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The map label for this gene is proS

Identifier: 15921730

GI number: 15921730

Start: 1444415

End: 1445866

Strand: Direct

Name: proS

Synonym: ST1440

Alternate gene names: 15921730

Gene position: 1444415-1445866 (Clockwise)

Preceding gene: 15921729

Following gene: 161751093

Centisome position: 53.6

GC content: 33.54

Gene sequence:

>1452_bases
ATGAAAGTTTCCAAGGAAAAGTGGGAGAAAAATTTTAGTGAATGGTTAGATTGGGTATTAAGAGAGGCTGAGATTTACGA
TTATGGCCGATATCCCGTAAAAGGTATGGGGGTATGGATGCCATATGGTTTCAAGATAAGGCAAAATGTGCTTCAACTTA
TCAGAAAATTATTAGATGAAACAGGACATGAAGAAGTTTTATTTCCATTACTTATTCCAGAAGATCTATTGAAAAAAGAA
AGTGAACACATAAGGGGATTTGAGGAAGAAGTCTATTGGGTAACTAAGGGTGGTTCTCAAGATCTAGATGTAAAATTAGC
ATTAAGACCTACCAGTGAAACTTCTATAACGTTTATGGAATCTTTTTGGGTTAAAAGTTACAAACAATTACCTAAAAAAT
ATTACCAAATTGTTAGTGTATTTAGATACGAGACTAAAGCTACAAGGCCTATGATGAGATTAAGGGAAATAACAACTTTC
AAGGAAGCTCATACACTTCACGAAACATATGAAGATGCGGCTAGGCAAGTAGACGAAGCTATAAATATATATAAAGCGTT
CTTTGATGAGCTTGGAATTCCATATATGATTTCTAAAAGACCAGAATGGGATAAATTTGCTGGAGCTGAGTACACTATAG
CTTTTGATACTATATTACCAGATTCGAGAGTTTTACAAATAGGTACTGTGCATCATTTAGGTCAGCACTTTACGAAGGCT
TTTGATTTCAAAATCCAAAGAAAAGATGGAAGTTTAGATTATCCTCATCAAACTAGTTATGGGATTTCAGATAGAGTAAT
AGCAGTACTTATAGCTATAAATGGTGATGATCATGGTCCAGTACTAAACCCTGTAATAGCACCAATAAAGGTAGTTATAG
TTCCTATACCAGCTAAGGATGAAGAAACAACTGCTAAAATTATTAACTATGCTAAAGAAGTTGGAGAGAATCTAAAGAAT
AGAGGCATTACTGTGGTAATAGATGATGATAAAGAGAAAACACCTGGTGAGAAGTTCTATATATGGGAATTAAAAGGAGT
TCCTTTAAGAATTGAAATAGGCCCTAAGGAACTAAATAATAATACTGTTTATATTAAAAGAAGGGATACTTTTGAAGGTA
AATCAGTGCCTAAAGATAAGGCAGTAGAGGAGGTAAATACTCTATTAGAGAAAATAAAGAATGATTTACATGAGAAGGCG
TTAAAGTTCTTAAAAGAGAGGATAATATATACTGAAGACCTTAATGAAGCTAAAAAGATTTTAGAAGAAAGAGCTGGCGT
TGTTGAAGTACCATGGTGTGGTGATAATAATTGCGGCTTGCAACTTCAGGATGTAACTAATGCTAGAGTTTTGGGTATTC
CTCTTGATGAGGATAAAGATGTTAGTAATGCAAAATGTGTAATGTGTAAAAAACCAGCAAAATCATTACTTAGGTTGGCA
AAAACTTATTAA

Upstream 100 bases:

>100_bases
AATAGCCGTATACGGTATCTTGTTCGCGGTATTAATGCTATTCGGTAAGTTCTAAAAGACAAAAATGTTTTTTATCTTCT
TCTTATTTTCCTTTTCCTAA

Downstream 100 bases:

>100_bases
TAATATAAACTAGGAATTAAATAGGGTGCTAATTTGCCAAAGAAAAGAGAAAATAGAGGAAGGAGAAAGGGAGACAAAGG
TCACGTAGGTTATATTTATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKVSKEKWEKNFSEWLDWVLREAEIYDYGRYPVKGMGVWMPYGFKIRQNVLQLIRKLLDETGHEEVLFPLLIPEDLLKKE
SEHIRGFEEEVYWVTKGGSQDLDVKLALRPTSETSITFMESFWVKSYKQLPKKYYQIVSVFRYETKATRPMMRLREITTF
KEAHTLHETYEDAARQVDEAINIYKAFFDELGIPYMISKRPEWDKFAGAEYTIAFDTILPDSRVLQIGTVHHLGQHFTKA
FDFKIQRKDGSLDYPHQTSYGISDRVIAVLIAINGDDHGPVLNPVIAPIKVVIVPIPAKDEETTAKIINYAKEVGENLKN
RGITVVIDDDKEKTPGEKFYIWELKGVPLRIEIGPKELNNNTVYIKRRDTFEGKSVPKDKAVEEVNTLLEKIKNDLHEKA
LKFLKERIIYTEDLNEAKKILEERAGVVEVPWCGDNNCGLQLQDVTNARVLGIPLDEDKDVSNAKCVMCKKPAKSLLRLA
KTY

Sequences:

