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The map label for this gene is mdh [H]

Identifier: 15922124

GI number: 15922124

Start: 1827224

End: 1828159

Strand: Direct

Name: mdh [H]

Synonym: ST1811

Alternate gene names: 15922124

Gene position: 1827224-1828159 (Clockwise)

Preceding gene: 15922121

Following gene: 15922125

Centisome position: 67.81

GC content: 35.68

Gene sequence:

>936_bases
ATGATAAACATGACAAAGATAGCTTTTATAGGAGTTGGAAAGATAGGTCAGACAATCGCCTTTAATACTATAATGGACGG
TTACGCTGATGAGGTCATGATCTACGATATTATCCCAGAATTACCGGAGAAATTTGAACATGAACTTAGACATGCTCTTG
CATCTAAAAGACTTAAAGTTGAACTTTTATCTACAAACAACTTAGACGATGTTGCTGGTGCTGATATTGTAGTTATTACT
GCTGGTAAACCAAGAAAACCTGGTATGAGTAGAAGAGATCTGTTTGTAGATAATGCTAAAATAATGATGGATCTAGCTAA
CAAATTACCTAAGAAGAATCATGGAGCCGTTTATATTATGGTTTCCAACCCGGTAGACATGATGGCTTCTGTATTCGCAA
GATATTCTAGGGAATTCGTAATTAGTACTGGAGACCAAGTAGAGACAATGAGGTTAAGAGCATATATAGCTAAAAAACTA
AAGATACCAGTTTATAGAGTTAATGGGTTTGTAGGTGGAGAGCATGGTGAAGATGCTGTAGTATTATGGAGTACTGTAAC
AGTTAATGGAAAACCATTCTCAGAGGATTTAGGAGTTACAAAGGCAGAGGTTGAAGATTATGTAAAGAAGATCCCAGGAG
AGATAATTAGAGTTATGGGAGGTACTACATGGGGTCCTGGGACTATTATAGCTGAATTAATAAGAGCAGTAGCACTCAAT
GAGAATAAAGTTATGTCAATAGCTACTCCAAGACAATTTGAAGACGAAATAATTCACGTTAGTGTGCCAACAGTAGTTGG
AAGTTCAATTGGTCCTTCATTAGAAAACTTATTAGATGAAAAGGACAGATGGAATTTAATGGCTTCAATGAAAGACTTCT
ATAATGTATATAAAGAGAATTTGAAACACCTAGAAACTTCTATCCAAGCACAGTAA

Upstream 100 bases:

>100_bases
ATTCGGCTATTTTATCTGAAAGTTCCATAACTTTCTCTTTGAAACTACCAATATAAACTTTTTGAGATAAATTTAAATAA
TCAAATAGAGAATTTTAAAC

Downstream 100 bases:

>100_bases
AATCTATTATAAGCTCATCACTTATTTTTTCTTGTGAAAATACTACTTGTTGATTACGATAATACGCTTTTCAACACCAA
TGAATGTGTAAAAGCATCAG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MINMTKIAFIGVGKIGQTIAFNTIMDGYADEVMIYDIIPELPEKFEHELRHALASKRLKVELLSTNNLDDVAGADIVVIT
AGKPRKPGMSRRDLFVDNAKIMMDLANKLPKKNHGAVYIMVSNPVDMMASVFARYSREFVISTGDQVETMRLRAYIAKKL
KIPVYRVNGFVGGEHGEDAVVLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALN
ENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMASMKDFYNVYKENLKHLETSIQAQ

Sequences:

>Translated_311_residues
MINMTKIAFIGVGKIGQTIAFNTIMDGYADEVMIYDIIPELPEKFEHELRHALASKRLKVELLSTNNLDDVAGADIVVIT
AGKPRKPGMSRRDLFVDNAKIMMDLANKLPKKNHGAVYIMVSNPVDMMASVFARYSREFVISTGDQVETMRLRAYIAKKL
KIPVYRVNGFVGGEHGEDAVVLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALN
ENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMASMKDFYNVYKENLKHLETSIQAQ
>Mature_311_residues
MINMTKIAFIGVGKIGQTIAFNTIMDGYADEVMIYDIIPELPEKFEHELRHALASKRLKVELLSTNNLDDVAGADIVVIT
AGKPRKPGMSRRDLFVDNAKIMMDLANKLPKKNHGAVYIMVSNPVDMMASVFARYSREFVISTGDQVETMRLRAYIAKKL
KIPVYRVNGFVGGEHGEDAVVLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALN
ENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMASMKDFYNVYKENLKHLETSIQAQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI15082234, Length=280, Percent_Identity=25.7142857142857, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI47059044, Length=280, Percent_Identity=26.4285714285714, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI221136809, Length=280, Percent_Identity=26.4285714285714, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=27.0967741935484, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=27.0967741935484, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=26.8211920529801, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=26.8211920529801, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI9257228, Length=282, Percent_Identity=26.9503546099291, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4504973, Length=282, Percent_Identity=26.9503546099291, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI260099727, Length=202, Percent_Identity=29.2079207920792, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260099725, Length=202, Percent_Identity=29.2079207920792, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI207028494, Length=218, Percent_Identity=26.605504587156, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=223, Percent_Identity=29.1479820627803, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=266, Percent_Identity=27.4436090225564, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17554310, Length=205, Percent_Identity=30.2439024390244, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136226, Length=279, Percent_Identity=26.5232974910394, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550422, Length=306, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24647881, Length=188, Percent_Identity=29.7872340425532, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34603; Mature: 34603

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINMTKIAFIGVGKIGQTIAFNTIMDGYADEVMIYDIIPELPEKFEHELRHALASKRLKV
CCCEEEEEEEECCCCCCCEEHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
ELLSTNNLDDVAGADIVVITAGKPRKPGMSRRDLFVDNAKIMMDLANKLPKKNHGAVYIM
EEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEE
VSNPVDMMASVFARYSREFVISTGDQVETMRLRAYIAKKLKIPVYRVNGFVGGEHGEDAV
ECCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCEECCCCCCCEE
VLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALN
EEEEEEEECCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
ENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMASMKDFYNVYKEN
CCCEEEEECCCCCCCCEEEEEECHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LKHLETSIQAQ
HHHHHHHHCCC
>Mature Secondary Structure
MINMTKIAFIGVGKIGQTIAFNTIMDGYADEVMIYDIIPELPEKFEHELRHALASKRLKV
CCCEEEEEEEECCCCCCCEEHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
ELLSTNNLDDVAGADIVVITAGKPRKPGMSRRDLFVDNAKIMMDLANKLPKKNHGAVYIM
EEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEE
VSNPVDMMASVFARYSREFVISTGDQVETMRLRAYIAKKLKIPVYRVNGFVGGEHGEDAV
ECCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCEECCCCCCCEE
VLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALN
EEEEEEEECCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
ENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMASMKDFYNVYKEN
CCCEEEEECCCCCCCCEEEEEECHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LKHLETSIQAQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]