGGAGAGGGGAAGTTTATAATATTCCTGGAGGACAGAGGTATAGTGTGCTTGAC
GTTGTTAAGATGATCGGTGAGGTCATGGGGAGGGAGGTAAAAATTAAGTTTGTTAGTGATAGGCCCGGTCATGATAGGAG
GTATTGTATGATTACTAAGCTTAAGTATGAGGTTACGCCGTTAAGGGAGGGGTTGAGGAGGACTGTTGAGTGGTATTTGA
ATAATAGGTGGTGGTGGGAGCCATTGATAAAGGATAAGTTCTTTGTAGAAGATGAGCCTTGGAAAGTAAAAGTTAGTAAA
TAA

Upstream 100 bases:

>100_bases
GGGTTTCACTTATGGATTCGTTAATAGGGAACAATTCTGTCGTGGAGAAGGGAAATAGGTGGCAGAGGCTAATAATAGGT
GAGAATTCTTCGGTGATATT

Downstream 100 bases:

>100_bases
TGCTAATACAAAGTAGTATAAATTCTTAGCTAAAGGTTTTTGATTTTATATCTTAAATAGTTTGGTAAACTTCTAAAAAC
TACATCATCTTCCCTTTTCT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIIIGGAGFIGSAFVREVNRRGIKPVVIDLLTYAGRMENLIGTNHDFVRADIRSEEIHNILKEKESASNVVVNFAAETHV
DRSIYKPQDFVTTNVIGVVNLLEAARKYDFNYVHISTDEVYGEECGDEDSPLKPSSPYSASKASADLFVKAYVRTYGIKA
IIIRPSNNYGPRQFPEKLIPKVIIRTLMGEYVPIYGDGRAERDWIYVEDTARIIYDILEMAEWRGEVYNIPGGQRYSVLD
VVKMIGEVMGREVKIKFVSDRPGHDRRYCMITKLKYEVTPLREGLRRTVEWYLNNRWWWEPLIKDKFFVEDEPWKVKVSK

Sequences:

>Translated_320_residues
MIIIGGAGFIGSAFVREVNRRGIKPVVIDLLTYAGRMENLIGTNHDFVRADIRSEEIHNILKEKESASNVVVNFAAETHV
DRSIYKPQDFVTTNVIGVVNLLEAARKYDFNYVHISTDEVYGEECGDEDSPLKPSSPYSASKASADLFVKAYVRTYGIKA
IIIRPSNNYGPRQFPEKLIPKVIIRTLMGEYVPIYGDGRAERDWIYVEDTARIIYDILEMAEWRGEVYNIPGGQRYSVLD
VVKMIGEVMGREVKIKFVSDRPGHDRRYCMITKLKYEVTPLREGLRRTVEWYLNNRWWWEPLIKDKFFVEDEPWKVKVSK
>Mature_320_residues
MIIIGGAGFIGSAFVREVNRRGIKPVVIDLLTYAGRMENLIGTNHDFVRADIRSEEIHNILKEKESASNVVVNFAAETHV
DRSIYKPQDFVTTNVIGVVNLLEAARKYDFNYVHISTDEVYGEECGDEDSPLKPSSPYSASKASADLFVKAYVRTYGIKA
IIIRPSNNYGPRQFPEKLIPKVIIRTLMGEYVPIYGDGRAERDWIYVEDTARIIYDILEMAEWRGEVYNIPGGQRYSVLD
VVKMIGEVMGREVKIKFVSDRPGHDRRYCMITKLKYEVTPLREGLRRTVEWYLNNRWWWEPLIKDKFFVEDEPWKVKVSK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=32.9192546583851, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=25.3048780487805, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI56237023, Length=304, Percent_Identity=29.2763157894737, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI56118217, Length=304, Percent_Identity=29.2763157894737, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI189083684, Length=304, Percent_Identity=29.2763157894737, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=43.0678466076696, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=42.9824561403509, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=27.2455089820359, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788365, Length=329, Percent_Identity=24.0121580547112, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788366, Length=205, Percent_Identity=27.8048780487805, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=33.0246913580247, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=32.0987654320988, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17539532, Length=223, Percent_Identity=26.9058295964126, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=305, Percent_Identity=26.8852459016393, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=24.6835443037975, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19923002, Length=303, Percent_Identity=27.7227722772277, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36957; Mature: 36957

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIGGAGFIGSAFVREVNRRGIKPVVIDLLTYAGRMENLIGTNHDFVRADIRSEEIHNI
CEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
LKEKESASNVVVNFAAETHVDRSIYKPQDFVTTNVIGVVNLLEAARKYDFNYVHISTDEV
HHHHCCCCCEEEEEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
YGEECGDEDSPLKPSSPYSASKASADLFVKAYVRTYGIKAIIIRPSNNYGPRQFPEKLIP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHH
KVIIRTLMGEYVPIYGDGRAERDWIYVEDTARIIYDILEMAEWRGEVYNIPGGQRYSVLD
HHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEHHHH
VVKMIGEVMGREVKIKFVSDRPGHDRRYCMITKLKYEVTPLREGLRRTVEWYLNNRWWWE
HHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCCCEEEC
PLIKDKFFVEDEPWKVKVSK
HHHCCCEEECCCCCEEEECC
>Mature Secondary Structure
MIIIGGAGFIGSAFVREVNRRGIKPVVIDLLTYAGRMENLIGTNHDFVRADIRSEEIHNI
CEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
LKEKESASNVVVNFAAETHVDRSIYKPQDFVTTNVIGVVNLLEAARKYDFNYVHISTDEV
HHHHCCCCCEEEEEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
YGEECGDEDSPLKPSSPYSASKASADLFVKAYVRTYGIKAIIIRPSNNYGPRQFPEKLIP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHH
KVIIRTLMGEYVPIYGDGRAERDWIYVEDTARIIYDILEMAEWRGEVYNIPGGQRYSVLD
HHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEHHHH
VVKMIGEVMGREVKIKFVSDRPGHDRRYCMITKLKYEVTPLREGLRRTVEWYLNNRWWWE
HHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCCCEEEC
PLIKDKFFVEDEPWKVKVSK
HHHCCCEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]