BacMap

Definition	Sulfolobus tokodaii str. 7 chromosome, complete genome.
Accession	NC_003106
Length	2,694,756

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The map label for this gene is paaF [C]

Identifier: 15921809

GI number: 15921809

Start: 1510794

End: 1511603

Strand: Reverse

Name: paaF [C]

Synonym: ST1516

Alternate gene names: 15921809

Gene position: 1511603-1510794 (Counterclockwise)

Preceding gene: 15921811

Following gene: 15921806

Centisome position: 56.09

GC content: 33.09

Gene sequence:

>810_bases
ATGTTTATTTTTAAGCTTAATCTCTATGAAGATCTTATGGAAACAATTGTTATAAAAAAAGAGACCCCAATAGGGTGGAT
TTATTTAAATAGACCAGATAGATTAAATGCTATAAATCAACAAATGATAAAAGAATTGAGACAAGGAATAGACGAAATGG
TATATGACTCTGACATTAAAGTAATTATAATTACTGGAAACGGAAAAGCTTTTTCAGCTGGTGCTGATATTTCTCGATTT
AAAGAGCTAAATGGCTATACTGCTTGGCAATTTGCTAAGAGTGGGAGAGAACTTATGGATTATATAGAAAATATTAGTAA
ACCTACAATAGCAATGGTAAATGGTTATGCTTTAGGGGGAGGACTAGAATTAGCAATGGCTTGTGATATACGAATTGCTG
CTGAAGAAGCGCAATTAGGCTTACCAGAAATTAACTTAGGAATTTATCCGGGTTTTGGAGGAACACAAAGATTAGTTAGG
CTAATAGGAAAAGGAAAAGCATTAGAACTAATGTTAACTGGAGATAGAATTTCAGCTAAGGAGGCTGAAAAAATTGGTTT
AGTTAACAAAGTAGTCCCATTAAGTAACCTAGAACAAGAAACTAGGAATTTTGCACTTAAGTTAGCTGAAAAACCACCAA
TCTCTATTGCATTAATAAAACTCCTTGTTAATCAAGGAATAGATTTACCAATTTTAGCTGGTTTAAATATGGAAAGCTTA
GGATGGGGTGTTGTTTTTAGTACTGAAGATGAGAAAGAAGGTGTAAGTGCTTTCCTAGAGAAAAGAAAGGCTCAGTTTAA
GGGTAAATAG

Upstream 100 bases:

>100_bases
TCGCAATAGAGGAATATAATAGTGGTCTAATATCTTTTTTCATAATTATCTTTACTGATCAAGATCTTAAAAGCCTTTTT
CAAAGACTTTTAGCTTAAGC

Downstream 100 bases:

>100_bases
TATCTCTTCTTTTTTAATTAAATATATACTGATTCAAACCTATCTTCAAATGCATGCTTTGAAAGTTTACCAACTATTGG
TAACTTATAACCACAGTATT

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFIFKLNLYEDLMETIVIKKETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIKVIIITGNGKAFSAGADISRF
KELNGYTAWQFAKSGRELMDYIENISKPTIAMVNGYALGGGLELAMACDIRIAAEEAQLGLPEINLGIYPGFGGTQRLVR
LIGKGKALELMLTGDRISAKEAEKIGLVNKVVPLSNLEQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESL
GWGVVFSTEDEKEGVSAFLEKRKAQFKGK

Sequences:

