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The map label for this gene is mcl2 [H]

Identifier: 15922098

GI number: 15922098

Start: 1803548

End: 1804306

Strand: Direct

Name: mcl2 [H]

Synonym: ST1786

Alternate gene names: 15922098

Gene position: 1803548-1804306 (Clockwise)

Preceding gene: 15922097

Following gene: 15922106

Centisome position: 66.93

GC content: 33.07

Gene sequence:

>759_bases
ATGATTAAGAAAACTGAAAGTATAAACGCAGACTCTTTTATTTTTGATTTAGAGGATGCTGTACCTACACAAGAAAAAGA
TAAGGCTAGAGAACTACTCTTATCTCTTTTGCCCTCATTAAACATTAAAAGAAGCTTAATTTGCGTTAGAATCAACTCGC
TCTCAACTAAAGAATCTATAAAAGATCTTGATTTTGTGATAAAAAGTGATATTGATTGTATAGTTATTCCAAAAGCCGAA
ATTGATCTTTCTTTCCTTCATAAAATGACTGGAAAGAAAGTTTTACCAATAATAGAAACAGCAAAGGGTCTAGTTAAAAT
TGAAGATATAATTAGGAGTGAAGGAATAGTAGGAATAAGTTGGGGTGCGGCTGACTTAGCTGATAGTTTACACGCTAATT
TGCCTAAGATAGAAGGAAGTGACTATATAAGACTGAAAATTGTAAGCATAGCTAGGACATACGGTATTCCTCCAATTGAT
AAAGTATTTTTTGATGTGAAAGACCTAGAGAGCTTTAGGAAAGAGTGTGAATTAGCAAGAGCTTTTGGTTTTGAAGGAAA
ACAAGTTATTCATCCTAATCAAGTCACAATAGCTAACGAAGTGTTTTCCCCAAGTAAAGAGGAAATAGAATGGGCAAAAA
AAGTAGTGGAAGAATACGAAAAATACGCTTCTGCAGGAAGGGGTGCAATAACTATAGATGGTAAATTAGTAGATGCTGTT
CATTATAGAATTGCGAAAAGAATTTTAGAGTCATCATAA

Upstream 100 bases:

>100_bases
TGATGAAATTGAAAAAGTTGTAAAATCCTTAAGTTAAGATTTTTTATAGTGACCCCTCTTGTTAAGATCACAACTTTATG
TCCCGGGTAATAATGAGAAA

Downstream 100 bases:

>100_bases
TATAACCTTACCCTCATATATACTCTTTATTCTTTCCCACATTCTATCTATATATTCTTCAAGACCTTTCACAATTTCTG
GTGAAACCCCCTTATATCTA

Product: citrate lyase subunit beta

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIKKTESINADSFIFDLEDAVPTQEKDKARELLLSLLPSLNIKRSLICVRINSLSTKESIKDLDFVIKSDIDCIVIPKAE
IDLSFLHKMTGKKVLPIIETAKGLVKIEDIIRSEGIVGISWGAADLADSLHANLPKIEGSDYIRLKIVSIARTYGIPPID
KVFFDVKDLESFRKECELARAFGFEGKQVIHPNQVTIANEVFSPSKEEIEWAKKVVEEYEKYASAGRGAITIDGKLVDAV
HYRIAKRILESS

Sequences:

>Translated_252_residues
MIKKTESINADSFIFDLEDAVPTQEKDKARELLLSLLPSLNIKRSLICVRINSLSTKESIKDLDFVIKSDIDCIVIPKAE
IDLSFLHKMTGKKVLPIIETAKGLVKIEDIIRSEGIVGISWGAADLADSLHANLPKIEGSDYIRLKIVSIARTYGIPPID
KVFFDVKDLESFRKECELARAFGFEGKQVIHPNQVTIANEVFSPSKEEIEWAKKVVEEYEKYASAGRGAITIDGKLVDAV
HYRIAKRILESS
>Mature_252_residues
MIKKTESINADSFIFDLEDAVPTQEKDKARELLLSLLPSLNIKRSLICVRINSLSTKESIKDLDFVIKSDIDCIVIPKAE
IDLSFLHKMTGKKVLPIIETAKGLVKIEDIIRSEGIVGISWGAADLADSLHANLPKIEGSDYIRLKIVSIARTYGIPPID
KVFFDVKDLESFRKECELARAFGFEGKQVIHPNQVTIANEVFSPSKEEIEWAKKVVEEYEKYASAGRGAITIDGKLVDAV
HYRIAKRILESS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=263, Percent_Identity=31.9391634980989, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI87081764, Length=256, Percent_Identity=29.296875, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557458, Length=269, Percent_Identity=26.7657992565056, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28173; Mature: 28173

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTESINADSFIFDLEDAVPTQEKDKARELLLSLLPSLNIKRSLICVRINSLSTKESI
CCCCCCCCCCCCEEEECHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
KDLDFVIKSDIDCIVIPKAEIDLSFLHKMTGKKVLPIIETAKGLVKIEDIIRSEGIVGIS
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
WGAADLADSLHANLPKIEGSDYIRLKIVSIARTYGIPPIDKVFFDVKDLESFRKECELAR
CCHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AFGFEGKQVIHPNQVTIANEVFSPSKEEIEWAKKVVEEYEKYASAGRGAITIDGKLVDAV
HHCCCCCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
HYRIAKRILESS
HHHHHHHHHHCC
>Mature Secondary Structure
MIKKTESINADSFIFDLEDAVPTQEKDKARELLLSLLPSLNIKRSLICVRINSLSTKESI
CCCCCCCCCCCCEEEECHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
KDLDFVIKSDIDCIVIPKAEIDLSFLHKMTGKKVLPIIETAKGLVKIEDIIRSEGIVGIS
HHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
WGAADLADSLHANLPKIEGSDYIRLKIVSIARTYGIPPIDKVFFDVKDLESFRKECELAR
CCHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AFGFEGKQVIHPNQVTIANEVFSPSKEEIEWAKKVVEEYEKYASAGRGAITIDGKLVDAV
HHCCCCCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHH
HYRIAKRILESS
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA