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The map label for this gene is cbiHC [H]

Identifier: 15922135

GI number: 15922135

Start: 1835483

End: 1836244

Strand: Reverse

Name: cbiHC [H]

Synonym: ST1823

Alternate gene names: 15922135

Gene position: 1836244-1835483 (Counterclockwise)

Preceding gene: 15922136

Following gene: 15922134

Centisome position: 68.14

GC content: 35.3

Gene sequence:

>762_bases
ATGGCGGGTAAAATTTACATAATCGGTATTGGTCCTGGAGATGAAAAAAACCGTACTTTAAGAATGCTAGAGGCTATAAA
AGAAAGTGACGTTATAATTGCGTACACGACTTACGCTGATTTAATTAAGGATTTAACCGATGGAAAAGAGGTTATCACAG
CAAGGATGAAAGAGGAATTATATAGGGCTAAAATGGCAATAAAGAAAGCTTTGGAGGGTCATACTGTTGCTTTAGTTTCT
AGTGGTGATCCCCAAGTTTATGGAATGGCACCACCAACACTTGAGATGATGTGTGCTAATAATGTTAACGTTGACTTTGA
AATTATACCAGGTGTTACTGCAGCTTTAGCTGCAGCAGCAAGATTAGGTTCACCATTATCAATGGATTTTGCAGTGATTA
GCTTAAGCGACCTTTTAGTACCTGCAGAAGAAATTCTTCATAGAGTTAGGAAAGCTGCTGAAGGTGACTTTGTAATTGCT
CTTTACAATCCTATAAATAAGCCTTTACTTTTAAAGGCGATGGAGATAATAGCTGAATATAGAAAAGGCGAAACACCAGT
TGGTATAGTAAAAGGTGCTTATAGGGAAAATGAGGAAGTTATCGTAACTACTTTATCAGAGTGGAAAAAATATCTTGATA
AAATAAATATGGTTACTATGATGATTATTGGTAATTCTCGTTCATATATATGTAATGGTAAAATAATAACTCCAAGAGGT
TATACGTCGAGGTATGATTATGTCAATGCTTTCAACTCTTAA

Upstream 100 bases:

>100_bases
ATAAGATCTGGCATACCATGTATAGTGTTAAGAGGAACTTACGGTGGTAGTAGTATAGCAGTATCAATCATAAATGAGCT
AATTAGAATGGTGAGGAGTT

Downstream 100 bases:

>100_bases
AAGATTTGGAATAACAACCGGGGCTACTGCAGCGGCATCAGCAAAGGCCTCTGTTATCTATTTATTTCGTAATGAAACTC
CTAAAAGTGTTACAATTCCA

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAGKIYIIGIGPGDEKNRTLRMLEAIKESDVIIAYTTYADLIKDLTDGKEVITARMKEELYRAKMAIKKALEGHTVALVS
SGDPQVYGMAPPTLEMMCANNVNVDFEIIPGVTAALAAAARLGSPLSMDFAVISLSDLLVPAEEILHRVRKAAEGDFVIA
LYNPINKPLLLKAMEIIAEYRKGETPVGIVKGAYRENEEVIVTTLSEWKKYLDKINMVTMMIIGNSRSYICNGKIITPRG
YTSRYDYVNAFNS

Sequences:

>Translated_253_residues
MAGKIYIIGIGPGDEKNRTLRMLEAIKESDVIIAYTTYADLIKDLTDGKEVITARMKEELYRAKMAIKKALEGHTVALVS
SGDPQVYGMAPPTLEMMCANNVNVDFEIIPGVTAALAAAARLGSPLSMDFAVISLSDLLVPAEEILHRVRKAAEGDFVIA
LYNPINKPLLLKAMEIIAEYRKGETPVGIVKGAYRENEEVIVTTLSEWKKYLDKINMVTMMIIGNSRSYICNGKIITPRG
YTSRYDYVNAFNS
>Mature_252_residues
AGKIYIIGIGPGDEKNRTLRMLEAIKESDVIIAYTTYADLIKDLTDGKEVITARMKEELYRAKMAIKKALEGHTVALVSS
GDPQVYGMAPPTLEMMCANNVNVDFEIIPGVTAALAAAARLGSPLSMDFAVISLSDLLVPAEEILHRVRKAAEGDFVIAL
YNPINKPLLLKAMEIIAEYRKGETPVGIVKGAYRENEEVIVTTLSEWKKYLDKINMVTMMIIGNSRSYICNGKIITPRGY
TSRYDYVNAFNS

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=25.5230125523013, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363 [H]

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 27932; Mature: 27801

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKIYIIGIGPGDEKNRTLRMLEAIKESDVIIAYTTYADLIKDLTDGKEVITARMKEEL
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHH
YRAKMAIKKALEGHTVALVSSGDPQVYGMAPPTLEMMCANNVNVDFEIIPGVTAALAAAA
HHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHH
RLGSPLSMDFAVISLSDLLVPAEEILHRVRKAAEGDFVIALYNPINKPLLLKAMEIIAEY
HCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
RKGETPVGIVKGAYRENEEVIVTTLSEWKKYLDKINMVTMMIIGNSRSYICNGKIITPRG
HCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCEEEEEEEECCCCEEECCEEECCCC
YTSRYDYVNAFNS
CCCCCCHHHCCCC
>Mature Secondary Structure 
AGKIYIIGIGPGDEKNRTLRMLEAIKESDVIIAYTTYADLIKDLTDGKEVITARMKEEL
CCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHH
YRAKMAIKKALEGHTVALVSSGDPQVYGMAPPTLEMMCANNVNVDFEIIPGVTAALAAAA
HHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHHHH
RLGSPLSMDFAVISLSDLLVPAEEILHRVRKAAEGDFVIALYNPINKPLLLKAMEIIAEY
HCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
RKGETPVGIVKGAYRENEEVIVTTLSEWKKYLDKINMVTMMIIGNSRSYICNGKIITPRG
HCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCEEEEEEEECCCCEEECCEEECCCC
YTSRYDYVNAFNS
CCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]