>Translated_269_residues
MFIFKLNLYEDLMETIVIKKETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIKVIIITGNGKAFSAGADISRF
KELNGYTAWQFAKSGRELMDYIENISKPTIAMVNGYALGGGLELAMACDIRIAAEEAQLGLPEINLGIYPGFGGTQRLVR
LIGKGKALELMLTGDRISAKEAEKIGLVNKVVPLSNLEQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESL
GWGVVFSTEDEKEGVSAFLEKRKAQFKGK
>Mature_269_residues
MFIFKLNLYEDLMETIVIKKETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIKVIIITGNGKAFSAGADISRF
KELNGYTAWQFAKSGRELMDYIENISKPTIAMVNGYALGGGLELAMACDIRIAAEEAQLGLPEINLGIYPGFGGTQRLVR
LIGKGKALELMLTGDRISAKEAEKIGLVNKVVPLSNLEQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESL
GWGVVFSTEDEKEGVSAFLEKRKAQFKGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=44.8, Blast_Score=207, Evalue=6e-54,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=40.4651162790698, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=36.9918699186992, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=34.4398340248963, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI20127408, Length=232, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=30.5555555555556, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=28.6245353159851, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=30.2127659574468, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=30.2127659574468, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=29.4930875576037, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=29.4930875576037, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=29.4930875576037, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI37594469, Length=232, Percent_Identity=30.6034482758621, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI37594471, Length=232, Percent_Identity=30.6034482758621, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI261878539, Length=124, Percent_Identity=45.9677419354839, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4757966, Length=239, Percent_Identity=30.1255230125523, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI51538581, Length=239, Percent_Identity=30.1255230125523, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI25453481, Length=239, Percent_Identity=30.1255230125523, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI51538573, Length=239, Percent_Identity=30.1255230125523, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694520, Length=204, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4757968, Length=245, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI49355787, Length=245, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI221136756, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136753, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=213, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI116235487, Length=190, Percent_Identity=26.8421052631579, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=39.1472868217054, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=35.361216730038, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=35.7142857142857, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=38.7640449438202, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=38.1443298969072, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=33.206106870229, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=31.2, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=42.1052631578947, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=39.8406374501992, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=34.8017621145374, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=37.17277486911, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=31.5555555555556, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17508953, Length=224, Percent_Identity=34.375, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=28.1481481481481, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=224, Percent_Identity=34.375, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17508951, Length=224, Percent_Identity=34.375, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=29.0836653386454, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=29.3877551020408, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=26.8774703557312, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=236, Percent_Identity=28.8135593220339, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=32.4742268041237, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=32.4742268041237, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25141363, Length=190, Percent_Identity=29.4736842105263, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320241, Length=220, Percent_Identity=28.6363636363636, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=46.9387755102041, Blast_Score=203, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=46.9387755102041, Blast_Score=203, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=37.8486055776892, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=31.5270935960591, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920382, Length=264, Percent_Identity=26.8939393939394, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=24.5136186770428, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21357171, Length=180, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921000, Length=209, Percent_Identity=33.4928229665072, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24583077, Length=209, Percent_Identity=33.4928229665072, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583079, Length=209, Percent_Identity=33.4928229665072, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=27.363184079602, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571729, Length=176, Percent_Identity=32.3863636363636, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=176, Percent_Identity=32.3863636363636, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583165, Length=180, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921018, Length=187, Percent_Identity=27.807486631016, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29767; Mature: 29767

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIFKLNLYEDLMETIVIKKETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIK
CEEEEECHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VIIITGNGKAFSAGADISRFKELNGYTAWQFAKSGRELMDYIENISKPTIAMVNGYALGG
EEEEECCCCEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECC
GLELAMACDIRIAAEEAQLGLPEINLGIYPGFGGTQRLVRLIGKGKALELMLTGDRISAK
CEEEEEEEEEEEEECHHHCCCCEECEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCHH
EAEKIGLVNKVVPLSNLEQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESL
HHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GWGVVFSTEDEKEGVSAFLEKRKAQFKGK
CCEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFIFKLNLYEDLMETIVIKKETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIK
CEEEEECHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VIIITGNGKAFSAGADISRFKELNGYTAWQFAKSGRELMDYIENISKPTIAMVNGYALGG
EEEEECCCCEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECC
GLELAMACDIRIAAEEAQLGLPEINLGIYPGFGGTQRLVRLIGKGKALELMLTGDRISAK
CEEEEEEEEEEEEECHHHCCCCEECEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCHH
EAEKIGLVNKVVPLSNLEQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESL
HHHHHHHHHHCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GWGVVFSTEDEKEGVSAFLEKRKAQFKGK
CCEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA