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The map label for this gene is proP [H]

Identifier: 15922140

GI number: 15922140

Start: 1839755

End: 1840960

Strand: Reverse

Name: proP [H]

Synonym: ST1828

Alternate gene names: 15922140

Gene position: 1840960-1839755 (Counterclockwise)

Preceding gene: 15922143

Following gene: 15922139

Centisome position: 68.32

GC content: 34.66

Gene sequence:

>1206_bases
ATGAACTGGAAAATAAACTTTCTTGGAGCTTATTTATCGTGGGTAATGGATTCTTATGATTTAGGGGCTGTAGTAATAAC
ATCAACAATTTTAGGGAAACTTTTTTACCCTACACTAGGTTTATTAGGTGCAGTTCTTCCAATAGTATTTACTGTTGTAT
TTAGACCAATAGGCTCATTAATTTTTGGACTATTTGCAGATTGGAAGGGAAGAAAATCAATTTTAACCATTACAGTTTTA
GGTTACTCCTTAGCAATAGGTTTAACGGCGTTTCTTCCTACATATTATCAAGCTGGAATATTGGCTACAGTACTCCTCAG
CATTTTAAGAGTTTTTCAGGGAATTTTTATCGGTGGGGATGTCTCATCAAGTTTTACGCTTGCAATGGAAAGCGTATTTA
CTCGTAGGGGGCTATTTTCTGGAATAACACAGTCTGGCACGTTGGTGGGATTTGTAATAGTAGACTTGCTTTTCACTTAC
TTTGCTAAGGATCCAACATTTCTAATAACTGAGGGTTGGAGAATAATATTTCTTGTAGGTATTCTACCGGCAATTTTAGC
TATAATAATACGGTATAAAGTTACAGAATCAGAAATTTACCTAAAATCTGAGAAAAAGCCTGTATCACAAGGATTGAGAC
CTATATTTCAAACAATTTTAGTAATGATAGGATTTTGGCTAATGATTTATTCTGGTCCTCAATTTGTACCAGTATTTTTA
GGTAACGTGTTAAAGTTACCTTCATCTGTTTACGGTTTCCTAGCACTGATAATGAACCTAATAGGGATTCCTGCGATGAT
TCTTTCGGGTTTCTTGTCAGACTACATTGGGAGAAAAAGTATGGCTATCATAGGGTCTATTCTCTCTATATTTGCTGGAA
TATTACTTTACCTTTACTCGGCAACGTCTAATCTAACTTTTCTCATAACGGCTTTTGGATTTTTAATTAATCTACCTTCA
GCGATTACTCCAGCCTATTTGGCTGAAAGGTTTAAGACATATAGTAGGGCTACTGGTGTAGGATTTTCTTATAACGGTGC
ATTTTTCGTTGCAGGCTTTGCACAAATATATATCTCTTTATTAGGTAAAATAATGCCAGTTAATTACTCAGCCTTAACGG
TATTAACAATTGGTGCTTTATTTGCTATAATTGGCTTGGCTATAGGACCAGAAACTCTAAAGGTAAATGAATTAAAAATA
AGTTAA

Upstream 100 bases:

>100_bases
ATTAATCCAAACATGATATCTTTTAATTAACATTACTTAATAAAACTACTCGTTATACCTTAAAACATAGTTTTTAAAAT
ATAAACTTGGTTTTCAATTT

Downstream 100 bases:

>100_bases
TTTTTTTCAAAACCTTTTCTTATTTCGACTCTTCTTTTCTATTATGAAATACACAAAACCCGTTTTAGTTGTCCATGGAG
GAGCTGGAGATTGGAGTAAA

Product: transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNWKINFLGAYLSWVMDSYDLGAVVITSTILGKLFYPTLGLLGAVLPIVFTVVFRPIGSLIFGLFADWKGRKSILTITVL
GYSLAIGLTAFLPTYYQAGILATVLLSILRVFQGIFIGGDVSSSFTLAMESVFTRRGLFSGITQSGTLVGFVIVDLLFTY
FAKDPTFLITEGWRIIFLVGILPAILAIIIRYKVTESEIYLKSEKKPVSQGLRPIFQTILVMIGFWLMIYSGPQFVPVFL
GNVLKLPSSVYGFLALIMNLIGIPAMILSGFLSDYIGRKSMAIIGSILSIFAGILLYLYSATSNLTFLITAFGFLINLPS
AITPAYLAERFKTYSRATGVGFSYNGAFFVAGFAQIYISLLGKIMPVNYSALTVLTIGALFAIIGLAIGPETLKVNELKI
S

