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The map label for this gene is lpdA [H]

Identifier: 15922178

GI number: 15922178

Start: 1877227

End: 1878465

Strand: Direct

Name: lpdA [H]

Synonym: ST1865

Alternate gene names: 15922178

Gene position: 1877227-1878465 (Clockwise)

Preceding gene: 15922175

Following gene: 15922182

Centisome position: 69.66

GC content: 34.3

Gene sequence:

>1239_bases
ATGATTGTTGTTATAGGCTCTGGTCCTGCCGGAATATATGGTGCATTTACAGCTTCTGCTTTAGGAGAAAAAGTTAAGCT
TATCGAGGAAAAAGAAAGGTTAGGTGGAACTTGTGTTTTATATGGTTGTATTCCTTCAAAAGCAATGTTACATCCACTTT
ATTTAAAGTATTCTTTGGAAAGGTTGGGAAAGAAAATCGATTTTAGCTATGAAGAACTGATCAAGCTTGCCCAAGATGCG
ATTTCAAGGATCTCAAAAGGAGTTGAATACATGTTAGAAAGTTACGGTGTTGAAGTCATTTATGGTAGAGGAGTACTTAA
AAGCAAAGAAATTGAAGTTTCTGGTCAAACTTTATACCCAGATAAAGTACTAGTAGCAACTGGAACTTTAAAACCAGAAA
TTAATGGAACTATAGCTTCAGACGATTTGCCGTATCTTAATAAAGAGTTTAATTCAGTAACCGTAATTGGTGGTGGTGTA
GGGGGTATTGAATATGGCTGGTTGTTACACAAAATAGGGAAAGAAGTACATATAGTAGAGAAAGAAAATTTACTTTTACC
GAAACATGATATGGATTTAAGAAATTACGTAACTAACCACTTTAAGAGAATTGGAGTTAAACTTCATTTAGGAGTGGAGG
CTAAGATAGAGAATAATAAAGTTAAACTCTCGAATGGAGAAGAAATTAAGAGCGATATTATTTTAATGAGTTTTGGAAGA
AAACCTAATCTTACTGGGGTTGAAAGTCTACCTCATGATTATTGGATAAAGGTTAACGAGTACATGGAAACTCCGATAAA
AGACATTTACGCTGCTGGTGATATAACTGGTTCATTTACAGCACATGAAGCAATACATAAAGGATATATAGCTGGGCTTA
ATATGAAAGGAATCAAAAAACCTTATGATAGTTCTGCTGTTCCAAAGGTTATTTATACATCACCACAAATTGCTTACGTC
GGAAACACAAATAGTGGTAGATGTATGAAAATGGAATTTTCTGGGTTAGCTAGAGCTATTGCAGAAAAGGAGACTGAAGG
ATTTTTAAAGATCTGTGTGGAAAATGAAAAAGTAATAGGTGGTGTTGCATTTTCAGAAAGGGCTGAAGAAATTATCTCTC
TTTTGGCTTTAGGGATTAAGTTTAAGGGAAGTTTAGATGAGCTTCTCGATTTCCAGTATCCACATCCTTCTTATCTTGAA
ATGATATGGGAAATTTTAAGAAAAATTAAGTGGGGATAA

Upstream 100 bases:

>100_bases
TCAAAAAAGTTACTTATAAACCTTATTATATTCAGTTTGTGTACTAACTTTACATTTTTCTGTGATAAAGCTTAATTAGT
AGTAATAGATATCAAAATTC

Downstream 100 bases:

>100_bases
GGATCTTTTTCTCATCTAATTCTTTCAAGAATAAAGCATTTTGATAAACTTCGTTCTCATCAAACCGTAGCTCACCAAGA
AGTTTTTTACTTACGCTACA

Product: lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MIVVIGSGPAGIYGAFTASALGEKVKLIEEKERLGGTCVLYGCIPSKAMLHPLYLKYSLERLGKKIDFSYEELIKLAQDA
ISRISKGVEYMLESYGVEVIYGRGVLKSKEIEVSGQTLYPDKVLVATGTLKPEINGTIASDDLPYLNKEFNSVTVIGGGV
GGIEYGWLLHKIGKEVHIVEKENLLLPKHDMDLRNYVTNHFKRIGVKLHLGVEAKIENNKVKLSNGEEIKSDIILMSFGR
KPNLTGVESLPHDYWIKVNEYMETPIKDIYAAGDITGSFTAHEAIHKGYIAGLNMKGIKKPYDSSAVPKVIYTSPQIAYV
GNTNSGRCMKMEFSGLARAIAEKETEGFLKICVENEKVIGGVAFSERAEEIISLLALGIKFKGSLDELLDFQYPHPSYLE
MIWEILRKIKWG

Sequences:

>Translated_412_residues
MIVVIGSGPAGIYGAFTASALGEKVKLIEEKERLGGTCVLYGCIPSKAMLHPLYLKYSLERLGKKIDFSYEELIKLAQDA
ISRISKGVEYMLESYGVEVIYGRGVLKSKEIEVSGQTLYPDKVLVATGTLKPEINGTIASDDLPYLNKEFNSVTVIGGGV
GGIEYGWLLHKIGKEVHIVEKENLLLPKHDMDLRNYVTNHFKRIGVKLHLGVEAKIENNKVKLSNGEEIKSDIILMSFGR
KPNLTGVESLPHDYWIKVNEYMETPIKDIYAAGDITGSFTAHEAIHKGYIAGLNMKGIKKPYDSSAVPKVIYTSPQIAYV
GNTNSGRCMKMEFSGLARAIAEKETEGFLKICVENEKVIGGVAFSERAEEIISLLALGIKFKGSLDELLDFQYPHPSYLE
MIWEILRKIKWG
>Mature_412_residues
MIVVIGSGPAGIYGAFTASALGEKVKLIEEKERLGGTCVLYGCIPSKAMLHPLYLKYSLERLGKKIDFSYEELIKLAQDA
ISRISKGVEYMLESYGVEVIYGRGVLKSKEIEVSGQTLYPDKVLVATGTLKPEINGTIASDDLPYLNKEFNSVTVIGGGV
GGIEYGWLLHKIGKEVHIVEKENLLLPKHDMDLRNYVTNHFKRIGVKLHLGVEAKIENNKVKLSNGEEIKSDIILMSFGR
KPNLTGVESLPHDYWIKVNEYMETPIKDIYAAGDITGSFTAHEAIHKGYIAGLNMKGIKKPYDSSAVPKVIYTSPQIAYV
GNTNSGRCMKMEFSGLARAIAEKETEGFLKICVENEKVIGGVAFSERAEEIISLLALGIKFKGSLDELLDFQYPHPSYLE
MIWEILRKIKWG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=28.4463894967177, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=23.6051502145923, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=27.0509977827051, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789915, Length=429, Percent_Identity=27.7389277389277, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082354, Length=450, Percent_Identity=26.8888888888889, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=25.1111111111111, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32565766, Length=453, Percent_Identity=26.0485651214128, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=461, Percent_Identity=25.3796095444685, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983429, Length=430, Percent_Identity=25.8139534883721, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983419, Length=430, Percent_Identity=25.8139534883721, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=459, Percent_Identity=24.8366013071895, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=26.1538461538462, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6325240, Length=462, Percent_Identity=22.5108225108225, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=28.7912087912088, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24640551, Length=322, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640553, Length=322, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640549, Length=322, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 45702; Mature: 45702

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVIGSGPAGIYGAFTASALGEKVKLIEEKERLGGTCVLYGCIPSKAMLHPLYLKYSLE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
RLGKKIDFSYEELIKLAQDAISRISKGVEYMLESYGVEVIYGRGVLKSKEIEVSGQTLYP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCEEEECCCEECC
DKVLVATGTLKPEINGTIASDDLPYLNKEFNSVTVIGGGVGGIEYGWLLHKIGKEVHIVE
CEEEEEECCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEE
KENLLLPKHDMDLRNYVTNHFKRIGVKLHLGVEAKIENNKVKLSNGEEIKSDIILMSFGR
CCCEECCCCCCHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEECCCCHHHHCEEEEECCC
KPNLTGVESLPHDYWIKVNEYMETPIKDIYAAGDITGSFTAHEAIHKGYIAGLNMKGIKK
CCCCCCHHHCCCCEEEEEHHHHCCCHHHHHCCCCCCCCHHHHHHHHCCEEECCCCCCCCC
PYDSSAVPKVIYTSPQIAYVGNTNSGRCMKMEFSGLARAIAEKETEGFLKICVENEKVIG
CCCCCCCCEEEECCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCEEEE
GVAFSERAEEIISLLALGIKFKGSLDELLDFQYPHPSYLEMIWEILRKIKWG
EEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVVIGSGPAGIYGAFTASALGEKVKLIEEKERLGGTCVLYGCIPSKAMLHPLYLKYSLE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
RLGKKIDFSYEELIKLAQDAISRISKGVEYMLESYGVEVIYGRGVLKSKEIEVSGQTLYP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCEEEECCCEECC
DKVLVATGTLKPEINGTIASDDLPYLNKEFNSVTVIGGGVGGIEYGWLLHKIGKEVHIVE
CEEEEEECCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEE
KENLLLPKHDMDLRNYVTNHFKRIGVKLHLGVEAKIENNKVKLSNGEEIKSDIILMSFGR
CCCEECCCCCCHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEECCCCHHHHCEEEEECCC
KPNLTGVESLPHDYWIKVNEYMETPIKDIYAAGDITGSFTAHEAIHKGYIAGLNMKGIKK
CCCCCCHHHCCCCEEEEEHHHHCCCHHHHHCCCCCCCCHHHHHHHHCCEEECCCCCCCCC
PYDSSAVPKVIYTSPQIAYVGNTNSGRCMKMEFSGLARAIAEKETEGFLKICVENEKVIG
CCCCCCCCEEEECCCEEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCEEEE
GVAFSERAEEIISLLALGIKFKGSLDELLDFQYPHPSYLEMIWEILRKIKWG
EEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]