>Translated_483_residues
MKVSKEKWEKNFSEWLDWVLREAEIYDYGRYPVKGMGVWMPYGFKIRQNVLQLIRKLLDETGHEEVLFPLLIPEDLLKKE
SEHIRGFEEEVYWVTKGGSQDLDVKLALRPTSETSITFMESFWVKSYKQLPKKYYQIVSVFRYETKATRPMMRLREITTF
KEAHTLHETYEDAARQVDEAINIYKAFFDELGIPYMISKRPEWDKFAGAEYTIAFDTILPDSRVLQIGTVHHLGQHFTKA
FDFKIQRKDGSLDYPHQTSYGISDRVIAVLIAINGDDHGPVLNPVIAPIKVVIVPIPAKDEETTAKIINYAKEVGENLKN
RGITVVIDDDKEKTPGEKFYIWELKGVPLRIEIGPKELNNNTVYIKRRDTFEGKSVPKDKAVEEVNTLLEKIKNDLHEKA
LKFLKERIIYTEDLNEAKKILEERAGVVEVPWCGDNNCGLQLQDVTNARVLGIPLDEDKDVSNAKCVMCKKPAKSLLRLA
KTY
>Mature_483_residues
MKVSKEKWEKNFSEWLDWVLREAEIYDYGRYPVKGMGVWMPYGFKIRQNVLQLIRKLLDETGHEEVLFPLLIPEDLLKKE
SEHIRGFEEEVYWVTKGGSQDLDVKLALRPTSETSITFMESFWVKSYKQLPKKYYQIVSVFRYETKATRPMMRLREITTF
KEAHTLHETYEDAARQVDEAINIYKAFFDELGIPYMISKRPEWDKFAGAEYTIAFDTILPDSRVLQIGTVHHLGQHFTKA
FDFKIQRKDGSLDYPHQTSYGISDRVIAVLIAINGDDHGPVLNPVIAPIKVVIVPIPAKDEETTAKIINYAKEVGENLKN
RGITVVIDDDKEKTPGEKFYIWELKGVPLRIEIGPKELNNNTVYIKRRDTFEGKSVPKDKAVEEVNTLLEKIKNDLHEKA
LKFLKERIIYTEDLNEAKKILEERAGVVEVPWCGDNNCGLQLQDVTNARVLGIPLDEDKDVSNAKCVMCKKPAKSLLRLA
KTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=498, Percent_Identity=31.7269076305221, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1786392, Length=162, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71991342, Length=489, Percent_Identity=31.4928425357873, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71991346, Length=355, Percent_Identity=34.0845070422535, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321808, Length=501, Percent_Identity=31.936127744511, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24649466, Length=502, Percent_Identity=31.2749003984064, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24649468, Length=484, Percent_Identity=31.8181818181818, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SULTO (Q971B5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377399.1
- HSSP:   O26708
- ProteinModelPortal:   Q971B5
- SMR:   Q971B5
- GeneID:   1459472
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1440
- NMPDR:   fig|273063.1.peg.1567
- HOGENOM:   HBG334108
- OMA:   GIPYPEE
- ProtClustDB:   PRK08661
- BioCyc:   STOK273063:ST1440-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 55822; Mature: 55822

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSKEKWEKNFSEWLDWVLREAEIYDYGRYPVKGMGVWMPYGFKIRQNVLQLIRKLLDE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
TGHEEVLFPLLIPEDLLKKESEHIRGFEEEVYWVTKGGSQDLDVKLALRPTSETSITFME
CCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHH
SFWVKSYKQLPKKYYQIVSVFRYETKATRPMMRLREITTFKEAHTLHETYEDAARQVDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INIYKAFFDELGIPYMISKRPEWDKFAGAEYTIAFDTILPDSRVLQIGTVHHLGQHFTKA
HHHHHHHHHHCCCCEEECCCCCCCHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHH
FDFKIQRKDGSLDYPHQTSYGISDRVIAVLIAINGDDHGPVLNPVIAPIKVVIVPIPAKD
HCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCEEEEEEECCCCC
EETTAKIINYAKEVGENLKNRGITVVIDDDKEKTPGEKFYIWELKGVPLRIEIGPKELNN
CHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCEEEEEECHHHCCC
NTVYIKRRDTFEGKSVPKDKAVEEVNTLLEKIKNDLHEKALKFLKERIIYTEDLNEAKKI
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHH
LEERAGVVEVPWCGDNNCGLQLQDVTNARVLGIPLDEDKDVSNAKCVMCKKPAKSLLRLA
HHHCCCEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHH
KTY
HCC
>Mature Secondary Structure
MKVSKEKWEKNFSEWLDWVLREAEIYDYGRYPVKGMGVWMPYGFKIRQNVLQLIRKLLDE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
TGHEEVLFPLLIPEDLLKKESEHIRGFEEEVYWVTKGGSQDLDVKLALRPTSETSITFME
CCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHH
SFWVKSYKQLPKKYYQIVSVFRYETKATRPMMRLREITTFKEAHTLHETYEDAARQVDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INIYKAFFDELGIPYMISKRPEWDKFAGAEYTIAFDTILPDSRVLQIGTVHHLGQHFTKA
HHHHHHHHHHCCCCEEECCCCCCCHHCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHH
FDFKIQRKDGSLDYPHQTSYGISDRVIAVLIAINGDDHGPVLNPVIAPIKVVIVPIPAKD
HCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCEEEEEEECCCCC
EETTAKIINYAKEVGENLKNRGITVVIDDDKEKTPGEKFYIWELKGVPLRIEIGPKELNN
CHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCEEEEEECHHHCCC
NTVYIKRRDTFEGKSVPKDKAVEEVNTLLEKIKNDLHEKALKFLKERIIYTEDLNEAKKI
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHH
LEERAGVVEVPWCGDNNCGLQLQDVTNARVLGIPLDEDKDVSNAKCVMCKKPAKSLLRLA
HHHCCCEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHH
KTY
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479