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The map label for this gene is metG [H]

Identifier: 15921785

GI number: 15921785

Start: 1487332

End: 1487673

Strand: Reverse

Name: metG [H]

Synonym: ST1492

Alternate gene names: 15921785

Gene position: 1487673-1487332 (Counterclockwise)

Preceding gene: 15921800

Following gene: 15921784

Centisome position: 55.21

GC content: 34.21

Gene sequence:

>342_bases
ATGAGTGAAATTACAATAGACGACTTTGCAAAATTAGATTTAAGAGTAGGAATTGTAAAGTCAGCTGAGAGGATAGAAGG
AACTAGATTACTAAAATTAATCGTTGATTTAGGCAGTGAATCTAGGCAGATTATTTCTGGTATAGCTGAATTTTACACGC
CAGAAGAATTAGTGGGAAAAAGAGTCATACTCATAGCTAATTTAAAGCCTAAAAAAATAAGAGGATTTGAAAGTCAGGGA
ATGATATTAGCTGCTGGTTGTAAAGAAGATGAGGAGAAAGGAATAAAACCTAGAATACTTACAGTAGATGGGGAAGTTCC
CGCAGGAACAAAAGTATGTTAA

Upstream 100 bases:

>100_bases
TTTACATGAGCAGTATAAAAAACTTGTGTTAATAACCTAAGTATTTTTACTTAGCTAAGTAAACGAATACTCATTATCTA
GTTCATTTTACTTAACCCCT

Downstream 100 bases:

>100_bases
ACCCCTTTGAAAATATAAAAATACCACCAAAGACAGAGGACTTAATAAAAATAGTCCTAGATAGGATACCTAAAATAGGA
GGAAAAAATGTAAAGGAGAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MSEITIDDFAKLDLRVGIVKSAERIEGTRLLKLIVDLGSESRQIISGIAEFYTPEELVGKRVILIANLKPKKIRGFESQG
MILAAGCKEDEEKGIKPRILTVDGEVPAGTKVC

Sequences:

>Translated_113_residues
MSEITIDDFAKLDLRVGIVKSAERIEGTRLLKLIVDLGSESRQIISGIAEFYTPEELVGKRVILIANLKPKKIRGFESQG
MILAAGCKEDEEKGIKPRILTVDGEVPAGTKVC
>Mature_112_residues
SEITIDDFAKLDLRVGIVKSAERIEGTRLLKLIVDLGSESRQIISGIAEFYTPEELVGKRVILIANLKPKKIRGFESQGM
ILAAGCKEDEEKGIKPRILTVDGEVPAGTKVC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=85, Percent_Identity=50.5882352941176, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1789455, Length=113, Percent_Identity=33.6283185840708, Blast_Score=70, Evalue=2e-14,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12369; Mature: 12238

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEITIDDFAKLDLRVGIVKSAERIEGTRLLKLIVDLGSESRQIISGIAEFYTPEELVGK
CCCCCCCCHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCC
RVILIANLKPKKIRGFESQGMILAAGCKEDEEKGIKPRILTVDGEVPAGTKVC
EEEEEECCCCHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SEITIDDFAKLDLRVGIVKSAERIEGTRLLKLIVDLGSESRQIISGIAEFYTPEELVGK
CCCCCCCHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCC
RVILIANLKPKKIRGFESQGMILAAGCKEDEEKGIKPRILTVDGEVPAGTKVC
EEEEEECCCCHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]