Sequences:

>Translated_401_residues
MNWKINFLGAYLSWVMDSYDLGAVVITSTILGKLFYPTLGLLGAVLPIVFTVVFRPIGSLIFGLFADWKGRKSILTITVL
GYSLAIGLTAFLPTYYQAGILATVLLSILRVFQGIFIGGDVSSSFTLAMESVFTRRGLFSGITQSGTLVGFVIVDLLFTY
FAKDPTFLITEGWRIIFLVGILPAILAIIIRYKVTESEIYLKSEKKPVSQGLRPIFQTILVMIGFWLMIYSGPQFVPVFL
GNVLKLPSSVYGFLALIMNLIGIPAMILSGFLSDYIGRKSMAIIGSILSIFAGILLYLYSATSNLTFLITAFGFLINLPS
AITPAYLAERFKTYSRATGVGFSYNGAFFVAGFAQIYISLLGKIMPVNYSALTVLTIGALFAIIGLAIGPETLKVNELKI
S
>Mature_401_residues
MNWKINFLGAYLSWVMDSYDLGAVVITSTILGKLFYPTLGLLGAVLPIVFTVVFRPIGSLIFGLFADWKGRKSILTITVL
GYSLAIGLTAFLPTYYQAGILATVLLSILRVFQGIFIGGDVSSSFTLAMESVFTRRGLFSGITQSGTLVGFVIVDLLFTY
FAKDPTFLITEGWRIIFLVGILPAILAIIIRYKVTESEIYLKSEKKPVSQGLRPIFQTILVMIGFWLMIYSGPQFVPVFL
GNVLKLPSSVYGFLALIMNLIGIPAMILSGFLSDYIGRKSMAIIGSILSIFAGILLYLYSATSNLTFLITAFGFLINLPS
AITPAYLAERFKTYSRATGVGFSYNGAFFVAGFAQIYISLLGKIMPVNYSALTVLTIGALFAIIGLAIGPETLKVNELKI
S

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=384, Percent_Identity=27.34375, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788292, Length=374, Percent_Identity=25.9358288770053, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788942, Length=379, Percent_Identity=28.2321899736148, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082404, Length=394, Percent_Identity=27.4111675126904, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789941, Length=371, Percent_Identity=29.9191374663073, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43731; Mature: 43731

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKINFLGAYLSWVMDSYDLGAVVITSTILGKLFYPTLGLLGAVLPIVFTVVFRPIGSL
CCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFGLFADWKGRKSILTITVLGYSLAIGLTAFLPTYYQAGILATVLLSILRVFQGIFIGGD
HHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC
VSSSFTLAMESVFTRRGLFSGITQSGTLVGFVIVDLLFTYFAKDPTFLITEGWRIIFLVG
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
ILPAILAIIIRYKVTESEIYLKSEKKPVSQGLRPIFQTILVMIGFWLMIYSGPQFVPVFL
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GNVLKLPSSVYGFLALIMNLIGIPAMILSGFLSDYIGRKSMAIIGSILSIFAGILLYLYS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ATSNLTFLITAFGFLINLPSAITPAYLAERFKTYSRATGVGFSYNGAFFVAGFAQIYISL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LGKIMPVNYSALTVLTIGALFAIIGLAIGPETLKVNELKIS
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure
MNWKINFLGAYLSWVMDSYDLGAVVITSTILGKLFYPTLGLLGAVLPIVFTVVFRPIGSL
CCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFGLFADWKGRKSILTITVLGYSLAIGLTAFLPTYYQAGILATVLLSILRVFQGIFIGGD
HHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC
VSSSFTLAMESVFTRRGLFSGITQSGTLVGFVIVDLLFTYFAKDPTFLITEGWRIIFLVG
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
ILPAILAIIIRYKVTESEIYLKSEKKPVSQGLRPIFQTILVMIGFWLMIYSGPQFVPVFL
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GNVLKLPSSVYGFLALIMNLIGIPAMILSGFLSDYIGRKSMAIIGSILSIFAGILLYLYS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ATSNLTFLITAFGFLINLPSAITPAYLAERFKTYSRATGVGFSYNGAFFVAGFAQIYISL
HCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LGKIMPVNYSALTVLTIGALFAIIGLAIGPETLKVNELKIS
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]